Effect of heat treatment of young plants on freeing chrysanthemums from virus B by means of meristem culture

Hakkaart, F. A.; Quak, F.

doi:10.1007/bf01979140

Cited by 28 publications

(6 citation statements)

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“…Meristem tip culture and thermotherapy did not give satisfactory number of virus -free plants. These results are in contrast with the earlier findings (Paludan 1973, Hakkaart and Quak 1964, Goethals et al 1973, Asatani 1972) that these methods could produce 90 -100 % CVB -free plants.…”

Section: ⎯⎯⎯⎯contrasting

confidence: 86%

“…Dahlia, freesia, geranium, lily, etc., have been raised virus-free using shoot tip meristems (Paludan 1973, Walkey 1991. Thermotherapy along with meristem tip culture has been used to raise virus-free carnation, narcissus, chrysanthemum and others (Hakkaart and Quak 1964). Thermotherapy at 36 -40 °C along with meristem tip culture has been also used to produce Chrysanthemum B carlavirus (CVB)-free plants (Asatani 1972, Goethals et al 1973.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

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Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm -3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.Additional key words: antiviral compounds, Chrysanthemum B carlavirus, Chrysanthemum morifolium, DAS-ELISA, in vitro production, RT-PCR.

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Section: ⎯⎯⎯⎯contrasting

confidence: 86%

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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“…Tissue culture methods are well established for virus elimination [3] and micropropagation [4,5] of chrysanthemum. In addition, there are numerous reports of adventitious shoot regeneration from various explants of chrysanthemum but in most cases shoots were produced from a callus intermediary [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.)

Kaul¹,

Miller²,

Hutchinson³

et al. 1990

Plant Cell Tiss Organ Cult

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Adventitious shoots were regenerated from leaf and stem explants of eleven chrysanthemum cultivars. The optimum medium for both explant types contained Murashige & Skoog basal medium supplemented with 5 ~M 6-benzylaminopurine and 5/~M e-napthaleneacetic acid. Generally, stem explants were superior to leaf explants. There were large cultivar differences in shoot regeneration frequency with three cultivars failing to respond over a wide range of hormone combinations. Shoots on stem explants appeared mainly to originate from cortical cells which rapidly divided and ruptured the epidermis. Regenerated shoots could be easily rooted, transferred to glasshouse conditions, and grown to flowering. All regenerated plants had the same morphological characteristics compared to plants derived from nodes.

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“…It was first of interest as a means of eliminating virus diseases from chrysanthemum stock plants (Hakkaart and Quak, 1964). It was first of interest as a means of eliminating virus diseases from chrysanthemum stock plants (Hakkaart and Quak, 1964).…”

mentioning

confidence: 99%

“…In vitro culture of the chrysanthemum has been possible for several years. It was first of interest as a means of eliminating virus diseases from chrysanthemum stock plants (Hakkaart and Quak, 1964). Later workers (Ben-Jaacov and Langhans, 1972) investigated its potential as a method of propagation.…”

mentioning

confidence: 99%

Plantlets from Petal Segments, Petal Epidermis, and Shoot Tips of the Periclinal Chimera, Chrysanthemum morifolium 'Indianapolis'

Bush

Earle

Langhans

1976

American Journal of Botany

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Cultivated chrysanthemums, especially the greenhouse series of ‘Indianapolis’ cultivars, are probably periclinal chimeras for flower color. Therefore, in vitro propagation of chrysanthemum, which has recently been described, might produce plants not true to type. To test this, plantlets were generated from cultures of petal segments, petal epidermis, and shoot tips; these plantlets were grown to flowering to determine whether chrysanthemums with two genetically different chimeral layers in the petals are stable in tissue culture. Layer I displaced layer II in the formation of new meristematic areas in shoot tip and petal culture, showing that such chimeras are unstable in culture. Many more abnormal morphological types were exhibited by the plants which were regenerated from petal cultures rather than those from shoot tip cultures. Abnormalities included quilled and incised petal forms, as well as lack of anthocyanin pigmentation, characteristics which may not be attributable to the rearrangement of chimeral layers. Paramutation, true mutation, and environmenal effects are offered as possible explanations for this phenomenon.

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Effect of heat treatment of young plants on freeing chrysanthemums from virus B by means of meristem culture

Cited by 28 publications

References 0 publications

Indexing and production of virus-free chrysanthemums

Indexing and production of virus-free chrysanthemums

Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.)

Plantlets from Petal Segments, Petal Epidermis, and Shoot Tips of the Periclinal Chimera, Chrysanthemum morifolium 'Indianapolis'

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