Effect of Hg2+ on HydSL Hydrogenase of the Purple Sulfur Bacteria Thiocapsa roseopersicina BBS

Zorin, N. A.; Starodubov, A. S.; Цыганков, А. А.

doi:10.1134/s0003683820020155

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Figures 2C, 4). Although it does not reach the stability of Thiocapsa roseopersicina (half-life time of 60 days at 24 • C) (Zorin and Gogotov, 1982) or Alteromonas macleodii (100% enzyme activity after 45 days at 4 • C) (Vargas et al, 2011), SP11F2 exhibits a higher oxygen-tolerance and stability than the [NiFe]-hydrogenase of S. oneidensis (and the oxygen-sensitive majority of hydrogen converting enzymes; Vignais and Billoud, 2007), if immobilized on PAN fiber electrodes.…”

Section: Applying Hydrogen Converting Enzymes From An Uncultured Ventmentioning

confidence: 99%

Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage

Adam

Schlicht

Han

et al. 2020

Front. Bioeng. Biotechnol.

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Hydrogen can in the future serve as an advantageous carrier of renewable energy if its production via water electrolysis and utilization in fuel cells are realized with high energy efficiency and non-precious electrocatalysts. In an unprecedented novel combination of structured electrodes with hydrogen converting enzymes from the uncultured and thus largely inaccessible microbial majority (>99%) we address this challenge. The geometrically defined electrodes with large specific surface area allow for low overpotentials and high energy efficiencies to be achieved. Enzymatic hydrogen evolution electrocatalysts are used as alternatives to noble metals. The enzymes are harnessed from the environmental microbial DNA (metagenomes) of hydrothermal vents exhibiting dynamic hydrogen and oxygen concentrations and are recovered via a recently developed novel activity-based screening tool. The screen enables us to target currently unrecognized hydrogenase enzymes from metagenomes via direct expression in a surrogate host microorganism. This circumvents the need for cultivation of the source organisms, the primary bottleneck when harnessing enzymes from microbes. One hydrogen converting metagenome-derived enzyme exhibited high activity and unusually high stability when dispersed on a TiO 2-coated polyacrylonitrile fiber electrode. Our results highlight the tremendous potential of enzymes derived from uncultured microorganisms for applications in energy conversion and storage technologies.

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Section: Applying Hydrogen Converting Enzymes From An Uncultured Ventmentioning

confidence: 99%

Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage

Adam

Schlicht

Han

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

Reconstruction of HydSL Hydrogenase from Thiocapsa roseopersicina after Cyanide Inhibition

Зорин

Khusnutdinova

Starodubov

et al. 2021

Appl Biochem Microbiol

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Comparison of HydSl Hydrogenase of Thiocapsa bogorovii and its Modification with a Truncated C-Terminus of HydS

Starodubov

Khasimov

Khusnutdinova

et al. 2022

Appl Biochem Microbiol

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Effect of Hg2+ on HydSL Hydrogenase of the Purple Sulfur Bacteria Thiocapsa roseopersicina BBS

Cited by 3 publications

References 13 publications

Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage

Metagenomics Meets Electrochemistry: Utilizing the Huge Catalytic Potential From the Uncultured Microbial Majority for Energy-Storage

Reconstruction of HydSL Hydrogenase from Thiocapsa roseopersicina after Cyanide Inhibition

Comparison of HydSl Hydrogenase of Thiocapsa bogorovii and its Modification with a Truncated C-Terminus of HydS

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