Effect of histamine and the H₂ antagonist cimetidine on the growth and migration of human neoplastic gila

Abstract

Histamine is known to act, at least in part, as a growth factor, as production of this neurotransmitter has been found to accelerate the rate of tissue proliferation in wound repair, embryogenesis and malignant growth. Histamine favours in vivo tumour cell proliferation via H2 receptors. Cimetidine is an H2 blocker and has been shown to inhibit tumour cell growth. In the present study, the growth modulating effects of histamine and cimetidine were assessed on five cell lines derived from human brain tumours of different histological types and grades of malignancy. Each cell line was treated with either cimetidine or histamine for 24 h before kinetic analyses, with PCNA, or motility assays, using Transwell migration chambers incorporating a microporous membrane, were carried out. Cimetidine significantly inhibited cell proliferation in three out of the five cell lines, which may indicate the dependence of proliferation of these cell lines on stimulation of the H2 receptor. With regard to migration, it was observed that in the majority of cell lines, cimetidine induced migration whilst histamine inhibited it. It was concluded that the link between effects of histamine on proliferation and its effects on migration must be clarified using a larger sample of cell lines.

Cimetidine, an unexpected anti-tumor agent, and its potential for the treatment of glioblastoma (review)

“…Van der Ven and colleagues (99) and Finn and colleagues (100) had previously tested the growth-modulating effects of CIM on glioma cultures derived from human brain tumors. They observed that high dose (1 mM) CIM induced inhibition of in vitro proliferation of gliomas, while lower concentrations (1 μM) were less effective (99,100). We observed that in vitro 0.1-1 μM CIM significantly decreased migration of both U373 and 9L brain tumor cells (21).…”

Byrsonima fagifolia: An integrative study to validate the gastroprotective, healing, antidiarrheal, antimicrobial and mutagenic action

“…Morphological analysis of the slides submitted to PCNA (Fig. 3) show that cimetidine significantly inhibited cell proliferation in three out of the five cell lines, which may indicate the dependence of proliferation of these cell lines on stim- Table 4 Effects of the treatment for 14 consecutive days with methanolic extract from Byrsonima fagifolia-BF (500 mg/kg) or cimetidine (100 mg/kg) on healing of ulcer produced by injecting acetic acid solution into the stomachs of rats ulation of the H 2 receptor (Finn et al, 1996). In the BF group there are increases of cell proliferation in the base of mucosa glands in the lesion region (Fig.…”

Effect of Mouriri pusa tannins and flavonoids on prevention and treatment against experimental gastric ulcer

“…It is mainly expressed during S phase of cell cycle, hence, in dividing cells (Celis and Celis, 1985). Finn et al (1996) showed that cimetidine significantly inhibited the proliferation of three of five cell lines, what my indicate dependence by these lines on H 2 receptors activation. Flavonoids fraction presented significant augment on PCNA positive cells comparing to Saline (Table 1), indicating greater cell proliferation in the regenerative …”