Effect of high electron donor supply on dissimilatory nitrate reduction pathways in a bioreactor for nitrate removal

Behrendt, Anna; Tarre, Sheldon; Beliavski, Michael; Green, Michal; Klatt, Judith M.; Beer, Dirk de; Stief, Peter

doi:10.1016/j.biortech.2014.08.073

Cited by 32 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Behrendt et al (2014) performed denitrifying batch experiments, with a high acetate:nitrate ratio in the medium, in which no DNRA was observed. Akunna et al (1993) performed mixed culture batch experiments for varying C-sources at similar initial amounts of COD.…”

Section: Dissimilative Nitrate Reductionmentioning

confidence: 99%

Enrichment of DNRA bacteria in a continuous culture

et al. 2015

View full text Add to dashboard Cite

Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are competing microbial nitrate-reduction processes. The occurrence of DNRA has been shown to be effected qualitatively by various parameters in the environment. A more quantitative understanding can be obtained using enrichment cultures in a laboratory reactor, yet no successful DNRA enrichment culture has been described. We showed that a stable DNRA-dominated enrichment culture can be obtained in a chemostat system. The enrichment was based on the hypothesis that nitrate limitation is the dominant factor in selecting for DNRA. First, a conventional denitrifying culture was enriched from activated sludge, with acetate and nitrate as substrates. Next, the acetate concentration in the medium was increased to obtain nitrate-limiting conditions. As a result, conversions shifted from denitrification to DNRA. In this selection of a DNRA culture, two important factors were the nitrate limitation and a relatively low dilution rate (0.026 h − 1 ). The culture was a highly enriched population of Deltaproteobacteria most closely related to Geobacter lovleyi, based on 16S rRNA gene sequencing (97% similarity). We established a stable and reproducible cultivation method for the enrichment of DNRA bacteria in a continuously operated reactor system. This enrichment method allows to further investigate the DNRA process and address the factors for competition between DNRA and denitrification, or other N-conversion pathways.

show abstract

Section: Dissimilative Nitrate Reductionmentioning

confidence: 99%

Enrichment of DNRA bacteria in a continuous culture

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Another study also observed no positive removal of NH 4 -N, when the influent NH 4 -N level was below 0.41 mg/L [40]. The increase in NH 4 -N concentration might be due to dissimilatory nitrate reduction to ammonia, which is a reduction process that could reduce NO 2 -N and NO 3 -N back to NH 4 -N under anoxic/reduced environments [41]. In this study, the increase in COD concentrations and high COD/NO 3 -N ratio could favor the production of NH 4 -N through dissimilatory nitrate reduction.…”

Section: Nitrogen Removalmentioning

confidence: 96%

Peat as Substrate for Small-Scale Constructed Wetlands Polishing Secondary Effluents from Municipal Wastewater Treatment Plant

Jin

Carlos

McConnell

et al. 2017

Water

View full text Add to dashboard Cite

Abstract:With the recent development of constructed wetland technology, it has become a mainstream treatment technology for the mitigation of a variety of wastewaters. This study reports on the treatment performance and pH attenuation capacity of three different configurations of small-scale on-site surface flow constructed wetlands (SFCW): T1 (Peat + Typha latifolia), T2 (T. latifolia alone), and T3 (Peat alone) treating secondary effluent from the Amherstview Water Pollution Control Plant (WPCP) for two treatment periods (start-up period and operational period). The aim of this study was to compare the nutrients removal efficiencies between the different treatments, as well as to evaluate the effects of substrate and vegetation on the wetland system. For a hydraulic retention time of 2.5 days, the results showed that all treatment systems could attenuate the pH level during both the start-up and operational periods, while significant nutrient removal performance could only be observed during the operational period. Peat was noted to be a better SFCW substrate in promoting the removal of nitrate (NO 3 -N), total nitrogen (TN), and phosphorus. The addition of T. latifolia further enhanced NO 3 -N and TN removal efficiencies, but employing T. latifolia alone did not yield effluents that could meet the regulatory discharge limit (1.0 mg/L) for phosphorus.

show abstract

“…This result indicated that high nitrate loading might favor denitrifiers re-domination in the reactor. In the stage V and VI, relative higher influent nitrate loading and more electron donors used by denitrifiers would also enhance denitrification performance, as it is more energy-gained pathway in such circumstance (Behrendt et al, 2014). Another reason might be that the high influent flow rate reduced the thickness of biofilm and brought in certain DO which suppressed DNRA activity (Wu et al, 2013).…”

Section: Long-term Denitrification Performance Of the Two Reactorsmentioning

confidence: 98%

Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment

Zhu

Deng

Ruan

et al. 2015

Bioresource Technology

133

View full text Add to dashboard Cite

Effect of high electron donor supply on dissimilatory nitrate reduction pathways in a bioreactor for nitrate removal

Cited by 32 publications

References 29 publications

Enrichment of DNRA bacteria in a continuous culture

Enrichment of DNRA bacteria in a continuous culture

Peat as Substrate for Small-Scale Constructed Wetlands Polishing Secondary Effluents from Municipal Wastewater Treatment Plant

Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment

Contact Info

Product

Resources

About