Effect of high versus low doses of fat and vitamin A dietary supplementation on fatty acid composition of phospholipids in mice

Weiss, Kathrin; Mihály, Johanna; Liebisch, Gerhard; Marosvölgyi, Tamás; García, Ada L.; Schmitz, Gerd; Décsi, Támas; Rühl, Ralph

doi:10.1007/s12263-013-0368-0

Cited by 12 publications

(19 citation statements)

References 35 publications

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“…Thus, the upregulation of genes involving in VA metabolism in high-FE pigs was possibly caused by the relatively lower oxidation and energy loss in this study. Besides, the key genes of fatty acid biosynthesis SCD (Samuel et al 2001; Zolfaghari and Ross 2003; Zhang et al 2012; Weiss et al 2014) and FASN (Zhou et al 2010; Zhang et al 2012) were induced by RA at the transcriptional level and significantly upregulated in high-FE pigs. SCD encodes stearoyl-CoA desaturase, which converts saturated fatty acids into monounsaturated fatty acids, and influences fatty acid partitioning in the liver by promoting fatty acid synthesis but decreasing oxidation (Flowers and Ntambi 2009).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals that Vitamin A Metabolism in the Liver Affects Feed Efficiency in Pigs

Zhao

Hou

Liu

et al. 2016

G3 Genes|Genomes|Genetics

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Feed efficiency (FE) is essential for pig production. In this study, 300 significantly differentially expressed (DE) transcripts, including 232 annotated genes, 28 cis-natural antisense transcripts (cis-NATs), and 40 long noncoding RNAs (lncRNAs), were identified between the liver of Yorkshire pigs with extremely high and low FE. Among these transcripts, 25 DE lncRNAs were significantly correlated with 125 DE annotated genes at a transcriptional level. These DE genes were enriched primarily in vitamin A (VA), fatty acid, and steroid hormone metabolism. VA metabolism is regulated by energy status, and active derivatives of VA metabolism can regulate fatty acid and steroid hormones metabolism. The key genes of VA metabolism (CYP1A1, ALDH1A2, and RDH16), fatty acid biosynthesis (FASN, SCD, CYP2J2, and ANKRD23), and steroid hormone metabolism (CYP1A1, HSD17B2, and UGT2B4) were significantly upregulated in the liver of high-FE pigs. Previous study with the same samples indicated that the mitochondrial function and energy expenditure were reduced in the muscle tissue of high-FE pigs. In conclusion, VA metabolism in liver tissues plays important roles in the regulation of FE in pigs by affecting energy metabolism, which may mediate fatty acid biosynthesis and steroid hormone metabolism. Furthermore, our results identified novel transcripts, such as cis-NATs and lncRNAs, which are also involved in the regulation of FE in pigs.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals that Vitamin A Metabolism in the Liver Affects Feed Efficiency in Pigs

Zhao

Hou

Liu

et al. 2016

G3 Genes|Genomes|Genetics

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“…On the basis of the analyzed feed of the NF diet, it contained 11.6% saturated fats, 20% monounsaturated fatty acids, and 68.4% polyunsaturated fatty acids (Weiss et al, in preparation). Furthermore, dietary composition was 180 g/kg casein, 10 g/kg vitamin mix, 45 g/kg mineral mix, and 20 g/kg cellulose for all applied diets (42). As a result of the increased amount of fat in the diet, the carbohydrate proportion was lower; the LF diet contained 29.5% sucrose and 43% starch, the NF diet 28% sucrose and 41.5% starch, and the HF diet 17% sucrose and 32.5% starch (42).…”

Section: Methodsmentioning

confidence: 99%

“…For vitamin content, diets were supplemented with vitamin mix (Vitamin-Vormischung C1000) that contained either 2500 RE/kg as normal–vitamin A diet, or for high–vitamin A diet, an additional retinyl-palmitate (RetPal) supplement (final 326,500 RE/kg; Sigma-Aldrich) was added to the normal–vitamin A diet (42, 43). …”

Section: Methodsmentioning

confidence: 99%

Reduced adiponectin expression after high‐fat diet is associated with selective up‐regulation of ALDH1A1 and further retinoic acid receptor signaling in adipose tissue

et al. 2016

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Adiponectin is an adipocyte-derived adipokine with potent antidiabetic, anti-inflammatory, and antiatherogenic activity. Long-term, high-fat diet results in gain of body weight, adiposity, further inflammatory-based cardiovascular diseases, and reduced adiponectin secretion. Vitamin A derivatives/retinoids are involved in several of these processes, which mainly take place in white adipose tissue (WAT). In this study, we examined adiponectin expression as a function of dietary high-fat and high–vitamin A conditions in mice. A decrease of adiponectin expression in addition to an up-regulation of aldehyde dehydrogenase A1 (ALDH1A1), retinoid signaling, and retinoic acid response element signaling was selectively observed in WAT of mice fed a normal–vitamin A, high-fat diet. Reduced adiponectin expression in WAT was also observed in mice fed a high–vitamin A diet. Adipocyte cell culture revealed that endogenous and synthetic retinoic acid receptor (RAR)α- and RARγ-selective agonists, as well as a synthetic retinoid X receptor agonist, efficiently reduced adiponectin expression, whereas ALDH1A1 expression only increased with RAR agonists. We conclude that reduced adiponectin expression under high-fat dietary conditions is dependent on 1) increased ALDH1A1 expression in adipocytes, which does not increase all-trans-retinoic acid levels; 2) further RAR ligand–induced, WAT-selective, increased retinoic acid response element–mediated signaling; and 3) RAR ligand–dependent reduction of adiponectin expression.—Landrier, J.-F., Kasiri, E., Karkeni, E., Mihály, J., Béke, G., Weiss, K., Lucas, R., Aydemir, G., Salles, J., Walrand, S., de Lera, A. R., Rühl, R. Reduced adiponectin expression after high-fat diet is associated with selective up-regulation of ALDH1A1 and further retinoic acid receptor signaling in adipose tissue.

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“…Our previous studies in mice have shown that diet modulates the hepatic gene expression of SCD1. A diet rich in sunflower oil, and its major fatty acid constituents (n6-PUFAs) was an effective suppressor of the hepatic SCD1 and ELOVL6 expression [22]. This suppression was also observed in phospholipid composition concomitantly with a significant reduction of their metabolic products measured in cellular membranes of liver tissue, in particular MUFAs [22].…”

Section: Introductionmentioning

confidence: 91%

“…A diet rich in sunflower oil, and its major fatty acid constituents (n6-PUFAs) was an effective suppressor of the hepatic SCD1 and ELOVL6 expression [22]. This suppression was also observed in phospholipid composition concomitantly with a significant reduction of their metabolic products measured in cellular membranes of liver tissue, in particular MUFAs [22]. The mechanism of suppression of SCD1 and ELOVL6 by dietary fats with a high n6-PUFA content is still not fully understood, while it is well known that dietary n3-PUFAs also have a pronounced suppressive effect on the expression of these enzymes [23].…”

Section: Introductionmentioning

confidence: 99%

Saturated and monounsaturated fatty acids in membranes are determined by the gene expression of their metabolizing enzymes SCD1 and ELOVL6 regulated by the intake of dietary fat

et al. 2019

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Purpose We investigated the effect of dietary fats on the incorporation of saturated (SAFAs) and monounsaturated dietary fatty acids (MUFAs) into plasma phospholipids and the regulation of the expression of lipid-metabolizing enzymes in the liver. Methods Mice were fed different diets containing commonly used dietary fats/oils (coconut fat, margarine, fish oil, sunflower oil, or olive oil) for 4 weeks (n = 6 per diet group). In a second experiment, mice (n = 6 per group) were treated for 7 days with synthetic ligands to activate specific nuclear hormone receptors (NHRs) and the hepatic gene expression of CYP26A1 was investigated. Hepatic gene expression of stearoyl-coenzyme A desaturase 1 (SCD1), elongase 6 (ELOVL6), and CYP26A1 was examined using quantitative real-time PCR (QRT-PCR). Fatty acid composition in mouse plasma phospholipids was analyzed by gas chromatography (GC). Results We found significantly reduced hepatic gene expression of SCD1 and ELOVL6 after the fish oil diet compared with the other diets. This resulted in reduced enzyme-specific fatty acid ratios, e.g., 18:1n9/18:0 for SCD1 and 18:0/16:0 and 18:1n7/16:1n7 for ELOVL6 in plasma phospholipids. Furthermore, CYP26A1 a retinoic acid receptor-specific target was revealed as a new player mediating the suppressive effect of fish oil-supplemented diet on SCD1 and ELOVL6 hepatic gene expression. Conclusion Plasma levels of MUFAs and SAFAs strongly reflect an altered hepatic fatty acid-metabolizing enzyme expression after supplementation with different dietary fats/oils.

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Effect of high versus low doses of fat and vitamin A dietary supplementation on fatty acid composition of phospholipids in mice

Cited by 12 publications

References 35 publications

Transcriptome Analysis Reveals that Vitamin A Metabolism in the Liver Affects Feed Efficiency in Pigs

Transcriptome Analysis Reveals that Vitamin A Metabolism in the Liver Affects Feed Efficiency in Pigs

Reduced adiponectin expression after high‐fat diet is associated with selective up‐regulation of ALDH1A1 and further retinoic acid receptor signaling in adipose tissue

Saturated and monounsaturated fatty acids in membranes are determined by the gene expression of their metabolizing enzymes SCD1 and ELOVL6 regulated by the intake of dietary fat

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