Effect of high-concentrate diet on amino acid transporter expression and milk quality in Holstein dairy cows

Xie, Zheng; Ye, P S; Zhang, Y S; Shen, Xiang

doi:10.4238/2015.may.18.16

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Figure 2A shows that the average SCC was higher in HC-fed cattle, achieving 166,733 cells/mL, but did not achieve the threshold of 205,000 cells/mL (Rodrigues et al, 2009). These results were similar to those of our previous research, which showed that feeding a HC diet to cattle depressed milk quality (Xie et al, 2015b). However, in the present study, the SCC content and NAGase activity were higher than observed in our previous study (Xie et al, 2015b), because here we extended the experimental period.…”

Section: Discussionsupporting

confidence: 82%

“…These results were similar to those of our previous research, which showed that feeding a HC diet to cattle depressed milk quality (Xie et al, 2015b). However, in the present study, the SCC content and NAGase activity were higher than observed in our previous study (Xie et al, 2015b), because here we extended the experimental period. We therefore hypothesized that cattle mammary epithelial cells may suffer from sub-clinical mastitis when animals are fed a HC diet over a relatively long experimental period.…”

Section: Discussioncontrasting

confidence: 37%

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Effect of a high-concentrate diet on milk components and mammary health in Holstein dairy cows

Xie

Zhang

et al. 2017

Genet. Mol. Res.

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ABSTRACT. In order to evaluate the milk yield, milk quality, and health of dairy cows fed a high-concentrate (HC) diet, eight lactating Holstein dairy cattle were randomly assigned to HC or low-concentrate (LC) diet groups and fed for 50 days, and the autocontrol studying before and after treatment with the two diets was used. During the experiment, plasma and milk samples were collected and measured. With regard to milk component, HC feeding led to higher milk production (P < 0.05), but significantly lower milk protein percentage (P < 0.05), milk protein yield (P < 0.05), and milk fat percentage (P < 0.05) throughout the five periods than LC feeding. Milk somatic cell count and N-acetyl-D-glucosaminidase activity (P < 0.01) were higher than those observed under LC feeding. mRNA expression levels of interleukin-8 (IL-8), C-C motif chemokine ligand (CCL5), and lactalbumin alpha (a-LA) were investigated by qPCR and found to be significantly lower (P < 0.01) in cattle fed the HC diet. The amino acid content was analyzed by high performance liquid chromatography (HPLC), and the content of Asp (P < 0.01), Gln (P < 0.01), Ala (P < 0.05), Leu (P < 0.05), Lys (P < 0.05), and Ile (P < 0.01) was significantly lower in the HC group, whereas the content of Arg (P < 0.05) and Phe (P < 0.01) was significantly higher. These results suggest that the HC diet might have an important influence on mammary health. The amino acid content was lower, suggesting that depletion of amino acids, resulting in depleted milk protein, affects milk quality.

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Section: Discussionsupporting

confidence: 82%

Section: Discussioncontrasting

confidence: 37%

Effect of a high-concentrate diet on milk components and mammary health in Holstein dairy cows

Xie

Zhang

et al. 2017

Genet. Mol. Res.

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“…This result suggests that goats with SARA experience stress ( Dong et al., 2013 ; Jia et al., 2014 ). Furthermore, many studies have found that a high concentrate diet causes liver metabolic abnormalities, including glucose metabolism, lipid metabolism, and protein metabolism ( Dong et al., 2013 ; Xie et al., 2015 ). In addition, a high concentrate diet induces oxidative stress in the liver.…”

Section: Discussionmentioning

confidence: 99%

Whole transcriptome analysis of RNA expression profiles reveals the potential regulating action of long noncoding RNA in lactating cows fed a high concentrate diet

Yao

et al. 2021

Animal Nutrition

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Subacute ruminal acidosis (SARA) is a common metabolic disease in the dairy farming industry which is usually caused by an excessive amount of high concentrate diet. SARA not only threatens animal welfare but also leads to economic losses in the farming industry. The liver plays an important role in the distribution of nutritional substances and metabolism; however, a high concentrate diet can cause hepatic metabolic disorders and liver injury. Recently, noncoding RNA has been considered as a critical regulator of hepatic disease, however, its role in the bovine liver is limited. In this study, 12 mid-lactating dairy cows were randomly assigned to a control (CON) group (40% concentrate of dry matter, n = 6) and a SARA group (60% concentrate of dry matter, n = 6). After 21 d of treatment, all cows were sacrificed, and liver tissue samples were collected. Three dairy cows were randomly selected from the CON and SARA groups respectively to perform whole transcriptome analysis. More than 20,000 messenger RNA (mRNA), 10,000 long noncoding RNA (lncRNA), 3,500 circular RNA (circRNA) and 1,000 micro RNA (miRNA) were identified. Furthermore, 43 mRNA, 121 lncRNA and 3 miRNA were differentially expressed, whereas no obvious differentially expressed circRNA were detected between the 2 groups. Gene Ontology (GO) annotation revealed that the differentially expressed genes were mainly enriched in oxidoreductase activity, stress, metabolism, the immune response, cell apoptosis, and cell proliferation. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the deferentially expressed genes were highly enriched in the phosphatidylinositol 3 kinase ( PI3K ) - serine/threonine kinase ( AKT ) signaling pathway ( P < 0.05). According to KEGG pathway analysis, the differentially expressed lncRNA (DElncRNA) target genes were mainly related to proteasomes, peroxisomes, and the hypoxia-inducible factor-1 signaling pathway ( P < 0.005). Further bioinformatics and integrative analyses revealed that the lncRNA were strongly correlated with mRNA; therefore, it is reasonable to speculate that lncRNA potentially play important roles in the liver dysfunction induced by SARA. Our study provides a valuable resource for future investigations on the mechanisms of SARA to facilitate an understanding of the importance of lncRNA, and offer functional RNA information.

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Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle

et al. 2021

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Effect of high-concentrate diet on amino acid transporter expression and milk quality in Holstein dairy cows

Cited by 5 publications

References 32 publications

Effect of a high-concentrate diet on milk components and mammary health in Holstein dairy cows

Effect of a high-concentrate diet on milk components and mammary health in Holstein dairy cows

Whole transcriptome analysis of RNA expression profiles reveals the potential regulating action of long noncoding RNA in lactating cows fed a high concentrate diet

Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle

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