Effect of Helicobacter pylori infection on gastric mucosal phospholipid contents and their fatty acid composition

Abstract

To investigate the effect of Helicobacter pylori infection on the 'gastric mucosal barrier', phospholipid contents and the fatty acid composition of endoscopic biopsy specimens of the gastric mucosa were analysed in healthy volunteers with and without H. pylori infection. The gastric corporeal phosphatidylcholine (PC) content of H. pylori-positive healthy volunteers was less than that of H. pylori-negative healthy volunteers (P < 0.05). Moreover, H. pylori-positive healthy volunteers had a decrease in linoleic acid composition (P < 0.0001) and an increase in arachidonic acid composition (P < 0.0001) and in the arachidonic acid/linoleic acid ratio (P < 0.0001) of antral and corporeal PC compared with H. pylori-negative healthy volunteers. These findings suggest that H. pylori infection enhances production of various eicosanoids, resulting in changes in the gastric mucosal phospholipid contents and their fatty acid composition, that may consequently cause the gastric mucosal barrier to be weakened.

Helicobacter pylori alters n‐6 fatty acid metabolism and prostaglandin E2 synthesis in rat gastric mucosal cells

“…Nardone et al have reported that H. pylori infection does not significantly affect fatty acid composition of phospholipids in the gastric biopsy specimens from patients with non‐alcoholic liver cirrhosis 30 . However, in our previous study, 11 H. pylori infection markedly decreased linoleic acid composition and increased arachidonic acid composition of gastric mucosal PC. The reasons for the discrepancies are currently unclear.…”

“…The samples stored were then scraped in 1.5 mL PBS, and then homogenized. Phospholipid determination and fatty acid analysis were performed as previously described 11 . Briefly, total lipids of the whole cell were extracted according to the method of Bligh and Dyer 21 .…”

Inhibitory Activities and Attenuated Expressions of 5-LOX with Red Ginseng in Helicobacter pylori-Infected Gastric Epithelial Cells

“…It has been shown that colonization of H pylori in the gastric mucosa induces a dramatic change in gastric mucosal phospholipid composition and elevates the metabolites of various eicosanoids from epithelial membrane phospholipid, which has been related to inflammatory host responses in infected gastric environment [5,6]. These causal relationships were further proved by the fact that the eradication of H pylori reduces eicosanoid synthesis, resulting in the normalization of gastric mucosal phospholipid contents and its fatty acid composition [7,8].…”

Helicobacter pylori-induced Prostaglandin E2 Synthesis Involves Activation of Cytosolic Phospholipase A2 in Epithelial Cells

“…tients may play a crucial role in H. pylori infection (4). In gastrointestinal epithelia, PGE 2 is implicated in maintaining the normal function and structure of the gastric mucosa by modulating diverse cellular functions such as secretion of fluid and electrolytes, mucosal blood flow, and cell proliferation (5,6).…”