Effect of Heavy Metal Uptake by E. coli and Bacillus sps

Vijayadeep, C; Ps, Sastry

doi:10.4172/2155-6199.1000238

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…However, E. coli showed decreased cellular viability from the fourth amendment probably in response to platinum toxicity. This is not unusual as a previous study had shown a reduction in E. coli growth when treated with several toxic metals including Cu, Cd, Zn, and Hg (1-5 ppm) [26]. In this study, E. coli was found to be capable platinum biomineralization.…”

Section: Discussionsupporting

confidence: 76%

“…Most E. coli strains have been shown to be sensitive to heavy metals such as Zn, Cd, and Hg; studies have also shown that exposure of E. coli to Pt complexes (e.g. (NH 4 ) 2 [PtCl 6 ]) results in the inhibition of cell division [25][26][27][28][29]. However, some E. coli strains have been shown to exhibit a range of tolerances to different concentrations of Zn(II), Cd(II), Co(II), Ni(II) [27].…”

Section: Introductionmentioning

confidence: 99%

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Biomineralization of Platinum by Escherichia coli

Shar

Reith

Shahsavari

et al. 2019

Metals

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The widespread use of platinum in many industrial applications has led to its release into the environment at elevated concentrations with potential adverse effects on human and environmental health. However, the nature of interactions between mobile platinum complexes and the biotic components of the environment, which are increasingly being exposed to platinum, is poorly studied. The aim of this study was to assess the impact of Pt(IV)-chloride on the growth and activity of the well-characterized bacteria Escherichia coli. Bacterial survival and viability in the presence of different concentrations of Pt(IV)-chloride were assessed in liquid culture, while platinum retention was assessed using experimentation with sand-filled columns with the residual platinum concentration measured by atomic absorption spectroscopy. Bacterial biomineralization of platinum was studied with scanning electron microscopy. The results showed that E. coli tolerated PtCl4 at concentrations of up to 10,000 µM over 21 days and remained viable after 112 days of incubation with PtCl4 at 10,000 µM in sand columns. Overall, 74 wt.% and 50 wt.% of platinum was mineralized in E. coli and blank sand columns, respectively. The results of this study confirm that E. coli is capable of biomineralizing platinum. The results confirm that the interaction of platinum with bacteria is not limited to known metal-resistant bacterial species.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Biomineralization of Platinum by Escherichia coli

Shar

Reith

Shahsavari

et al. 2019

Metals

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“…Instead, they cause damage on different levels of the organism. By translocation of the mercury ions through the cell membranes, the intracellular metal-binding sites are exposed (Gourdon et al 1990) that ultimately can result in death of sensitive organisms unless a means of detoxification is induced or already possessed (Vijayadeep and Sastry 2014). The damage is mostly due to the avidity of the mercuric ions for the sulfhydryl groups of proteins, which they block and inactivate and to high affinity for phosphate groups and active groups of ADP or ATP, and for replacement of Fe from some iron-sulfur clusters (Patra and Sharma 2000;Nabi 2014).…”

Section: Introductionmentioning

confidence: 99%

Stoichiometry and kinetics of mercury uptake by photosynthetic bacteria

2017

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Mercury adsorption on the cell surface and intracellular uptake by bacteria represent the key first step in the production and accumulation of highly toxic mercury in living organisms. In this work, the biophysical characteristics of the mercury bioaccumulation are studied in intact cells of photosynthetic bacteria by use of analytical (dithizone) assay and physiological photosynthetic markers (pigment content, fluorescence induction and membrane potential) to determine the amount of mercury ions bound to the cell surface and taken up by the cell. It is shown that the Hg(II) uptake mechanism 1) has two kinetically distinguishable components, 2) includes co-opted influx through heavy metal transporters since the slow component is inhibited by Ca 2+ channel blockers, 3) shows complex pH-dependence demonstrating the competition of ligand binding of Hg(II) ions with H + ions (low pH) and high tendency of complex formation of Hg(II) with hydroxyl ions (high pH) and 4) is not a passive but an energy-dependent process as evidenced by light-activation and inhibition by protonophore. Photosynthetic bacteria can accumulate Hg(II) in amounts much (about 10 5 ) greater than their own masses by well defined strong and weak binding sites with equilibrium binding constants in the range of 1 (μM) -1 and 1 (mM) -1 , respectively. The strong binding sites are attributed to sulfhydryl groups as the uptake is blocked by use of sulfhydryl modifying agents and their number is much (two orders of magnitude) smaller than the number of weak binding sites. Biofilms developed by some bacteria (e.g. Rvx. gelatinosus) increase the mercury binding capacity further by a factor of about five. Photosynthetic bacteria in the light act as sponge of Hg(II) and can be potentially used for biomonitoring and bioremediation of mercury contaminated aqueous cultures.2

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“…Many metals are essential for growth of microorganisms in lower concentrations, yet are toxic in higher concentrations. Many microorganisms have the ability to selectively accumulate metals by the process of biosorption which involves the building or adsorption of heavy metals to living or dead cells (Vijayadeep and Sastry, 2014). The concentrations of the heavy metals analysed in the petroleum sludge in this study may not have affected the microbial growth in the overall biodegradation process.…”

Section: Resultsmentioning

confidence: 89%

Bioremediation of Petroleum Oil Sludge Polluted Brackish Water Ecosystem

Wokem¹,

Odokuma²,

Ariole³

2019

Int.J.Curr.Microbiol.App.Sci

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Petroleum oil sludge resulting from crude oil storage, illegal crude oil refining and bunkering activities constitutes environmental hazards and pollution in the crude oil producing communities in the Niger Delta region of Nigeria. Biostimulation with N.P.K. fertilizer option C, bioargumentation with indigenous hydrocarbon utilizing bacteria (HUB) option B, combination of biostimulation and bioaugmentation as option A and option D was without any bioremediation treatment were employed in the bioremediation of brackish water artificially polluted with petroleum oil sludge. Brackish water sample was obtained from Elechi Creek, Port Harcourt Rivers State. Petroleum oil sludge sample was obtained from Crude Oil Processing Plant in Obegi community, Rivers State. Bioremediation was monitored for 56 days using the percentage ratio of total petroleum hydrocarbon (TPH) losses for each period to TPH loss at day 0. The result of physicochemical analysis of the petroleum sludge showed that aliphatic hydrocarbon (n-alkanes) ranged from C 13 -C 35, with concentrations ranging from 26.12-7,713.62ppmwith TPH of 89,509.9ppm. The polycyclic aromatic hydrocarbon (PAH) range was 0.03-5.36ppm with total concentration of 24.21ppm. Heavy metal analysis showed; iron (49.42mg/kg), Zinc (3.79mg/kg), Nickel (4.53 mg/kg) and manganese (6.90 mg/kg). The average total heterotrophic bacterial (THB) and (HUB) counts for petroleum sludge were; 2.5 x 10 5 cfu/g and 2.0 x10 5 cfu/g and for the brackish water sample were 1.39 x 106cfu/ml and 1.1 x 10 4 cfu/ml respectively. Statistical analysis (ANOVA) showed that the THB and HUB counts were significantly different at 5 percent levels (P<0.05) in the different treatment options during the bioremediation period. Changes in physico-chemical parameters showed that pH, alkalinity, conductivity, chemical oxygen demand, nitrate and phosphate were significantly different (P<0.05) while there were no significant differences (P>0.05) in the following parameter; salinity biochemical oxygen demand and total hydrocarbon continent.Using least significant difference (LSD), treatment option D and the control option E were different from treatments A, B and C. The phylogenetic analysis identification of the HUB isolates implicated in the degradation process revealed a closely related ness to the following organisms, Lysinibacillus sphaericus, Klebsiella pneumonia and Alcaligenes faecalis of different strains. The bacterial sequences submitted to Genbank were assigned Accession Number KX817218-KXV7225. The percentage losses in TPH from Gas Chromatography (GC) results showed the following; option A (91.8%), option B (92.5%), C (95%) D (57.8%) and option E control (39.5%) respectively. The results suggest that the application of biostimulation with N.P.K fertilizer, bioaugmentation with indigenous HUB or a combination of both will enhance the bioremediation of petroleum sludge polluted brackish water system in the Niger Delta of Nigeria.

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Effect of Heavy Metal Uptake by E. coli and Bacillus sps

Cited by 13 publications

References 6 publications

Biomineralization of Platinum by Escherichia coli

Biomineralization of Platinum by Escherichia coli

Stoichiometry and kinetics of mercury uptake by photosynthetic bacteria

Bioremediation of Petroleum Oil Sludge Polluted Brackish Water Ecosystem

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