Effect of high hydrostatic pressure on extraction of B-phycoerythrin from Porphyridium cruentum: Use of confocal microscopy and image processing

Tran, Thierry; Denimal, Emmanuel; Lafarge, Céline; Journaux, Ludovic; Lee, John A.; Winckler, Pascale; Perrier-Cornet, Jean-Marie; Pradelles, Rémi; Loupiac, Camille; Cayot, Nathalie

doi:10.1016/j.algal.2018.101394

Cited by 16 publications

(7 citation statements)

References 45 publications

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“…In this study, these 10 objects will be named vesicles for more convenience. They were already observed in previous work [35].…”

Section: Confocal Fluorescence Microscopy Image Descriptionsupporting

confidence: 82%

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Ex situ and in situ investigation of protein/exopolysaccharide complex in Porphyridium cruentum biomass resuspension

Tran¹,

Lafarge²,

Winckler³

et al. 2019

Algal Research

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“…In this study, these 10 objects will be named vesicles for more convenience. They were already observed in previous work [35].…”

Section: Confocal Fluorescence Microscopy Image Descriptionsupporting

confidence: 82%

“…After the use of these two methods of extraction (maceration and HPH), the B-PE content was determined by spectrophotometry. More recently, the use of high hydrostatic pressure was applied to study the extraction of B-PE [35].…”

Section: Introductionmentioning

confidence: 99%

Ex situ and in situ investigation of protein/exopolysaccharide complex in Porphyridium cruentum biomass resuspension

Tran¹,

Lafarge²,

Winckler³

et al. 2019

Algal Research

Self Cite

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“…Martinez et al (2019) employed pulsed electric field treatments (PEF, 8 or10 kV cm −1 for 150 µs) to improve the extraction efficiency of phycoerythrin [30]. Tran et al (2019) improved the extraction rate using high hydrostatic pressure. However, a pressure of 400 MPa affected the fluorescence characteristic of phycoerythrin [31].…”

Section: Discussionmentioning

confidence: 99%

“…Tran et al (2019) improved the extraction rate using high hydrostatic pressure. However, a pressure of 400 MPa affected the fluorescence characteristic of phycoerythrin [31]. In our study, the presence of other proteins reduced the purity of the crude phycoerythrin extract (A 545 /A 280 = 1.201).…”

Section: Discussionmentioning

confidence: 99%

A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Wang

et al. 2021

Foods

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The microalga Porphyridium accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of Porphyridium biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for Porphyridium; however, further purification is necessary for downstream applications.

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“…This technique, called laser scanning confocal microscopy (LSCM) [ 8 , 9 ], was originally developed for application in biology and medicine. Now, it is more and more widely used and has become an essential tool in biomedical experimental research [ 10 , 11 , 12 , 13 ]. Compared to conventional fluorescence microscopy, it overcomes the shortcomings of an out-of-focal plane-fluorescence imaging blur and focal plane glare and can improve the optical resolution of fluorescence microscopy.…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe

Sun,

Wu,

Liu

et al. 2024

Molecules

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This article reports three new two-photon absorption (TPA) materials that are quinolinium-carbazole derivates. They are 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (M4), 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (H2), and 3-(N-methyl-4-ethylquinolinium iodide)-9-ethylcarbazole (H4). Their TPA cross-sections are 491, 515, and 512 GM, respectively. Under the excitation of near-infrared light, their fluorescence emission is about 650 nm. The compounds can stain nucleic acid DNA with the same level of nuclear localization as Hoechst 33342. Under continuous irradiation with a near-infrared laser, the three new compounds showed less fluorescence decay than DAPI, and the average fluorescence decay rates were 0.016%/s, 0.020%/s, and 0.023%/s. They are expected to become new two-photon fluorescent probes of nucleic acid DNA because of their excellent performance.

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Effect of high hydrostatic pressure on extraction of B-phycoerythrin from Porphyridium cruentum: Use of confocal microscopy and image processing

Cited by 16 publications

References 45 publications

Ex situ and in situ investigation of protein/exopolysaccharide complex in Porphyridium cruentum biomass resuspension

Ex situ and in situ investigation of protein/exopolysaccharide complex in Porphyridium cruentum biomass resuspension

A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Near-Infrared Light-Excited Quinolinium-Carbazole Small Molecule as Two-Photon Fluorescence Nucleic Acid Probe

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