Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-α production stimulated by LPS

Han, Sang‐Mi; Lee, Kwang-Gill; Yeo, Joo‐Hong; Kweon, HaeYong; Woo, Soon-Ok; Lee, Myeong-lyeol; Baek, Ha-Ju; Sun-Yeou, Kim; Park, Kwan‐Kyu

doi:10.1016/j.jep.2006.11.008

Cited by 56 publications

(36 citation statements)

References 39 publications

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“…In addition, recent studies reported several effects of bee venom such as induction of apoptosis and necrosis, and effects on proliferation, cytotoxicity, and growth inhibition of different types of cancer cells (6)(7)(8)(9)(10)(11).…”

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confidence: 99%

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Garaj-Vrhovac

Gajski

2009

Archives of Industrial Hygiene and Toxicology

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Several studies have reported radioprotective, antimutagenic, anti-infl ammatory, antinociceptive, and anticancer effects of bee venom both in the cell and the whole organism. The aim of this study was to assess the effects of a single high dose of 100 µg mL -1 of whole bee venom in human lymphocytes in vitro over a variety of time spans (from 10 min to 24 h). After the treatment, we used the comet assay and micronucleus test to see the effect of bee venom on the cell. The comet assay confi rmed that the venom damaged the DNA molecule. Tail length, tail intensity, tail moment showed a signifi cant increase (P<0.05). The percentage of long-tailed nuclei (LTN) with the tail length exceeding the 95 th percentile also increased in a time-dependent manner. The micronucleus parameters (number of micronuclei, nucleoplasmic bridges, and nuclear buds) also showed a signifi cant time-dependent increase (P<0.05). This research indicates that high concentrations of bee venom can lead to cellular instability. Further research is needed to understand the mechanism of action of bee venom and its components in human cells and to see if this natural product may fi nd application in medicine.

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confidence: 99%

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Garaj-Vrhovac

Gajski

2009

Archives of Industrial Hygiene and Toxicology

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“…For example, BV inhibited the expression of specific inflammatory genes that were upregulated by nuclear factor-κB in the presence of LPS, including mitogen-activated protein kinase 8 (MAP3K8), TNF, TNF-α-induced protein 3 (TNFAIP3), suppressor of cytokine signaling 3 (SOCS3), TNF receptor-associated factor 1 (TRAF1), juN and CREB binding protein (CBP). However, it should be noted that BV could inhibit both proand anti-inflammatory cytokine expression (4,30). It may be noteworthy to identify certain biological compounds in BV that are responsible for altering transcription expression of both inflammatory cytokines and the mechanism whereby BV induces growth arrest in different phases in cervical cells.…”

Section: Discussionmentioning

confidence: 99%

Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line

et al. 2015

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Abstract. Bee venom (BV) therapy is a type of alternative medical treatment used to treat various diseases in oriental medicine. The mechanisms underlying the effects of BV remain poorly understood. In the present study, we evaluated the antiviral effect of BV on cervical carcinoma cell lines (CaSki, HeLa, C33A and TC-1). BV treatments resulted in a more significant suppression of cell growth in HPV 16-infected cells (CaSki) and a lesser suppression in HPV 18-infected cells (HeLa). However, less suppression was observed in HPV-negative C33A cells. In 10 µg/ml BV-treated CaSki cells, the mRNA expression and protein levels of HPV16 E6 and E7 were significantly decreased by BV, while HPV18 E6 and E7 mRNA expression levels were not significantly altered by 10 µg/ml BV-treated HeLa cells. The antitumor effects of BV were in accordance with in vitro data, in restricting tumor growth in vivo and were much more effective on the suppression of tumor growth. Furthermore, the mRNA and protein expression levels of HPV16 E6 and E7 were decreased by BV in TC-1 tumors. These findings demonstrated the antiviral effects of BV in HPV-infected cervical cancer cells and the anticancer effects of BV in HPV16 E6/E7-expressed TC-1 tumors. Collectively, BV plays a differential role in suppressing HPV16-infected cells (CaSki cells) and HPV18-infected cells (HeLa cells) by the downregulation of E6/E7 protein of HPV16/18.

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“…The BV was lyophilized by freeze drying and stored. 8) Animals and Experimental Protocol Female BALB/c mice, which were six-weeks old and free of murine specific pathogens, were obtained from Hyosung Science (Daegu, South Korea). They were maintained under standard laboratory conditions in a pathogen-free cage.…”

Section: Methodsmentioning

confidence: 99%

Anti-allergic Effect of Bee Venom in an Allergic Rhinitis Mouse Model

Shin

Kim

et al. 2014

Biological & Pharmaceutical Bulletin

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Bee venom (BV) has been used as an anti-inflammatory and immune modulating agent in Oriental medicine. This study used a mouse model to investigate the anti-allergic effect of BV, which is used in the treatment of various inflammatory diseases in traditional medicine. BV was obtained from the National Institute of Agricultural Science and Technology of Korea. Female BALB/C mice were sensitized by intraperitoneal injection of ovalbumin (OVA). BV was administered nasally prior to the intranasal instillation of OVA. Allergic behavior, serum OVA-specific immunoglobulin E (IgE), interleukin (IL)-4, IL-10, and interferon-gamma (INF-γ) levels in nasal lavage fluid were measured. Hematoxylin-eosin and periodic acid-Schiff staining were performed to evaluate histological change. BV attenuated nasal symptoms and inhibited the production of OVA-specific IgE and IL-4 in sensitized mice. The degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by BV. Thus, BV effectively reduced allergic inflammation in a mouse model of allergic rhinitis, suggesting its potential as a useful therapeutic agent to treat allergic rhinitis.Key words bee venom; allergic rhinitis; ovalbumin; mouse model Allergic rhinitis (AR) is characterized by nasal mucosal inflammation resulting from immunoglobulin E (IgE) mediated hypersensitivity reaction. Allergen exposures stimulate infiltration of inflammatory cells within the nasal mucosa, including basophils, eosinophils, mast cells, and mononuclear cells. These inflammatory cells release several allergic mediators, such as histamine, cysteinyl leukotrienes, and prostaglandins, which sustain the inflammatory reaction and produce characteristic nasal symptoms of, sneezing, itching, rhinorrhea and nasal congestion. Animal models of the allergic response to inhaled allergens have been studied to elucidate the mechanisms leading to the development of inflammation and the therapeutic effect of newly developed anti-inflammatory agents. Repeated exposure of mice to ovalbumin (OVA) has been used to develop an allergic model with inflammatory cell infiltration and increased thickness of the epithelial layer. 1)Bee venom (BV) consists of a various biologically active amines, peptides and nonpeptide components, and has radioprotective, antimutagenic, anti-inflammatory, antinociceptive, and anticancer activities.2,3) Two main components of BV, melittin and adolapin, have anti-inflammatory activity that involve inhibition of cycloxygease-2 and, phospholipase A 2 expression, and decrease levels of tumor necrosis factor-α, interleukin (IL)-1, IL-6, and nitric oxide.4) The anti-allergic activity is associated with marked inhibition of OVA-induced tracheal contraction and histamine release from lung tissue. The mast-cell degranulating peptide binds to the mast cell receptors and inhibits the binding of IgE and production of histamine.5) BV also inhibits the release of inflammatory mediators similar to nonsteroidal anti-inflammatory drugs.The anti-inflammatory effect of BV includes airborne ...

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Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-α production stimulated by LPS

Cited by 56 publications

References 39 publications

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro

Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line

Anti-allergic Effect of Bee Venom in an Allergic Rhinitis Mouse Model

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