Effect of hirudin on platelet deposition to an artificial surface during low-stress shear flow of whole blood

Alkhamis, Taha M.; Beissinger, Richard L.; Chediak, Juan

doi:10.1016/0142-9612(93)90009-q

Cited by 19 publications

(19 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Ca 2ϩ concentration has been shown to figure prominently in the potency of small glycoprotein IIb/IIIa antagonists in platelet aggregation in platelet-rich plasma (Marciniak et al, 2001), our data indicate that Ca 2ϩ ions have little if any bearing on shearinduced platelet activation and adhesion. Another report, however, showed that blood in hirudin anticoagulant reduced (up to 50%) platelet adhesion under shear stress (Alkhamis et al, 1993). This difference may reflect the use of different systems, i.e., the use of 5680-s Ϫ1 shear rate in the previous study versus 1875-s Ϫ1 shear rate in our current article, as well as the use of different platelet adhesion plate surfaces, i.e., tetrafluoroethylene-propylene copolymer versus polystyrene in our study.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Various Platelet Glycoprotein IIb/IIIa Antagonists on Shear-Induced Platelet Activation and Adhesion

Wang

Dorsam

Lauver

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Platelet accretion into arterial thrombus in stenotic arterial vessels involves shear-induced platelet activation and adhesion. The Cone and Plate(let) Analyzer (CPA) is designed to simulate such conditions in vitro under a rotating high shear rate in whole blood. In the present study, we evaluated various experimental conditions (including aspirin, temperature, and calcium concentration) and investigated the effects of small molecules along with peptide glycoprotein IIb/IIIa antagonists on platelet adhesion using the CPA system. Concentration-dependent effect of glycoprotein IIb/IIIa antagonists on shear-induced platelet adhesion showed marked differences in potencies: IC 50 ϭ 34, 35, 91, 438, and 606 nM for DPC802 (a specific glycoprotein IIb/IIIa antagonist), roxifiban, sibrafiban, lotrafiban, and orbofiban (free acid forms), respectively, and IC 50 values of 43, 430, and 5781 nM for abciximab, tirofiban, and eptifibatide, respectively. Parallel study was also conducted to evaluate the effect of glycoprotein IIb/IIIa inhibitors using optical aggregometry. The potency of fibans in blocking shear-induced platelet adhesion correlated well with their binding affinity to the resting and activated glycoprotein IIb/IIIa receptors, as well as their "off-rates". Nevertheless, none of these fibans was able to effectively block shear-induced platelet adhesion at targeted clinical dosing regimens except for abciximab. These data suggest that glycoprotein IIb/IIIa antagonists that show similar efficacy in the inhibition of platelet aggregation in a static in vitro assay may differ substantially in a shear-based system of platelet adhesion. The clinical significance of this phenomenon awaits further investigation.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Various Platelet Glycoprotein IIb/IIIa Antagonists on Shear-Induced Platelet Activation and Adhesion

Wang

Dorsam

Lauver

et al. 2002

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

“…Several authors have investigated the role of red blood cells in platelet activation and adhesion. Alkhamis and coworkers established that red blood cells release a significant fraction of ADP which is enough to induce platelet aggregation and adhesion [29]. Valles et al [30] demonstrated that interactions between red blood cells and platelets amplify platelet reactivity.…”

Section: Discussionmentioning

confidence: 99%

Influence of nanoporesize on platelet adhesion and activation

Ferraz

Carlsson

Hong

et al. 2008

J Mater Sci: Mater Med

View full text Add to dashboard Cite

In this study we have evaluated the influence of biomaterial nano-topography on platelet adhesion and activation. Nano-porous alumina membranes with pore diameters of 20 and 200 nm were incubated with whole blood and platelet rich plasma. Platelet number, adhesion and activation were determined by using a coulter hematology analyzer, scanning electron microscopy, immunocytochemical staining in combination with light microscopy and by enzyme immunoassay. Special attention was paid to cell morphology, microparticle generation, Pselectin expression and b-TG production. Very few platelets were found on the 200 nm alumina as compared to the 20 nm membrane. The platelets found on the 20 nm membrane showed signs of activation such as spread morphology and protruding filipodia as well as P-selectin expression. However no microparticles were detected on this surface. Despite the fact that very few platelets were found on the 200 nm alumina in contrast to the 20 nm membrane many microparticles were detected on this surface. Interestingly, all microparticles were found inside circular shaped areas of approximately 3 lm in diameter. Since this is the approximate size of a platelet we speculate that this is evidence of transient, non-adherent platelet contact with the surface, which has triggered platelet microparticle generation. To the authors knowledge, this is the first study that demonstrates how nanotexture can influence platelet microparticle generation. The study highlights the importance of understanding molecular and cellular events on nano-level when designing new biomaterials.

show abstract

“…However, the small ratio of test surface to total wetted surface by contact with blood (<40%) constitutes a handicap. The same limitation results if viscometers are used: the percentage of relevant surface is low (<50%). Furthermore, it is impossible to run parallel tests simultaneously without the use of several viscometers.…”

Section: Introductionmentioning

confidence: 94%

A novel blood incubation system for the in‐vitro assessment of interactions between platelets and biomaterial surfaces under dynamic flow conditions: The Hemocoater

Boudot

Boccoz

Düregger

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

Hemocompatibility evaluation of biomaterials necessitates the use of blood incubation systems which simulate physiological flow conditions. However, most of the current systems have various limitations, especially restricted material variability, poor access to the test surface or damage of blood cells due to the use of a pump. In this paper, we combined the advantages of existent setups and developed a new planar shaped incubation test bench to lift those restrictions and mimic the pulsatile in-vivo situation. The adjustable flow conditions at the tested material surface were defined and corresponded to those in blood vessels. Platelet/material-interaction, as major aspect of hemocompatibility, was investigated for four common polymeric materials (polyoxymethylene, polypropylene, polyethylene and silicone elastomer) with platelet deprivation and platelet adhesion tests. Highly significant differences in the adhesion of platelets onto the tested material surfaces were measured. The number of adhered platelets on the most hydrophobic sample (silicone elastomer) was four-times higher than on the most hydrophilic sample (polyoxymethylene). These findings were confirmed with a scanning microscopic analysis and demonstrated the suitability of the testing device for the evaluation of platelet/material interactions. Moreover, hemolysis measurements demonstrated that the system did not provoke blood damage. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2430-2440, 2016.

show abstract

Effect of hirudin on platelet deposition to an artificial surface during low-stress shear flow of whole blood

Cited by 19 publications

References 20 publications

Comparative Analysis of Various Platelet Glycoprotein IIb/IIIa Antagonists on Shear-Induced Platelet Activation and Adhesion

Comparative Analysis of Various Platelet Glycoprotein IIb/IIIa Antagonists on Shear-Induced Platelet Activation and Adhesion

Influence of nanoporesize on platelet adhesion and activation

A novel blood incubation system for the in‐vitro assessment of interactions between platelets and biomaterial surfaces under dynamic flow conditions: The Hemocoater

Contact Info

Product

Resources

About