Effect of Hexadecane-induced Vesiculation on the Outer Membrane of Acinetobacter calcoaceticus

Borneleit, Petra; Hermsdorf, Thomas; Claus, R.; Walther, Paul; Kleber, Hans‐Peter

doi:10.1099/00221287-134-7-1983

Cited by 13 publications

(5 citation statements)

References 24 publications

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“…For N. meningitidis it was shown that cysteine depletion causes oxidative stress which triggered OMV release [81]. Also a number of media components can induce OMV production such as hexadecane for Acinetobacter [83], glucose for Yersinia [32, 84] and sodium carbonate for V. cholerae [85]. In P. aeruginosa the signal molecule PQS promotes OMV release [59], or under anoxic conditions, when no PQS can be synthesized, the bactericidal toxin pyocin [86].…”

Section: Natural Omvmentioning

confidence: 99%

Outer membrane vesicles as platform vaccine technology

Pol

Stork

Ley

2015

Biotechnology Journal

288

241

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Outer membrane vesicles (OMVs) are released spontaneously during growth by many Gram‐negative bacteria. They present a range of surface antigens in a native conformation and have natural properties like immunogenicity, self‐adjuvation and uptake by immune cells which make them attractive for application as vaccines against pathogenic bacteria. In particular with Neisseria meningitidis, they have been investigated extensively and an OMV‐containing meningococcal vaccine has recently been approved by regulatory agencies. Genetic engineering of the OMV‐producing bacteria can be used to improve and expand their usefulness as vaccines. Recent work on meningitis B vaccines shows that OMVs can be modified, such as for lipopolysaccharide reactogenicity, to yield an OMV product that is safe and effective. The overexpression of crucial antigens or simultaneous expression of multiple antigenic variants as well as the expression of heterologous antigens enable expansion of their range of applications. In addition, modifications may increase the yield of OMV production and can be combined with specific production processes to obtain high amounts of well‐defined, stable and uniform OMV particle vaccine products. Further improvement can facilitate the development of OMVs as platform vaccine product for multiple applications.

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Section: Natural Omvmentioning

confidence: 99%

Outer membrane vesicles as platform vaccine technology

Pol

Stork

Ley

2015

Biotechnology Journal

288

241

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“…The release of DNA-containing OMVs from pathogenic species of Acinetobacter has also been previously reported (25)(26)(27). Acinetobacter baylyi (previously also denoted Acinetobacter calcoaceticus) has been described to release OMVs when grown on hexadecane (28).…”

mentioning

confidence: 98%

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

Fulsundar

Harms

Flaten

et al. 2014

Appl Environ Microbiol

211

197

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dOuter membrane vesicles (OMVs) are continually released from a range of bacterial species. Numerous functions of OMVs, including the facilitation of horizontal gene transfer (HGT) processes, have been proposed. In this study, we investigated whether OMVs contribute to the transfer of plasmids between bacterial cells and species using Gram-negative Acinetobacter baylyi as a model system. OMVs were extracted from bacterial cultures and tested for the ability to vector gene transfer into populations of Escherichia coli and A. baylyi, including naturally transformation-deficient mutants of A. baylyi. Anti-double-stranded DNA (anti-dsDNA) antibodies were used to determine the movement of DNA into OMVs. We also determined how stress affected the level of vesiculation and the amount of DNA in vesicles. OMVs were further characterized by measuring particle size distribution (PSD) and zeta potential. Transmission electron microscopy (TEM) and immunogold labeling were performed using antifluorescein isothiocyanate (anti-FITC)-conjugated antibodies and anti-dsDNA antibodies to track the movement of FITC-labeled and DNA-containing OMVs. Exposure to OMVs isolated from plasmid-containing donor cells resulted in HGT to A. baylyi and E. coli at transfer frequencies ranging from 10 ؊6 to 10 ؊8 , with transfer efficiencies of approximately 10 3 and 10 2 per g of vesicular DNA, respectively. Antibiotic stress was shown to affect the DNA content of OMVs as well as their hydrodynamic diameter and zeta potential. Morphological observations suggest that OMVs from A. baylyi interact with recipient cells in different ways, depending on the recipient species. Interestingly, the PSD measurements suggest that distinct size ranges of OMVs are released from A. baylyi.

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“…5). Examples of this are the chaperones that assist in proper protein folding and functioning, and the intracellular trafficking, secretion and vesicular transport transcripts that are involved in production of outer membrane vesicles formation through blebbing (Toyofuku et al ., 2019) which can eject toxic agents when bacteria are grown on ADOC‐rich or other hydrophobic compound‐rich media (Borneleit et al ., 1988; Kobayashi et al ., 2000).…”

Section: Resultsmentioning

confidence: 99%

Bacterial responses to background organic pollutants in the northeast subarctic Pacific Ocean

Martinez-Varela

Cerro‐Gálvez

Auladell

et al. 2021

Environmental Microbiology

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Thousands of man-made synthetic chemicals are released to oceans and compose the anthropogenic dissolved organic carbon (ADOC). Little is known about the effects of this chronic pollution on marine microbiome activities. In this study, we measured the pollution level at three sites in the Northeast Subarctic Pacific Ocean (NESAP) and investigated how mixtures of three model families of ADOC at different environmentally relevant concentrations affected naturally occurring marine bacterioplankton communities' structure and metabolic functioning. The offshore northernmost site (North) had the lowest concentrations of hydrocarbons, as well as organophosphate ester plasticizers, contrasting with the two other continental shelf sites, the southern coastal site (South) being the most contaminated. At North, ADOC stimulated bacterial growth and promoted an increase in the contribution of some Gammaproteobacteria groups (e.g. Alteromonadales) to the 16 rRNA pool. These groups are described as fast responders after oil spills. In contrast, minor changes in South microbiome activities were observed. Gene expression profiles at Central showed the coexistence of ADOC degradation and stress-response strategies to cope with ADOC toxicities. These results show that marine microbial communities at three distinct domains in NESAP are influenced by background concentrations of ADOC, expanding previous assessments for polar and temperate waters.

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Effect of Hexadecane-induced Vesiculation on the Outer Membrane of Acinetobacter calcoaceticus

Cited by 13 publications

References 24 publications

Outer membrane vesicles as platform vaccine technology

Outer membrane vesicles as platform vaccine technology

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

Bacterial responses to background organic pollutants in the northeast subarctic Pacific Ocean

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