Effect of high growth temperature on Serratia marcescens

Abstract

The purpose of this study was to investigate some parameters of S. marcescens IBBPo15 cells adaptation to toxic organic solvents. S. marcescens IBBPo15 cells incubated at 37°C exhibited a slower growth when they were exposed to 5% organic solvents (alkanes, aromatics), as compared with the control cells. S. marcescens IBBPo15 cells grown at 37°C produced protease and the enzyme production decreased in cells exposed to aromatic compounds, as compared with the control cells. Surfactant serrawettin and red pigment prodigiosin were produced by S. marcescens IBBPo15 cells grown at 37°C. Modifications in the pigment and extracellular proteins profile were observed in S. marcescens IBBPo15 cells grown at 37°C during exposure to organic solvents (alkanes, aromatics). Alkane hydroxylase (i.e., alkB1) and toluene dioxygenase (i.e., todM) catabolic genes, 4'-phosphopantetheinyl transferase (i.e., pswP) and serralysin metalloproteinase (i.e., ser) genes were detected by polymerase chain reaction in DNA extracted from S. marcescens IBBPo15 control cells grown at 37°C, as well as in cells exposed to organic solvents (excepting todM in n-decane exposed cells).

Bioconversion of Waste Foolscap and Newspaper to Fermentable Sugar

“…Thus, we can deduce that Serratia operates best at the temperature between 37° and 40°C, since higher yield was recorded at these two temperatures. Although bacterial enzymes such Serratia used for this work have an optimum range of temperature that aid its microbial activities (37-45°C), this is seen from the work of [35] on the effect of high growth temperature on Serratia marcescens; therefore, the trend being studied is not a situation of a temperature increase above the optimum, but rather that 37°C is a more suitable temperature for high saccharification than 40°C and 45°C. However, the comparative analysis of the temperature and the metabolites shows that the 37°C is a more optimum temperature in the production of reducing sugar from the waste paper substrates and also that Serratia have the highest effects in the bioconversion on the paper samples used.…”