Effect of high glucose on secreted proteome in cultured retinal pigmented epithelium cells: Its possible relevance to clinical diabetic retinopathy

Higher prolactin and vasoinhibin serum levels associated with incidence and progression of retinopathy of prematurity

“…Moreover, expression of matrix metalloproteases (MMP) 2 and 9 is enhanced in eyes of mice with experimental ROP before the vasoproliferative phase (30,31), and several MMP, including MMP-2 and MMP-9, cleave PRL to vasoinhibins (32). Cathepsin D, which also generates vasoinhibins (21), is also present in various eye compartments (33) and is downregulated in the circulation of patients with diabetic retinopathy (34). The fact that vasoinhibin levels are not elevated during the vasoproliferative phase of ROP suggests that PRL-cleaving activity becomes downregulated in this second phase which, coupled to higher PRL levels, may favor the proangiogenic outcome of PRL action.…”

Cathepsin D: an Mφ‐derived factor mediating increased endothelial cell permeability with implications for alteration of the blood‐retinal barrier in diabetic retinopathy

“…The level of this protein was higher in patients with DME compared to nondiabetic patients and diabetics without retinal complications. A previous study reported decreased plasma CD levels in patients with DR (60). Again, this study does not specify whether these patients had DME or not.…”

An easy, rapid method to isolate RPE cell protein from the mouse eye

“…Since collagen VI is a structural protein in the choroid, we next wanted to show that a soluble protein in the choroid, such as albumin or immunoglobulins, do not leak into the RPE lysates using the lysis buffer protocol. Since these and other soluble proteins from the circulation are expressed by the RPE(Anderson and Anderson, 2002; Chen et al, 2012; Niu et al, 2013), we instead intravenously injected human plasma containing lipoprotein(a), composed of apolipoprotein(a) and apolipoproteinB, and after 5 minutes, assessed Lp(a) contamination in the RPE after lysis buffer dissection. We specifically selected Lp(a) because mice do not produce Lp(a).…”