Effect of High Hydrostatic Pressure on Stress-Related dnaK, hrcA, and ctsR Expression Patterns in Selected Lactobacilli Strains

Bucka-Kolendo, Joanna; Juszczuk-Kubiak, Edyta; Sokołowska, Barbara

doi:10.3390/genes12111720

Cited by 8 publications

(11 citation statements)

References 29 publications

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“…As in previous studies, the HHP affects the proteome of treated LAB strains causing differences in the mass spectra profiles analyzed with MALDI-TOF MS (Bucka-Kolendo et al, 2020). Pressurization of the LAB strains can lead to changes in the expression patterns of stress-related genes (Bucka Kolendo et al, 2021). Among many functional genes associated with adaptation in LAB (Bucka-Kolendo et al, 2017), the three genes (dnaK, hrcA, and ctsR) previously described in the literature as stress-related (Bucka-Kolendo et al, 2017), were selected.…”

Section: Impact Of High Hydrostatic Pressurementioning

confidence: 99%

“…Among many functional genes associated with adaptation in LAB (Bucka-Kolendo et al, 2017), the three genes (dnaK, hrcA, and ctsR) previously described in the literature as stress-related (Bucka-Kolendo et al, 2017), were selected. The genes, like dnaK, hrcA, and ctsR, involved in the stress response (Bucka-Kolendo et al, 2021) can have different expressions under the stress factor, resulting in changes in the phenotype.…”

Section: Impact Of High Hydrostatic Pressurementioning

confidence: 99%

“…Five LAB strains (KKP 3565 Loigolactobacillus backii, KKP 3566 Loigolactobacillus backii, KKP 3568 Lactiplantibacillus plantarum, KKP 3569 Lactiplantibacillus plantarum, and KKP 3570 Lacticaseibacillus rhamnosus) were isolated from the food products, beer, tomato juice, and bread, and the process was performed according to the ISO 15214:2000 as described by Bucka-Kolendo et al (2021). In addition, bacteria were identified using genetic and proteomic methods, as defined by Bucka-Kolendo et al (2020.…”

Section: Lactic Acid Bacteriamentioning

confidence: 99%

“…LAB were grown and incubated under anaerobic conditions at 30°C for 48-72 h on MRS agar (Lactobacillus Agar DeMan, Rogosa, and Sharpe, Merc KGaA, Darmstadt, Germany), as described by Bucka-Kolendo et al (2021). For counting, plates containing less than 300 CFU/mL were selected according to ISO 4833-1:2013.…”

Section: Bacteria Growth Conditions and Count Number Analysis (Pca)mentioning

confidence: 99%

“…The total mRNA extraction and relative gene expression analysis were performed according to Bucka-Kolendo et al (2021). The custom TaqMan gene expression assays (ThermoFisher Scientific, TFS) were used for dnaK, ctsR, and hrcA.…”

Section: Bacterial Mrna Extraction and Rt-qpcr Analysismentioning

confidence: 99%

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Impact of high hydrostatic pressure on the single nucleotide polymorphism of stress-related dnaK, hrcA, and ctsR in the Lactobacillus strains

Bucka-Kolendo

Sokołowska

2022

Qual. Assur. Saf. Crops Foods

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Lactic acid bacteria (LAB) are widespread in environments and can either have a positive impact because their ability to survive in harsh conditions and influence the product (probiotic properties, change of structure-EPS [exopolysaccharides], etc.), or a negative impact, (so not needed) because of their spoilage ability (beer, juices). High hydrostatic pressure (HHP), one of the non-thermal preservation methods used in the food industry, can force the LAB to activate the adaptative mechanisms. Under pressurization, the changes in the bacteria cells can occur at the transcriptional or translational level. This study evaluated the HHP on the single nucleotide polymorphism (SNP) changes in three genes, dnaK, ctsR, and hrcA, related to the stress-response mechanism in LAB. The correlation between the DNA polymorphism and the gene expression under HHP stress was assessed. The applied pressure of 300 MPa resulted in a low ratio of nonsynonymous substitutions to the synonymous substitutions (0 to 1.12), and a lower number of mutations was observed for pressurized strains (from 6 in hrcA to 11 in dnaK) than in controlled (from 3 in ctsR to 92 in hrcA). In all pressurized strains, the expression of genes was observed, whereas, in control strains, the gene expression was detected in three out of five strains. Although there was a noticeable change in stress-related gene expression after HHP, there was no correlation with SNPs. At the same time, with a high frequency of synonymous changes in nucleotide and high diversity for hrcA and dnaK, a very low diversity was found in ctsR sequences. The LAB strains stress response mechanisms are much more complex. The study requires information on the general mechanism and changes in the membranes’ composition, proteome changes, and gene expression patterns. The mutations in genes related to stress can have important implications for the strains’ fitness effect and adaptive ability of LAB strains, especially considering their food industry implication where the HHP techniques are used.

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