Effect of High Temperature on Pseudomonas putida NBRI0987 Biofilm Formation and Expression of Stress Sigma Factor RpoS

Srivastava, Suchi; Yadav, Archana; Seem, Karishma; Mishra, Sandhya; Chaudhary, Vidhi; Nautiyal, Chandra Shekhar

doi:10.1007/s00284-008-9105-0

Cited by 117 publications

(46 citation statements)

References 15 publications

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“…Ambient temperature has a remarkable influence on microbial biofilms. Increased ambient temperature decreases the biofilm formation of Streptococcus intermedius (1) and the rate of recovery of sonicated cultures (31) and induces bacterial stress and increased vulnerability in Pseudomonas putida (48). Increasing the temperature to 42°C led to an increase in the biofilm density in an S. epidermidis mutant derived from a clinical isolate (40).…”

Section: Discussionmentioning

confidence: 99%

Increased Temperature Enhances the Antimicrobial Effects of Daptomycin, Vancomycin, Tigecycline, Fosfomycin, and Cefamandole on Staphylococcal Biofilms

Hajdu¹,

Holinka²,

Reichmann

et al. 2010

Antimicrob Agents Chemother

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Implant-related infections are serious complications of trauma and orthopedic surgery and are most difficult to treat. The bacterial biofilms of 34 clinical Staphylococcus sp. isolates (Staphylococcus aureus, n ‫؍‬ 14; coagulase-negative staphylococci, n ‫؍‬ 19) were incubated with daptomycin (DAP; 5, 25, or 100 mg/liter), vancomycin (VAN; 5, 25, or 100 mg/liter), tigecycline (TGC; 1, 5, or 25 mg/liter), fosfomycin (FOM; 100, 250, or 1,000 mg/liter), and cefamandole (FAM; 50, 100, or 500 mg/liter) for 24 h at three different ambient temperatures: 35°C, 40°C, and 45°C. To quantify the reduction of the biomass, the optical density ratio (ODr) of stained biofilms and the number of growing bacteria were determined. Increasing the temperature to 45°C or to 40°C during incubation with FAM, FOM, TGC, VAN, or DAP led to a significant but differential reduction of the thickness of the staphylococcal biofilms compared to that at 35°C (P < 0.05). Growth reduction was enhanced for DAP at 100 mg/liter at 35°C, 40°C, and 45°C (log count reductions, 4, 3.6, and 3.3, respectively; P < 0.05). A growth reduction by 2 log counts was detected for FAM at a concentration of 500 mg/liter at 40°C and 45°C (P ‫؍‬ 0.01). FOM at 1,000 mg/liter reduced the bacterial growth by 1.2 log counts (not significant). The antibacterial activity of antimicrobial agents is significantly but differentially enhanced by increasing the ambient temperature and using high concentrations. Adjuvant hyperthermia may be of value in the treatment of biofilm-associated implant-related infections.

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Section: Discussionmentioning

confidence: 99%

Increased Temperature Enhances the Antimicrobial Effects of Daptomycin, Vancomycin, Tigecycline, Fosfomycin, and Cefamandole on Staphylococcal Biofilms

Hajdu¹,

Holinka²,

Reichmann

et al. 2010

Antimicrob Agents Chemother

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“…Biofilm formation by microorganisms of the genus Pseudomonas is associated with various stress factors such as oxidative stress 18) , high temperature 25) , hydrodynamic stress 23) and small amounts of ethanol or antibiotic 6,7,10) . The biofilm formation of S. mutans also increases by various stress factors such as oxidative stress, nutritional stress and acid circumstance 21) .…”

Section: Discussionmentioning

confidence: 99%

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Hirai

Tomida

Kikuchi

et al. 2010

Bull. Tokyo Dent. Coll.

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A complex aggregation of microorganisms growing on a solid substrate is termed a biofilm and is considered to be an etiological agents. Pseudomonas aeruginosa and Streptococcus mutans are representative bacteria in such biofilms. It is well known that deuterium oxide (D 2 O) causes toxic effects on a number of biological systems. We investigated the effects of D 2 O on growth and biofilm formation of P. aeruginosa and S. mutans. These bacteria were incubated in medium containing D 2 O (100%, 75% or 0%) at 37°C for 24hr, 48 hr or 72hr. Growth of P. aeruginosa was inhibited by D 2 O within the first 48hr. However, after 72 hr, growth rate was seen to increase in the D 2 O-containing medium compared with in medium without D 2 O. In contrast, the growth of S. mutans in the D 2 O medium was inhibited within 72hr. The biofilm formation of P. aeruginosa was increased in the D 2 O medium. Biofilm formation of S. mutans in the D 2 O medium increased compared with in the medium without D 2 O, but this increase was only temporary in the case of P. aeruginosa. Compared to biofilm formation in 0% D 2 O medium marked as 100%, the biofilm formation rate of S. mutans in 75% D 2 O medium was 143% at 24hr, 146% at 48 hr and 130% at 72 hr. In other D 2 O concentration media biofilm formation was lower. In 100% D 2 O medium, biofilm formation rate decreased from 114% at 24hr to 56% at 72hr. The biofilm formation rate of P. aeruginosa in 100% D 2 O medium was 172% at 24hr, but decreased to 88% at 72hr. Biofilm formation of P. aeruginosa in 75% and 0% D 2 O media showed no significant difference. We consider that these results were due to stress or alteration in bacterial metabolisms.

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“…Many studies have reported the efficiency of plant growth promoting rhizobacteria (PGPR) under determined conditions, in protecting plants from the deleterious effects of environmental stresses (Enebak et al 1997;Glick et al 1997;Timmusk and Wagner 1999). Recently, rhizosphere and endorhizosphere microorganisms are reported to help plants tolerate abiotic stresses by a variety of mechanisms including modification of plant response at gene level (Bashan and Holguin 1998;Srivastava et al 2008). PGPR are adapted to adverse conditions and may compensate for such detrimental conditions (Marulanda et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress

et al. 2010

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Drought is one of the major abiotic stresses affecting yield of dryland crops. Rhizobacterial populations of stressed soils are adapted and tolerant to stress and can be screened for isolation of efficient stress adaptive/tolerant, plant growth promoting rhizobacterial (PGPR) strains that can be used as inoculants for crops grown in stressed ecosystems. The effect of inoculation of five drought tolerant plant growth promoting Pseudomonas spp. strains namely P. entomophila strain BV-P13, P. stutzeri strain GRFHAP-P14, P. putida strain GAP-P45, P. syringae strain GRFHYTP52, and P. monteilli strain WAPP53 on growth, osmoregulation and antioxidant status of maize seedlings under drought stress conditions was investigated. Drought stress induced by withholding irrigation had drastic effects on growth of maize seedlings. However seed bacterization of maize with Pseudomonas spp. strains improved plant biomass, relative water content, leaf water potential, root adhering soil/root tissue ratio, aggregate stability and mean weight diameter and decreased leaf water loss. The inoculated plants showed higher levels of proline, sugars, free amino acids under drought stress. However protein and starch content was reduced under drought stress conditions. Inoculation decreased electrolyte leakage compared to uninoculated seedlings under drought stress. As compared to uninoculated seedlings, inoculated seedlings showed significantly lower activities of antioxidant enzymes, ascorbate peroxidase (APX), catalase (CAT), glutathione peroxidase (GPX) under drought stress, indicating that inoculated seedlings felt less stress as compared to uninoculated seedlings. The strain GAP-P45 was found to be the best in terms of influencing growth and biochemical and physiological status of the seedlings under drought stress. The study reports the potential of rhizobacteria in alleviating drought stress effects in maize.

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Effect of High Temperature on Pseudomonas putida NBRI0987 Biofilm Formation and Expression of Stress Sigma Factor RpoS

Cited by 117 publications

References 15 publications

Increased Temperature Enhances the Antimicrobial Effects of Daptomycin, Vancomycin, Tigecycline, Fosfomycin, and Cefamandole on Staphylococcal Biofilms

Increased Temperature Enhances the Antimicrobial Effects of Daptomycin, Vancomycin, Tigecycline, Fosfomycin, and Cefamandole on Staphylococcal Biofilms

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress

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