Effect of Helicobacter pylori infection on antral gastrin and somatostatin cells and on serum gastrin concentrations

Abstract

ObjectivesHelicobacter pylori infection induces selective reduction of the number of antral D-cells and results in abnormal regulation of serum gastrin secretion. The purpose of this study was to investigate the relationship between H. pylori infection and the numbers of G-cells and D-cells.MethodsThe numbers of antral G-cells and D-cells, the ratio of G-cells to D-cells and fasting serum gastrin concentrations were compared between 37 patients with (29 with duodenal ulcers and 8 with gastric ulcers) and 33 without H. pylori infection (22 with duodenal ulcers and 11 with gastric ulcers). Serum gastrin concentrations were measured using the radioimmunoassay technique. Antral mucosal biopsy specimens were examined using immunohistochemical staining with antibodies specific for gastrin and somatostatin and the numbers of G-cells and D-cells per gastric gland were counted.ResultsFasting serum gastrin concentrations were significantly higher in patients with H. pylori infection compared to patients without infection (80.3±23.5 vs 47.6±14.1 pg/ml, p<0.001). The number of G-cells per gastric gland was similar in infected and uninfected patients (7.1±3.1 vs 7.3±3.9, respectively, p>0.5). The number of D-cells was significantly lower in patients with H. pylori infection than in uninfected patients in both duodenal and gastric ulcer patients (1.3±0.4 vs 2.5±1.6, respectively, p<0.001). The ratio of G-cells to D-cells was also significantly higher in infected patients compared with uninfected patients for both gastric and duodenal ulcers (5.7±2.7 vs 3.5±1.9, respectively, p<0.001).ConclusionsThese results strongly suggest that Helicobacter pylori infection induces reduction of the number of antral D-cells. The resulting relative hypofunction of the inhibitory action of D-cells against G-cells may be responsible for increased serum gastrin secretion.

Synergistic effects of combined curcumin and antibiotic in ameliorating an animal model of Helicobacter pylori infection

“…A significant benefit of combined therapy on IL-4 more profound than single therapy alone suggested that combined therapy could reduce the effects of H. pylori infection more than each therapy alone. Gastrin is a hormone produced by G cells from the antrum and involved in the production of gastric acid [33]. Similar to current result, it is clear the levels of gastrin and gastric acid were increased by H. pylori infection.…”

A model for integrative study of human gastric acid secretion

“…The G cell-to-D cell ratio obtained from our immunohistochemistry analysis is similar to G cell-to-D cell ratios reported previously (26). Experimental evidence suggests that there are Ͻ10 of each endocrine cell type per gastric gland (26,77); therefore, the three-dimensional analysis used to estimate corpus D and parietal cells is not needed to estimate the numbers of antral cells. To deduce the number of G and D cells in the antrum, we assume that the antrum comprises 25% of the stomach, and, given the total number of gastric glands in the stomach (31), we calculate the number of each of the antral cell types (Table 1).…”

The Difference of Serum Gastrin-17 Level Based on Gastritis Severity and Helicobacter Pylori Infection

“…In Sheykholeslami et al, study, Gastrin-17 was increased in corpus-predominant gastritis (p < 0.01) [5]. However in this study, Gastrin-17 level was significantly higher in severe neutrophil infiltration.…”