Effect of Heat Treatment on the Anticancer Activity of <i>Houttuynia cordata</i> Thunb Aerial Stem Extract in Human Gastric Cancer SGC-7901 Cells

Liu, Jinjuan; Zhu, Xiao‐Feng; Yang, Daning; Li, Rongpeng; Jiang, Jihong

doi:10.1080/01635581.2020.1737153

Cited by 21 publications

(15 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diamidino-2-phenylindole (DAPI) staining was carried out as previously described ( 16 ), and Mitochondrial Membrane Potential (MMP) was also measured by Rh-123 ( 17 ). Cells were first added to 6-well plates for adherence and incubated at 37°C for 24 h in an incubator containing 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Effects of Syringic Acid on Apoptosis, Inflammation, and AKT/mTOR Signaling Pathway in Gastric Cancer Cells

et al. 2021

View full text Add to dashboard Cite

Gastric cancer is one of the most common cancer and deadly disease worldwide. Despite substantial advances made in the treatment of gastric cancer, existing therapies still encounter bottlenecks. Chemotherapy, for instance, could lead to serious side effects, high drug resistance and treatment failure. Phytochemical-derived compounds from plants offer novel strategies as potent drug molecules in cancer therapy. Given the low toxicity and higher tolerance rate of naturally occurring compounds, the present study evaluated the effects of syringic acid on cytotoxicity, oxidative stress, mitochondrial membrane potential, apoptosis, and inflammatory responses in gastric cancer cell line (AGS). AGS cells were treated with various concentrations (5–40 μg/mL) of syringic acid for 24 h, after which cytotoxicity was analyzed. Reactive Oxygen Species (ROS), antioxidant enzyme activities, mitochondrial membrane potential (MMP, Δψm), cell morphologies, the expression of apoptotic markers and protein expression patterns were also investigated. Results indicated that syringic acid-treated cells developed anti-cancer activities by losing MMP, cell viability, and enhancing intracellular ROS. Syringic acid selectively developed apoptosis in a dose-dependent manner via enhanced regulation of caspase-3, caspase-9 and Poly ADP-ribose Polymerase (PARP) whereas decreasing the expression levels of p53 and BCL-2. Syringic acid also lowered activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) whereas Thio Barbituric Acid Reactive Substances (TBARS) increased. Syringic acid suppressed gastric cancer cell proliferation, inflammation, and induced apoptosis by upregulating mTOR via AKT signaling pathway. The study suggests syringic acid may constitute a promising chemotherapeutic candidate for gastric cancer treatment. Our study is the first report on the anti-cancer effects of syringic acid against gastric cancer cells via apoptosis, inhibition of inflammation, and the suppression of the mTOR/AKT signaling pathway.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of Syringic Acid on Apoptosis, Inflammation, and AKT/mTOR Signaling Pathway in Gastric Cancer Cells

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In early apoptosis, up-regulation of Bax can stimulate the formation of apoptosome complex via release of cytochrome c into cytosol, which then activates caspase-9 as initiator prior to cleaved activation of caspase-3 as effector [ 38 – 40 ]. Samana et al [ 10 ] and Jinjuan et al [ 12 ] reported that L. edodes and H. cordata extracts can significantly induce the apoptosis of SGC-7901 cells via up-regulation of Bax/Bcl-2 ratio at protein levels and activation of caspase-3. In the present study, we found the significant down-regulation of Bcl-2 expression and the significant up-regulation of Bax , Caspase-3 , and Caspase-9 and increase in Bax/Bcl-2 ratio following PBMF treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Aerial stem extracts of H. cordata Thunb. reportedly exert anticancer effects on GC by inducing apoptosis in SGC-7901 cells [ 12 ]. Dandelion root extracts can specifically suppress the proliferation and migration of SGC-7901 and BGC-823 GC cells [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

A plant-based medicinal food inhibits the growth of human gastric carcinoma by reversing epithelial–mesenchymal transition via the canonical Wnt/β-catenin signaling pathway

Chen

Yue

Tian

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Natural products, especially those with high contents of phytochemicals, are promising alternative medicines owing to their antitumor properties and few side effects. In this study, the effects of a plant-based medicinal food (PBMF) composed of six medicinal and edible plants, namely, Coix seed, Lentinula edodes, Asparagus officinalis L., Houttuynia cordata, Dandelion, and Grifola frondosa, on gastric cancer and the underlying molecular mechanisms were investigated in vivo. Methods A subcutaneous xenograft model of gastric cancer was successfully established in nude mice inoculated with SGC-7901 cells. The tumor-bearing mice were separately underwent with particular diets supplemented with three doses of PBMF (43.22, 86.44, and 172.88 g/kg diet) for 30 days. Tumor volumes were recorded. Histopathological changes in and apoptosis of the xenografts were evaluated by hematoxylin and eosin staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining, respectively. Serum levels of TNF-α, MMP-2, and MMP-9 were detected by enzyme-linked immunosorbent assay. The mRNA expression levels of β-catenin, GSK-3β, E-cadherin, N-cadherin, MMP-2/9, Snail, Bax, Bcl-2, Caspase-3/9, and Cyclin D1 were evaluated via real-time quantitative polymerase chain reaction. The protein expression levels of GSK-3β, E-cadherin, N-cadherin, and Ki-67 were determined by immunohistochemistry staining. Results PBMF treatment efficiently suppressed neoplastic growth, induced apoptosis, and aggravated necrosis in the xenografts of SGC-7901 cells. PBMF treatment significantly decreased the serum levels of MMP-2 and MMP-9 and significantly increased that of TNF-α. Furthermore, PBMF treatment notably upregulated the mRNA expression levels of GSK-3β, E-cadherin, Bax, Caspase-3, and Caspase-9 but substantially downregulated those of β-catenin, N-cadherin, MMP-2, MMP-9, Snail, and Cyclin D1 in tumor tissues. The Bax/Bcl-2 ratio was upregulated at the mRNA level. Moreover, PBMF treatment remarkably increased the protein expression levels of GSK-3β and E-cadherin but notably reduced those of Ki-67 and N-cadherin in tumor tissues. Conclusions The PBMF concocted herein exerts anti-gastric cancer activities via epithelial–mesenchymal transition reversal, apoptosis induction, and proliferation inhibition. The underlying molecular mechanisms likely rely on suppressing the Wnt/β-catenin signaling pathway.

show abstract

“…Interestingly, researchers compared the anti-gastric carcinoma activity of heated and unheated parts of H. cordata separately through DAPI staining and the detection of apoptosis and apoptotic protein levels, and cell viability decreased in SGC-7901, HepG2, NCI-H640, and HO-8910 cells with the increase of the extract concentration at 0, 25, 50, 100, and 150 ml/L. Furthermore, heated ariel stems showed 3–15 times higher effects than stems that were not heated in SGC-7901 cells, and the morphological characteristics of apoptosis, p53 protein, pro-apoptotic protein Bax, Bid, Bak, Apaf-1, activation of PARP, caspase9, and caspase3 were also increased, and the heated sample seemed to have higher activity than the unheated sample ( Liu et al, 2020 ). The above findings suggested the potential of H. cordata in preventing gastric carcinoma in the diet.…”

Section: Pharmacological Activitymentioning

confidence: 99%

Houttuynia cordata Thunb: An Ethnopharmacological Review

Zhao

Deng

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Houttuynia cordata Thunb (H. cordata; Saururaceae) is widely distributed in Asian regions. It plays an important role in traditional health care and disease treatment, as its aboveground stems and leaves have a long medicinal history in China and are used in the treatment of pneumonia and lung abscess. In clinical treatment, it can usually be combined with other drugs to treat dysentery, cold, fever, and mumps; additionally, H. cordata is an edible plant. This review summarizes detailed information on the phytochemistry and pharmacological effects of H. cordata. By searching the keywords “H. cordata and lung”, “H. cordata and heart”, “H. cordata and liver”, and “H. cordata and inflammation” in PubMed, Web of Science and ScienceDirect, we screened out articles with high correlation in the past ten years, sorted out the research contents, disease models and research methods of the articles, and provided a new perspective on the therapeutic effects of H. cordata. A variety of its chemical constituents are characteristic of medicinal plants, the chemical constituents were isolated from H. cordata, including volatile oils, alkaloids, flavonoids, and phenolic acids. Flavonoids and volatile oils are the main active components. In pharmacological studies, H. cordata showed organ protective activity, such as reducing the release of inflammatory factors to alleviate lung injury. Moreover, H. cordata regulates immunity, enhances the immune barriers of the vagina, oral cavity, and intestinal tract, and combined with the antibacterial and antiviral activity of its extract, effectively reduces pathogen infection. Furthermore, experiments in vivo and in vitro showed significant anti-inflammatory activity, and its chemical derivatives exert potential therapeutic activity against rheumatoid arthritis. Antitumour action is also an important pharmacological activity of H. cordata, and studies have shown that H. cordata has a notable effect on lung tumour, liver tumour, colon tumour, and breast tumour. This review categorizes the biological activities of H. cordata according to modern research papers, and provides insights into disease prevention and treatment of H. cordata.

show abstract

Effect of Heat Treatment on the Anticancer Activity of Houttuynia cordata Thunb Aerial Stem Extract in Human Gastric Cancer SGC-7901 Cells

Cited by 21 publications

References 36 publications

Effects of Syringic Acid on Apoptosis, Inflammation, and AKT/mTOR Signaling Pathway in Gastric Cancer Cells

Effects of Syringic Acid on Apoptosis, Inflammation, and AKT/mTOR Signaling Pathway in Gastric Cancer Cells

A plant-based medicinal food inhibits the growth of human gastric carcinoma by reversing epithelial–mesenchymal transition via the canonical Wnt/β-catenin signaling pathway

Houttuynia cordata Thunb: An Ethnopharmacological Review

Contact Info

Product

Resources

About