Effect of Hexose Analogs on Growth of Chilomonas paramecium

Abstract

SYNOPSIS. Observations on the effect of 7 glucose analogs on growth of C. paramecium showed that 2‐deoxy‐D‐glucose and 2‐deoxy‐D‐galactose inhibited growth. Inhibition in 2‐DG was not influenced by pH, calcium concentration, or by carbon sources as lactate, acetate, n‐butyl alcohol, or succinate. Similarly, glucose or glucose‐6‐phosphate did not reverse inhibition. Organisms were viable in 2‐DG only when a carbon source in the basal medium was absent. Wherever a useful carbon source was present, 2‐DG was lethal in concentrations over 40 mg/100 ml. Phosphorylation of 2‐DG did not occur. Lactic acid dehydrogenase was not inhibited. Glucose‐6‐phosphate dehydrogenase appears to have an affinity for 2‐DG; phosphohexose isomerase activity was decreased by 2‐DG.