Probiotics are alive bacteria that have a healthy effect on the hosts and are found in large quantities in the soil and foods. The most recommended probiotics as commercial are the some species of Lactobacillus sp., Bifidobacterium sp., and Streptococcus sp. The fatty acids are formed by the colonic gut flora from dietary fibres, which manage to escape the host's enzymatic digestive systems in the small intestine. Dietary fibres that manage to reach the large intestine are available for several bacterial fermentative reactions. The fermentation of the different dietary fibres leads to an increase in concentrations of several short-chain fatty acids, especially butyrate, propionate and acetate, in the lumen of the proximal regions of the large intestine. In this review, we acquainted that the relationship between fatty acids and probiotic bacteria affects some various health ailments.