Effect of high temperature stress on heat shock protein expression and antioxidant enzyme activity of two morphs of the mud crab Scylla paramamosain

Liu, Zi-Ming; Xianlong, Zhu; Lu, Jun; Cai, Wan-Jun; Ye, Yaping; Lv, Yao-Ping

doi:10.1016/j.cbpa.2018.04.016

Cited by 45 publications

(21 citation statements)

References 48 publications

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“…There is considerable evidence that HSPs are essential for plant survival under abiotic stresses [ 56 , 57 ]. The expression of HSP genes is observed to be induced by high temperatures and inhibited by low temperatures [ 58 , 59 ]. For example, CRPs are induced to enable plants to survive from chilling and freezing stress [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Chen

et al. 2021

Molecules

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Hypericin (Hyp), well-known as an antidepressant, is mainly extracted from Hypericum perforatum. Although Hyp accumulation and biomass are greater at lower compared to higher temperature, the regulation mechanism has not been reported. Here, the physiological characteristics and transcriptome of H. perforatum grown at 15 and 22 °C were determined and analyzed by HPLC and de novo sequencing. The results showed that the stomatal density and opening percentages were 1.1- and 1.4-fold more, and the Hyp content was 4.5-fold greater at 15 °C compared to 22 °C. A total of 1584 differentially expressed genes (DEGs) were observed at 15 versus 22 °C, with 749 characterized genes, 421 upregulated (UR) and 328 downregulated (DR). Based on biological functions, 150 genes were associated with Hyp biosynthesis, plant growth and the stress response, including photosynthesis, carbohydrate metabolism, fatty acids metabolism, cytochrome P450 (CYPs), morpho-physiological traits, heat shock proteins (HSPs), cold-responsive proteins (CRPs) and transcription factors (TFs). The differential expression levels of the master genes were confirmed by qRT-PCR and almost consistent with their Reads Per kb per Million (RPKM) values. This physiological and transcriptomic analyses provided insight into the regulation mechanisms of low temperature enhancing Hyp biosynthesis in H. perforatum.

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Section: Discussionmentioning

confidence: 99%

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Chen

et al. 2021

Molecules

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“…Environmental stress has an influence on many biological functions and disturbs homeostasis [ 1 ]. Temperature is one of the most important environmental factors which can affect the food intake, growth, maturation, reproduction, behavior, physiology, metabolism and even survival of economic teleosts [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Appropriate temperatures may promote the growth of fish, while temperatures above the optimum boundary will have negative impacts [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

Yan

Qiao

et al. 2022

IJMS

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As an economically important flatfish in Asia, Japanese flounder is threatened by continuously rising temperatures due to global warming. To understand the molecular responses of this species to temperature stress, adult Japanese flounder individuals were treated with two kinds of heat stress—a gradual temperature rise (GTR) and an abrupt temperature rise (ATR)—in aquaria under experimental conditions. Changes in histopathology, programmed cell death levels and the oxidative stress status of gills were investigated. Histopathology showed that the damage caused by ATR stress was more serious. TUNEL signals confirmed this result, showing more programmed cell death in the ATR group. In addition, reactive oxygen species (ROS) levels and the 8-O-hDG contents of both the GTR and ATR groups increased significantly, and the total superoxide dismutase (T-SOD) activities and total antioxidant capacity (T-AOC) levels decreased in the two stressed groups, which showed damage to antioxidant systems. Meanwhile, RNA-seq was utilized to illustrate the molecular mechanisms underyling gill damage. Compared to the control group of 18 °C, 507 differentially expressed genes (DEGs) were screened in the GTR group; 341 were up-regulated and 166 were down-regulated, and pathway enrichment analysis indicated that they were involved in regulation and adaptation, including chaperone and folding catalyst pathways, the mitogen-activated protein kinase signaling (MAPK) pathway and DNA replication protein pathways. After ATR stress, 1070 DEGs were identified, 627 were up-regulated and 423 were down-regulated, and most DEGs were involved in chaperone and folding catalyst and DNA-related pathways, such as DNA replication proteins and nucleotide excision repair. The annotation of DEGs showed the great importance of heat shock proteins (HSPs) in protecting Japanese flounder from heat stress injury; 12 hsp genes were found after GTR, while 5 hsp genes were found after ATR. In summary, our study records gill dysfunction after heat stress, with different response patterns observed in the two experimental designs; chaperones were activated to defend heat stress after GTR, while replication was almost abandoned due to the severe damage consequent on ATR stress.

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“…The coastal waters where pearl oysters live have undergone unprecedented changes, especially MHWs posing a great threat to the survival of P. maxima. Although studies have been conducted to evaluate the effects of temperature on the expression level of HSP genes in some marine invertebrates (Park et al, 2015;Chen et al, 2018;Liu et al, 2018;Zhao et al, 2020), little is known about thermal resistant gene expression responses of the P. maxima to MHWs. Therefore, it is particularly important to explore the molecular mechanism of pearl oysters' adaptability to MHWs.…”

Section: Introductionmentioning

confidence: 99%

Responses of Pearl Oysters to Marine Heatwaves as Indicated by HSP70

Liang

et al. 2022

Front. Mar. Sci.

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Marine heatwaves (MHWs) can severely affect bivalves and ecosystems they support. Heat shock proteins (HSPs) are a group of molecular chaperones playing a critical role in the cellular protection and thermo tolerance and thereby constraining physiological responses of marine bivalves to MHWs. Here, we cloned the full-length of HSP70 cDNA from the Pinctada maximal (PmHSP70) and evaluated the expression of PmHSP70 in pearl oysters under acute and repeatedly occurring MHWs conditions. The full-length of PmHSP70 is 2,474 bp, containing an ORF of 1,956 bp encoding 655 amino acids with a predicted molecular weight of 71.23 kDa and 5.26 theoretical isoelectric point. Under the scenario of acute MHWs, the expression of PmHSP70 was significantly highly expressed at 32 and 36°C, and reached the highest at 12 and 72 h, respectively, indicating that pearl oysters rapidly up-regulated the expression of HSP70 in response to MHWs. In the repeatedly occurring MHWs scenario, the thermal response of pearl oysters was alleviated, as best exemplified by significantly lowered expression levels of PmHSP70. Therefore, we speculate that long-term and repeated MHWs can alleviate the thermal stress of pearl oysters. This finding is encouraging and will provide us with meaningful insights into the acclimation of marine bivalves to extreme environments in the future.

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Effect of high temperature stress on heat shock protein expression and antioxidant enzyme activity of two morphs of the mud crab Scylla paramamosain

Cited by 45 publications

References 48 publications

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in Hypericum perforatum

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

Responses of Pearl Oysters to Marine Heatwaves as Indicated by HSP70

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