Effect of Hot Water and Molybdenum Dips on Endogenous Polyamines and Heat Shock Protein (Hsp70) in Lemon Flavedo and Their Ability to Alleviate Chilling Injury During Cold Storage

Mathaba, N.; Tesfay, S.Z.; Bower, J.P.; Bertling, I.

doi:10.17660/actahortic.2013.1012.149

Cited by 2 publications

(2 citation statements)

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“…Many studies have shown that PA play important roles in the balance of reactive oxygen species (ROS) metabolism when plants respond to abiotic stress (Kim & Jin 2006; Andronis et al 2014). The PA have high‐density positive charges that can bind with negatively charged phosphate groups on the membrane, resulting in a reduction in cell membrane potential and the degree of lipid peroxidation (Mathaba et al 2013). Under salt stress, PA and Put can decrease excess ROS production in vivo by increasing antioxidant enzyme (SOD, CAT, APX) activities in foxtail millet ( Setaria italica L.) and Brassica juncea (Chinta et al 2015; Verma & Mishra 2005).…”

Section: Discussionmentioning

confidence: 99%

Metabolic regulation of polyamines and γ‐aminobutyric acid in relation to spermidine‐induced heat tolerance in white clover

Luo

Tang

et al. 2020

Plant Biol J

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• Heat stress decreases crop growth and yield worldwide. Spermidine (Spd) is a small aliphatic amine and acts as a ubiquitous regulator for plant growth, development and stress tolerance. • Objectives of this study were to determine effects of exogenous Spd on changes in endogenous polyamine (PA) and c-aminobutyric acid (GABA) metabolism, oxidative damage, senescence and heat shock protein (HSP) expression in white clover subjected to heat stress. Physiological and molecular methods, including colorimetric assay, high performance liquid chromatography and qRT-PCR, were applied. • Results showed that exogenous Spd significantly alleviated heat-induced stress damage. Application of Spd not only increased endogenous putrescine, Spd, spermine and total PA accumulation, but also accelerated PA oxidation and improved glutamic acid decarboxylase activity, leading to GABA accumulation in leaves under heat stress. The Spd-pretreated white clover maintained a significantly higher chlorophyll (Chl) content than untreated plants under heat stress, which could be related to the roles of Spd in up-regulating genes encoding Chl synthesis (PBGD and Mg-CHT) and maintaining reduced Chl degradation (PaO and CHLASE) during heat stress. In addition, Spd upregulated HSP70, HSP70B and HSP70-5 expression, which might function in stabilizing denatured proteins and helping proteins to folding correctly in white clover under high temperature stress. • In summary, exogenous Spd treatment improves the heat tolerance of white clover by altering endogenous PA and GABA content and metabolism, enhancing the antioxidant system and HSP expression and slowing leaf senescence related to an increase in Chl biosynthesis and a decrease in Chl degradation during heat stress.

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Section: Discussionmentioning

confidence: 99%

Metabolic regulation of polyamines and γ‐aminobutyric acid in relation to spermidine‐induced heat tolerance in white clover

Luo

Tang

et al. 2020

Plant Biol J

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“…Previous studies have noted that PAs play a critical role in balancing ROS metabolism under abiotic stress [58]. High-density, positively charged PAs can bind with (negatively charged) phosphate groups on the membrane, resulting in decreased membrane potential and lipid peroxidation levels [59]. PA and Put could substantially reduce ROS in foxtail millet (Setaria italica L.) and Brassica juncea [60] under salinity stress by increasing antioxidant activities [61].…”

Section: Discussionmentioning

confidence: 99%

Exogenous Putrescine Increases Heat Tolerance in Tomato Seedlings by Regulating Chlorophyll Metabolism and Enhancing Antioxidant Defense Efficiency

Jahan

Hasan

Alotaibi

et al. 2022

Plants

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Crops around the world are facing a diversity of environmental problems, of which high temperatures are proving to be the most serious threat to crops. Polyamine putrescine (Put) acts as a master growth regulator that contributes to optimal plant growth and development and increased stress tolerance. Here, the current study aimed to elucidate how Put functions in regulating chlorophyll (Chl) metabolism, oxidative stress, and antioxidant defense, as well as to characterize the expression of genes related to heat stress in tomato seedlings under such stress. The results revealed that Put treatment significantly attenuates heat-induced damage by promoting biomass production, increasing photosynthetic efficiency, and inhibiting excessive production of oxidative stress markers. Heat stress markedly decreased the Chl content in the tomato leaf and accelerated the leaf yellowing process. However, Put-treated tomato seedlings showed a higher Chl content, which could be associated with the functions of Put in elevating PBGD activity (Chl biosynthesis enzyme) and suppressing the activity of the Chl catabolic enzyme (Chlase and MDCase). Under high-temperature stress, the expression levels of the gene encoding factors involved in Chl biosynthesis and Chl catabolism were significantly down- and upregulated, respectively, and this trend was reversed in Put-treated heat-stressed seedlings. In addition, exogenous application of Put boosted the activity of antioxidant enzymes, along with the levels of expression of their encoding genes, only in plants that were heat stressed. Furthermore, the expression levels of heat-shock-related genes (HSP90, HSP70, and HsfA1) were elevated in Put-treated, high-temperature-stressed tomato seedlings. Taken together, our results indicate that Put treatment significantly increases the heat tolerance of tomato seedlings, by elevating Chl concentrations and suppressing Chl catabolic enzyme activity, modulating endogenous free PA content, increasing antioxidant defense efficiency, and upregulating the expression of heat-shock-related genes.

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Effect of Hot Water and Molybdenum Dips on Endogenous Polyamines and Heat Shock Protein (Hsp70) in Lemon Flavedo and Their Ability to Alleviate Chilling Injury During Cold Storage

Cited by 2 publications

References 0 publications

Metabolic regulation of polyamines and γ‐aminobutyric acid in relation to spermidine‐induced heat tolerance in white clover

Metabolic regulation of polyamines and γ‐aminobutyric acid in relation to spermidine‐induced heat tolerance in white clover

Exogenous Putrescine Increases Heat Tolerance in Tomato Seedlings by Regulating Chlorophyll Metabolism and Enhancing Antioxidant Defense Efficiency

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