Effect of heterogeneous multi-layered gelatin scaffolds on the diffusion characteristics and cellular activities of preosteoblasts

Ahn, Geunseon; Kim, Yunna; Lee, Sang‐Won; Jeong, Yoon Jeong; Son, Hyungbin; Lee, Dong-Hyun

doi:10.1007/s13233-014-2024-y

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…The hydrogel water up-take capability is consistent with the values reported for other, diversely crosslinked gelatin matrices for bone/tissue engineering (500–2000% swelling degree) [ 5 , 6 , 9 , 10 ]. The time needed for the materials to reach the maximum hydration is also in agreement with literature data [ 45 , 56 ].…”

Section: Discussionsupporting

confidence: 90%

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Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

Gatta

Tirino

Cammarota

et al. 2021

Regenerative Biomaterials

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Gelatin hydrogels by microbial-transglutaminase crosslinking are being increasingly exploited for tissue engineering, and proved high potential in bone regeneration. This study aimed to evaluate, for the first time, the combination of enzymatically crosslinked gelatin with hyaluronan and the newly developed biotechnological chondroitin in enhancing osteogenic potential. Gelatin enzymatic crosslinking was carried out in the presence of hyaluronan or of a hyaluronan–chondroitin mixture, obtaining semi-interpenetrating gels. The latter proved lower swelling extent and improved stiffness compared to the gelatin matrix alone, whilst maintaining high stability. The heteropolysaccharides were retained for 30 days in the hydrogels, thus influencing cell response over this period. To evaluate the effect of hydrogel composition on bone regeneration, materials were seeded with human dental pulp stem cells and osteogenic differentiation was assessed. The expression of osteocalcin (OC) and osteopontin (OPN), both at gene and protein level, was evaluated at 7, 15 and 30 days of culture. Scanning electron microscopy (SEM) and two-photon microscope observations were performed to assess bone-like extracellular matrix (ECM) deposition and to observe the cell penetration depth. In the presence of the heteropolysaccharides, OC and OPN expression was upregulated and a higher degree of calcified matrix formation was observed. Combination with hyaluronan and chondroitin improved both the biophysical properties and the biological response of enzymatically crosslinked gelatin, fastening bone deposition.

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Section: Discussionsupporting

confidence: 90%

“…Hydrogel stability to collagenase action was also evaluated as previously described [ 45 , 46 ]. Briefly, comparable amounts of the materials were incubated in a collagenase solution (3 U/mL) in PBS, at 37°C, and the weight loss (%) was monitored over incubation time.…”

Section: Methodsmentioning

confidence: 99%

Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

Gatta

Tirino

Cammarota

et al. 2021

Regenerative Biomaterials

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“…[ 379 ] The superior water solubility to collagen make gelatin convenient and robust for chemical functionalization and crosslinking. Inherited from collagen, gelatin has multiple cell‐interaction motifs [ 380 ] and can be digested by collagenase [ 381 ] in vivo, making it of interest for cell encapsulation. Moreover, gelatin is reported to be nonantigenic and nonimmunogenic, due to the absence of aromatic rings after hydrolyzation.…”

Section: Polymer Precursorsmentioning

confidence: 99%

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

2021

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Hydrogels are an important class of biomaterials with the unique property of high‐water content in a crosslinked polymer network. In particular, chemically crosslinked hydrogels have made a great clinical impact in past years because of their desirable mechanical properties and tunability of structural and chemical properties. Various polymers and step‐growth crosslinking chemistries are harnessed for fabricating such covalently crosslinked hydrogels for translational research. However, selecting appropriate crosslinking chemistries and polymers for the intended clinical application is time‐consuming and challenging. It requires the integration of polymer chemistry knowledge with thoughtful crosslinking reaction design. This task becomes even more challenging when other factors such as the biological mechanisms of the pathology, practical administration routes, and regulatory requirements add additional constraints. In this review, key features of crosslinking chemistries and polymers commonly used for preparing translatable hydrogels are outlined and their performance in biological systems is summarized. The examples of effective polymer/crosslinking chemistry combinations that have yielded clinically approved hydrogel products are specifically highlighted. These hydrogel design parameters in the context of the regulatory process and clinical translation barriers, providing a guideline for the rational selection of polymer/crosslinking chemistry combinations to construct hydrogels with high translational potential are further considered.

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“…The main goal of tissue engineering is to design, construct, regenerate and repair damaged tissues in the human body (Abdullah et al 2009;Ahn et al 2014). …”

Section: Introductionmentioning

confidence: 99%

“…They play a crucial role in the reconstruction of diseased tissues due to their porous, biocompatible and non-toxic characteristics. They also must have an interconnected porous network for easy access of nutrients into the cells and removal of metabolic wastes such as lactate from the cells (Ahn et al 2014). As the amount of interconnected pores varies in different tissue engineering scaffolds, the nutrient diffusivity in these scaffolds may also vary (Suhaimi et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Glucose diffusivity in cell-seeded tissue engineering scaffolds

Suhaimi

Das

2015

Biotechnol Lett

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6Objective A series of well-defined diffusion experiments was carried out to determine the effective 7 glucose diffusion coefficient in cell-seeded porous scaffolds to understand the importance of nutrient 8 diffusion in tissue engineering bioreactor. 9Results Cell growth changed the morphological structure of the scaffolds reducing the effective pore 10 space and inevitably decreased the effective glucose diffusivity in the chosen scaffolds, namely, collagen, 11 poly(L-lactide) and poly(caprolactone) scaffolds from 3.71 x 10 -9 m 2

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Effect of heterogeneous multi-layered gelatin scaffolds on the diffusion characteristics and cellular activities of preosteoblasts

Cited by 11 publications

References 26 publications

Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

Glucose diffusivity in cell-seeded tissue engineering scaffolds

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