Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams

Cheng, Adrienne A; Li, W.; Hernández, Laura

doi:10.1152/physiolgenomics.00052.2018

Cited by 13 publications

(11 citation statements)

References 66 publications

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“…A hyperenergetic diet can alter the gene expression profile in the MG during lactation and thus modify the synthesis of milk components and subsequent milk composition. In particular, the expression of a variety of collagen genes was disrupted in the MG of female mice fed a high-fat diet, which is consistent with previous studies in other tissues such as the liver and heart [ 27 , 37 , 38 ]. After a high-fat diet, mammary tissue exhibits an inflammatory process that can lead to impaired synthesis and secretion of milk.…”

Section: Effects Of Energetic Supplementationsupporting

confidence: 90%

“…Moreover, MGs from lactating rats fed a high-fat diets displayed a disrupted morphology characterized by a few and small alveoli and were associated with a decrease in the transcription process in basal epithelial cells, which are important to the differentiation of the MG [ 26 ]. Although the measurement of milk production is difficult in models such as rodents, a high-fat diet consumed from puberty to mid-lactation showed no significant differences in milk yield during the first half of lactation [ 27 ]. Nevertheless, effects of high-fat diet tightly depend on species.…”

Section: Effects Of Energetic Supplementationmentioning

confidence: 99%

“…Endocrine and molecular studies have shown that a high-fat diet increases PKC activity and estrogen receptor level in mammary tissue [ 35 , 36 ]. Furthermore, consumption of a high-fat diet alters the transcriptome of mammary tissue at the peak of lactation and creates a proinflammatory environment [ 27 ]. A hyperenergetic diet can alter the gene expression profile in the MG during lactation and thus modify the synthesis of milk components and subsequent milk composition.…”

Section: Effects Of Energetic Supplementationmentioning

confidence: 99%

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Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species

Hue-Beauvais

Faulconnier

Charlier

et al. 2021

Genes

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In mammals, milk is essential for the growth, development, and health. Milk quantity and quality are dependent on mammary development, strongly influenced by nutrition. This review provides an overview of the data on nutritional regulations of mammary development and gene expression involved in milk component synthesis. Mammary development is described related to rodents, rabbits, and pigs, common models in mammary biology. Molecular mechanisms of the nutritional regulation of milk synthesis are reported in ruminants regarding the importance of ruminant milk in human health. The effects of dietary quantitative and qualitative alterations are described considering the dietary composition and in regard to the periods of nutritional susceptibly. During lactation, the effects of lipid supplementation and feed restriction or deprivation are discussed regarding gene expression involved in milk biosynthesis, in ruminants. Moreover, nutrigenomic studies underline the role of the mammary structure and the potential influence of microRNAs. Knowledge from three lactating and three dairy livestock species contribute to understanding the variety of phenotypes reported in this review and highlight (1) the importance of critical physiological stages, such as puberty gestation and early lactation and (2) the relative importance of the various nutrients besides the total energetic value and their interaction.

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Section: Effects Of Energetic Supplementationsupporting

confidence: 90%

Section: Effects Of Energetic Supplementationmentioning

confidence: 99%

Section: Effects Of Energetic Supplementationmentioning

confidence: 99%

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Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species

Hue-Beauvais

Faulconnier

Charlier

et al. 2021

Genes

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“…The effect of collagen gene alterations on the lactating mammary gland and their subsequent effect on the infant is unknown. Two different studies have investigated collagen expression during peak lactation in mouse milk or the mammary gland, both demonstrating that an HFD alters collagen gene expression during peak lactation (5,6). Here we show that in general, Tph1-KO HFD mice have decreased collagen gene expression in comparison to WT-HFD mice.…”

Section: Discussionmentioning

confidence: 52%

Transcriptomic analysis investigating the interaction between peripheral serotonin and high-fat diet feeding on mammary gene expression in midlactation mice

Cheng

Hernández

2020

Physiological Genomics

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To understand the role of peripheral serotonin and its interaction with diet in midlactation mammary gene expression, our study uses tryptophan hydroxylase 1 knockout ( Tph1-KO) mice fed a high-fat diet (HFD). It has previously been demonstrated that HFD feeding increases inflammatory and immune pathways in peak lactation mammary glands of mice and increases pup mortality in wild-type (WT) mice compared with dams fed a low-fat diet (LFD). Peripheral serotonin inhibition has been associated with resistance to obesity in male mice fed an HFD. Little is known about the function of Tph1 and how peripheral serotonin affects mammary gland function during pregnancy and lactation. In this study, WT and Tph1-KO models were used to investigate global transcriptomic changes in peak lactation mammary glands when dams were fed either an HFD or LFD. WT and Tph1-KO female mice were assigned to either an LFD or HFD beginning at 3 wk of age ( n = 4/group). Dams were euthanized on lactation day 11. Differentially expressed genes (DEGs) were first filtered by adjusted P value (cutoff ≤ 0.05) and fold-change (FC, cutoff ≥2). Genes were further filtered by mean normalized read count with a cutoff 310. We did not observe many differentially expressed genes in WT and Tph1-KO dams fed LFD. However, 3,529 DEGs were observed between WT-HFD and Tph1-KO-HFD mice, including cell cycle regulation and MAPK pathways being significantly enriched. Further research is required to completely understand the physiological significance of our results on peak lactation mammary physiology and the contribution of serotonin.

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“…A number of studies have reported that thousands of genes are differentially expressed by a high-fat diet as compared to a control diet (50)(51)(52)(53). We profiled global gene expression by RNA-Seq in three female mice fed the AIN-93M diet and three female mice fed a HFCA diet from each CC founder strains (n = 48 in total).…”

Section: Hepatic Transcriptomics Reveals Diet-specific Differences In the CC Progenitorsmentioning

confidence: 99%

Hepatic transcriptional profile reveals the role of diet and genetic backgrounds on metabolic traits in female progenitor strains of the Collaborative Cross

Kim

Huda

O’Connor

et al. 2021

Physiological Genomics

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Mice have provided critical mechanistic understandings of clinical traits underlying Metabolic Syndrome (MetSyn) and susceptibility to MetSyn in mice is known to vary among inbred strains. We investigated the diet- and strain-dependent effects on metabolic traits in the eight Collaborative Cross (CC) founder strains (A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HILtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ). Liver transcriptomics analysis showed that both atherogenic diet and host genetics have profound effects on the liver transcriptome, which may be related to differences in metabolic traits observed between strains. We found strain differences in circulating trimethylamine N-Oxide (TMAO) concentration and liver triglyceride content, both of which are traits associated with metabolic diseases. Using a network approach, we identified a module of transcripts associated with TMAO and liver triglyceride content which was enriched in functional pathways. Interrogation of the module related to metabolic traits identified NADPH oxidase 4 (Nox4), a gene for a key enzyme in the production of reactive oxygen species, which showed a strong association with plasma TMAO and liver triglyceride. Interestingly, Nox4 was identified as the highest expressed in the C57BL/6J and NZO/HILtJ strains and the lowest expressed in the CAST/EiJ strain. Based on these results, we suggest that there may be genetic variation in the contribution of Nox4 to the regulation of plasma TMAO and liver triglyceride content. In summary, we show that liver transcriptomic analysis identified diet- or strain-specific pathways for metabolic traits in the Collaborative Cross (CC) founder strains.

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Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams

Cited by 13 publications

References 66 publications

Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species

Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species

Transcriptomic analysis investigating the interaction between peripheral serotonin and high-fat diet feeding on mammary gene expression in midlactation mice

Hepatic transcriptional profile reveals the role of diet and genetic backgrounds on metabolic traits in female progenitor strains of the Collaborative Cross

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