Effect of Helicobacter pylori-Associated Chronic Gastritis on Autonomous Activity and Sleep Quality in Mice

Abstract

Many reports have shown that patients with Hp-associated chronic gastritis exhibit anxiety and poor sleep quality. However, less is known about the effects and specific manifestations of Hp-associated chronic gastritis on autonomous activity and sleep quality in animals. Here, we investigated the effect of Helicobacter pylori (Hp)-associated chronic gastritis on autonomous activity and sleep quality in mice. To do this, a Hp-associated chronic gastritis mouse model was first established, then analyzed for autonomous activity, relative to controls, for 15 min using an autonomous activity tester. Next, sleep quality of mice was detected by sodium pentobarbital-induced sleep experiment and results compared between groups. The results showed that male mice in the model group exhibited higher activity counts but lower forelimb lift counts, relative to those in the control group, although there were no significant differences (all p > .05). Conversely, female mice in the model group recorded lower activity counts, albeit at no significant difference (p > .05), and significantly lower counts of forelimb lift (p < .05), relative to those in the control group. Notably, male mice in the model group had longer sleep latency and shorter sleep duration than those in the control group, albeit at no significant differences (all p > .05). On the other hand, female mice in the model group recorded significantly longer sleep latency as well as shorter sleep duration compared to those in the control group (all p < .01). We conclude that Hp-associated chronic gastritis exerts certain effects on autonomous activity and sleep quality of mice in a gender-dependent manner. Notably, female mice with Hp-associated chronic gastritis had lower activity and forelimb lift counts, as well as prolonged sleep latency, and shortened sleep duration. These effects were all statistically significant except for activity counts.

Research on the signaling pathway and the related mechanism of traditional Chinese medicine intervention in chronic gastritis of the “inflammation-cancer transformation”

“…The combination inhibits abnormal activation of the Wnt/β-catenin signalling pathway, thereby preventing excessive cell proliferation and differentiation, epithelial-mesenchymal transformation and reducing inflammation in gastric tissue. As a result, it provides protection to the gastric tissue ( Liu et al, 2022b ; Dilinaer et al, 2023 ).…”

Increased risk of sleep-related movement disorder in patients with Helicobacter pylori infection: A nationwide population-based study

“…Therefore, it has been proposed that gut microflora, including Helicobacter pylori may produce metabolites, trigger neuroinflammation, and affect the dopaminergic system by crossing the gut-brain axis ( 13 , 14 ). Notably, in a mouse model of HPI, women with HPI-associated chronic gastritis had lower activity and forelimb lift counts, as well as prolonged sleep latency and shortened sleep duration ( 15 ).…”