Effect of heavy metals on protein content of marine unicellular green alga Dunaliella tertiolecta

Agawany, Nagwa El; Kaamoush, Mona; El-Zeiny, Ahmed M.; Ahmed, Mahmoud S.

doi:10.1007/s10661-021-09353-y

Cited by 22 publications

(8 citation statements)

References 26 publications

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“…The algal pellet was re-suspended in 5 mL distilled water and then boiled for 2 h. After refrigeration, the water extract was centrifuged at 15,000× g at 4 • C for 15 min. The clear supernatant was used for the spectrophotometric determination of soluble protein concentration as previously described [26].…”

Section: Protein Extraction and Determinationmentioning

confidence: 99%

Sulfur Starvation in Extremophilic Microalga Galdieria sulphuraria: Can Glutathione Contribute to Stress Tolerance?

Salbitani

Perrone

Rosati

et al. 2022

Plants

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This study reports the effects of sulfur (S) deprivation in cultures of Galdieria sulphuraria (Cyanidiophyceae). Galdieria is a unicellular red alga that usually grows, forming biomats on rocks, in S-rich environments. These are volcanic areas, where S is widespread since H2S is the prevalent form of gas. The glutathione content in Galdieria sulphuraria is much higher than that found in the green algae and even under conditions of S deprivation for 7 days, it remains high. On the other hand, the S deprivation causes a decrease in the total protein content and a significant increase in soluble protein fraction. This suggests that in the conditions of S starvation, the synthesis of enzymatic proteins, that metabolically support the cell in the condition of nutritional stress, could be up regulated. Among these enzymatic proteins, those involved in cell detoxification, due to the accumulation of ROS species, have been counted.

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Section: Protein Extraction and Determinationmentioning

confidence: 99%

Sulfur Starvation in Extremophilic Microalga Galdieria sulphuraria: Can Glutathione Contribute to Stress Tolerance?

Salbitani

Perrone

Rosati

et al. 2022

Plants

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“…Urea is a relatively common nitrogen source in the ocean. After being absorbed by algae, it can usually accumulate in the algae, and rely on the action of urease to decompose urea into CO 2 , which is then absorbed as a reduced nitrogen source [17].…”

Section: The Effect Of Nitrogen and Phosphorusmentioning

confidence: 99%

Effects of Nitrogen and Phosphorus Nutrients on the Proliferation of Heterosigma Akashiwo under the Background of Mobile Big Data

Duan

Huang

Zheng

et al. 2022

International Transactions on Electrical Energy Systems

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In the era of information explosion, mobile data is developing rapidly, and many fields are facing the challenges of data collection, analysis and operation. Heterosigma akashiwo is a kind of wide temperature and wide salt algae, belonging to the class Echinococcus and the genus heterobenthos. It is widely distributed in the coastal waters of the world. It has formed harmful red tides for many times in many countries, causing a large number of fish deaths, causing great economic losses and serious damage to aquatic resources. If toxic red tides break out frequently, they will pose a great threat to public health. Seawater eutrophication is the main environmental inducement for the formation of red tide by Heterosigma akashiwo. In particular, the content of nutrients in seawater significantly affects the reproduction of Heterosigma akashiwo and the excessive proliferation of plankton such as algae. At the same time, a large number of planktons will devour the dissolved oxygen in the water, thus affecting the photosynthesis of the water body, resulting in the deterioration of the water quality and the damage to the functions of ecology and water resources. As for the effects of nitrogen, phosphorus, iron and different vitamins on the growth of Heterosigma akashiwo, this paper takes Heterosigma akashiwo as the research object, and focuses on the analysis of the effects of ammonium chloride, urea and sodium dihydrogen phosphate on the proliferation of Heterosigma akashiwo. e results showed that the higher or lower the concentration of nitrogen and phosphorus, the slower the proliferation rate of Heterosigma akashiwo; Heterosigma akashiwo had the maximum proliferation rate under the nitrogen concentration of 300-500 μmol/L; At the concentration of 10∼15 μmol/L phosphorus, its growth rate is the fastest and its proliferation level is the highest. e selected nitrogen and phosphorus have a very significant impact on the proliferation of microalgae.

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“…Determination of the half-maximal effective concentration of heavy metal (EC50) is very important for the estimation of metal toxicity which can use EC50 values of some heavy metals such as Cu, Ni, and Zn determined for growth inhibition of some green algae species to determine their toxicity, for example Cu found to be more toxic to algae than Ni or Zn (Magdaleno et al 2014 ; El Agawany et al 2021 ). Mechanisms underlying metal tolerance in algae are not fully recognized and may rely on several mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Role of zinc as an essential microelement for algal growth and concerns about its potential environmental risks

El-Agawany

Kaamoush

2022

Environ Sci Pollut Res

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This work aims to measure the role of zinc as an essential micronutrient for algal growth and the effect of using different concentrations of this heavy metal on growth and essential metabolites of Dunaliella tertiolecta. The EC50 obtained was around 15 mg/l. The results obtained proved that lower concentrations of the element increased growth and the content of the measured metabolites (photosynthesis pigments, fatty acids, and protein) but with different responses. The increase in content of these metabolic products with low concentrations of the tested heavy metal may be attributed to inhibition to these metabolites’ export out of cells by heavy metals. The obtained infrared peaks of the major cell constituents of the treated cells revealed the emergence of new peaks and the removal of others, indicating changes in cell constituents due to changing zinc concentrations.

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Effect of heavy metals on protein content of marine unicellular green alga Dunaliella tertiolecta

Cited by 22 publications

References 26 publications

Sulfur Starvation in Extremophilic Microalga Galdieria sulphuraria: Can Glutathione Contribute to Stress Tolerance?

Sulfur Starvation in Extremophilic Microalga Galdieria sulphuraria: Can Glutathione Contribute to Stress Tolerance?

Effects of Nitrogen and Phosphorus Nutrients on the Proliferation of Heterosigma Akashiwo under the Background of Mobile Big Data

Role of zinc as an essential microelement for algal growth and concerns about its potential environmental risks

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