The effect of surface modification of Ti-6Al-4V samples manufactured by electron beam melting (EBM) using a pulsed carbon ion beam is studied in the present work. Based on the results of XRD, SEM, and TEM analysis, patterns of changes in the microstructure and phase composition of the EBM Ti-6Al-4V alloy, depending on the number of pulses of pulsed ion beam exposure, are revealed. It was found that gradient microstructure is formed as a result of pulsed ion beam irradiation of the EBM Ti-6Al-4V samples. The microstructure of the surface layer up to 300 nm thick is represented by the (α + α”) phase. At depths of 0.3 μm, the microstructure is mixed and contains alpha-phase plates and needle-shaped martensite. The mechanical properties were investigated using methods of uniaxial tensile tests, micro- and nanohardness measurements, and tribological tests. It was shown that surface modification by a pulsed ion beam at an energy density of 1.92 J/cm2 and five pulses leads to an increase in the micro- and nanohardness of the surface layers, a decrease in the wear rate, and a slight rise in the plasticity of EBM Ti-6Al-4V alloy.