− AISI 4140 steel is widely used in various mechanical components owing to its superior mechanical properties. Surface hardening techniques are often used to further improve the properties, particularly for applications with moving components. The aim of this research is to understand the effect of heat treatment process on surface properties and tribological characteristics of AISI 4140 steel. In this work, we prepare two different AISI 4140 steel specimens-one cooled by air and one by oil-and determine surface properties such as surface topography and roughness using a confocal microscope. We also observe the cross-sections of the specimens using a scanning electron microscope to understand the difference in the material structure. In addition, we assess the hardness with respect to the distance from the surface using a micro-Vickers hardness tester. After characterizing the surfaces of the specimens, we investigate the wear characteristics of the specimens under hydrodynamic lubrication. The results show the presence of grooves on the surface of the oil-cooled specimens. It is likely that such grooves are formed during the cooling process using the oil. However, we observe no other significant differences in the surface properties of the specimens. The wear test results show the occurrence of severe wear on the oil-cooled specimens, which may be due to the groove formed on the surface. The results of this work may be helpful to improve surface properties using surface hardening techniques from a tribological perspective.