Effect of high-glucose concentrations on the expression of collagens and fibronectin by fibroblasts in culture

Benazzoug, Yasmina; Borchiellini, Carole; Labat‐Robert, J.; Robert, L.; Kern, Patrick

doi:10.1016/s0531-5565(98)00015-1

Cited by 42 publications

(31 citation statements)

References 38 publications

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“…Our findings corroborate those of Chen et al (2000) [2], who noted an increase in matrix proteins, Benazzoug et al (1998) [35] noted an increase in total collagen in human cutaneous fibroblasts incubated in 15 Mm Glc for 48 h. Aouichat et al (2004) [7] also noted an increase in total collagen in SMCs of Psammomys incubated in 15 mM Glc for 48 h and 4 passages. The Typing of collagen showed an increase and intensification of bands corresponding to the γ fragments representing the trimer chain α1 (coll I and III), the β fragments representing the dimeric chain α1 (coll I and III), α1 chains (coll I and III) and α2 chain (coll I).…”

Section: Open Accesssupporting

confidence: 91%

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Impact of glucotoxicity induced in vivo and in vitro in Psammomys obesus

Berdja¹,

Smail²,

Kheira³

et al. 2012

JDM

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Objective: Chronic hyperglycemia characteristic of type diabetes 2 is responsible for the accelerated atherosclerosis with increased cardiovascular risk. In this study, we will propose to analyze the effect of a long-term of glucotoxicity in vivo in Psammomys obesus by addition of sucrose to 30% for 11 months and in vitro study of adventitial fibroblasts in the presence of D-glucose 0.6% for 7 days. Materials and methods: Evaluation of plasma biochemical parameters was carried out at the initial time and at the end of experiment. At autopsy, a morphological study of the aorta was performed after fixation in aqueous Bouin and staining with Masson's trichrome. The experimental glucotoxicity is induced by incubation of fibroblasts in DMEM enriched with D-glucose at 0.6% for 7 days. The impact of glucotoxicity is assessed in the intracellular compartments through dosage of total nitrite and malondialdehyde, a product of lipid peroxidation, and thanks to a morphological assay after fixation of cells with aqueous bouin and blood staining with May Grünwald Giemsa. The evaluation of cell proliferation is accomplished by cell counting. Collagens I and III of the extracellular compartment are characterized by SDS-PAGE. Results: Animals subjected to sucrose showed hyperglycemia associated with hyperinsulinemia, dyslipidemia, hyperproteinemia, increased CPK and VLDL-LDL and decreased HDL. Histology of aortas revealed endothelial cells hypertrophy, severe disorganization of intima and media. In the presence of glucose, the proliferation of fibroblasts increases very significantly (P = 2.34 × 10

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Section: Open Accesssupporting

confidence: 91%

“…This increase is indicative of increased synthesis of cellular protein. Benazzoug, (1998) [35] noted little change in total protein synthesis of incubated human fibroblasts 15Mm Glc. Hoffman et al (1998) [36] noted that hyperglycemia stimulates the synthesis of many growth factors including TGF-β in mice mesangial cells.…”

Section: Open Accessmentioning

confidence: 98%

Impact of glucotoxicity induced in vivo and in vitro in Psammomys obesus

Berdja¹,

Smail²,

Kheira³

et al. 2012

JDM

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“…The metabolic effect of high glucose concentration results partially from the age-dependent modifications of the insulin-triggered signaling pathways (insulin resistance). We showed previously that high glucose concentration also modifies the biosynthesis of extracellular matrix components such as fibronectin and type III collagen [13]. A 3 g/l glucose concentration in the fibroblast culture medium produced a selective increase in type III collagen and the fibronectin biosynthesis, comparable to modifications seen for these 2 matrix components with in vitro aging of fibroblasts, that is increasing passage numbers (the Hayflick model [14]).…”

Section: Introductionmentioning

confidence: 79%

Effect of High Glucose Concentration on Corneal Collagen Biosynthesis

Robert

Ménasche²,

Robert³

et al. 2006

Ophthalmologica

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The effect of high glucose concentration (3 g/l) on bovine corneal total protein and collagen biosynthesis was studied, using ³H-proline incorporation in explant cultures with protein and collagen determinations. The high glucose concentration increased the incorporation of ³H-proline in total corneal proteins as well as in collagens. The specific radioactivity of stromal collagens was strongly increased in these conditions. Mannitol was used to control the osmotic effect of the high glucose concentration, both at 1 and 3 g/l concentrations. Mannitol did not increase the incorporation of ³H-proline in total proteins or collagens, but on the contrary decreased it. The high glucose concentration decreased the excretion of neosynthesized proteins and collagens in the culture medium, but did not affect the total protein or collagen content of the corneas. The strong increase in the specific radioactivity of corneal collagens in the presence of 3 g/l glucose suggests an increased turnover of collagens in diabetic corneas. The increased biosynthesis of collagens together with their decreased elimination in the extracellular compartment can create the conditions for the formation and accumulation of advanced glycation endproducts by the Maillard reaction. This can induce and stimulate the liquefaction of the vitreous body leading to sight-threatening disorders such as diabetic retinopathy, retinal detachment, glaucoma, cataract formation and age-related macular degeneration.

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“…31,32 Although metabolic memory has primarily been demonstrated in vascular cells, 33 there is some evidence that fibroblasts retain functional changes, such as altered ECM production, after exposure to high glucose. 34,35 Therefore, metabolic memory may play a role in maintaining the phenotype of DFU-derived fibroblasts in our in vitro tissue models. Further studies using these models are necessary to determine whether and how metabolic memory mediates the long-term effects of the DFU microenvironment manifested in the 3D models.…”

Section: D In Vitro Chronic Wound Modelsmentioning

confidence: 99%

Three-Dimensional Human Tissue Models That Incorporate Diabetic Foot Ulcer-Derived Fibroblasts Mimic In Vivo Features of Chronic Wounds

Maione

Brudno

Stojadinović

et al. 2015

Tissue Engineering Part C: Methods

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Diabetic foot ulcers (DFU) are a major, debilitating complication of diabetes mellitus. Unfortunately, many DFUs are refractory to existing treatments and frequently lead to amputation. The development of more effective therapies has been hampered by the lack of predictive in vitro methods to investigate the mechanisms underlying impaired healing. To address this need for realistic wound-healing models, we established patientderived fibroblasts from DFUs and site-matched controls and used them to construct three-dimensional (3D) models of chronic wound healing. Incorporation of DFU-derived fibroblasts into these models accurately recapitulated the following key aspects of chronic ulcers: reduced stimulation of angiogenesis, increased keratinocyte proliferation, decreased re-epithelialization, and impaired extracellular matrix deposition. In addition to reflecting clinical attributes of DFUs, the wound-healing potential of DFU fibroblasts demonstrated in this suite of models correlated with in vivo wound closure in mice. Thus, the reported panel of 3D DFU models provides a more biologically relevant platform for elucidating the cell-cell and cell-matrix-related mechanisms responsible for chronic wound pathogenesis and may improve translation of in vitro findings into efficacious clinical applications.

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Effect of high-glucose concentrations on the expression of collagens and fibronectin by fibroblasts in culture

Cited by 42 publications

References 38 publications

Impact of glucotoxicity induced <i>in vivo</i> and <i>in vitro</i> in <i>Psammomys obesus</i>

Impact of glucotoxicity induced <i>in vivo</i> and <i>in vitro</i> in <i>Psammomys obesus</i>

Effect of High Glucose Concentration on Corneal Collagen Biosynthesis

Three-Dimensional Human Tissue Models That Incorporate Diabetic Foot Ulcer-Derived Fibroblasts Mimic In Vivo Features of Chronic Wounds

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Effect of high-glucose concentrations on the expression of collagens and fibronectin by fibroblasts in culture

Cited by 42 publications

References 38 publications

Impact of glucotoxicity induced &lt;i&gt;in vivo&lt;/i&gt; and &lt;i&gt;in vitro&lt;/i&gt; in &lt;i&gt;Psammomys obesus&lt;/i&gt;

Impact of glucotoxicity induced &lt;i&gt;in vivo&lt;/i&gt; and &lt;i&gt;in vitro&lt;/i&gt; in &lt;i&gt;Psammomys obesus&lt;/i&gt;

Effect of High Glucose Concentration on Corneal Collagen Biosynthesis

Three-Dimensional Human Tissue Models That Incorporate Diabetic Foot Ulcer-Derived Fibroblasts Mimic In Vivo Features of Chronic Wounds

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Product

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Impact of glucotoxicity induced <i>in vivo</i> and <i>in vitro</i> in <i>Psammomys obesus</i>

Impact of glucotoxicity induced <i>in vivo</i> and <i>in vitro</i> in <i>Psammomys obesus</i>