Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/<i>β</i>‐Catenin Signaling Pathway in Diabetic Rats

Bai, Lijun; Huo, Bei‐Bei; Chen, Zhiqiang; Guo, Qian; Xu, Jing; Fang, Jing; Zhang, Jianghua; Zhang, Fenfang

doi:10.1155/2017/3054145

Cited by 7 publications

(9 citation statements)

References 30 publications

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“…Accumulating experiment and clinical studies have demonstrated that many traditional Chinese medicines (TCM) or active ingredients of TCM had preventive and therapeutic effects on kidney damage caused by diabetes [12]. Blood stasis blocking the collaterals of kidney is one of the main pathogeneses in DN and has been widely approved by the academic circles of TCM [13]. Our previous research also confirmed that Huayu Tongluo herbs had protective effect on renal tissues of diabetic rats [13].…”

Section: Introductionsupporting

confidence: 57%

“…After one week of adaptive rearing with regular rodent chow (protein 21.1%, fat 4.5%, carbohydrate 60.6%; Botai Hongda Biotechnology Co., Ltd., Beijing, China, http://biotech-hd.cn.gongchang.com), all rats were randomly assigned into the control group (C group, n � 8) and diabetic group (n � 40) according to the random number table method. e rats in the C group were still bred with regular rodent chow, while the diabetic group rats were bred with high-fat diet (HFD, protein 24.2%, fat 25.4%, carbohydrate 42.1%; Botai Hongda Biotechnology Co., Ltd., Beijing, China, http://biotech-hd.cn.gongchang.com) for the initial period of 6 weeks according to the method designed by us previously [13]. After fasting overnight, the diabetic group rats were administered intraperitoneal injection of low-dose streptozotocin (STZ, Enzo Life Science Co., Ltd., USA, 35 mg/ kg) diluted with 0.1 M cold citrate buffer (pH 4.3).…”

Section: Animal Modeling and Groupingmentioning

confidence: 99%

“…Blood stasis blocking the collaterals of kidney is one of the main pathogeneses in DN and has been widely approved by the academic circles of TCM [ 13 ]. Our previous research also confirmed that Huayu Tongluo herbs had protective effect on renal tissues of diabetic rats [ 13 ]. Hirudo, also known as Chinese medicinal leech, is a commonly used animal-derived TCM for promoting blood circulation and removing blood stasis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation

Yang

Guo

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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As diabetic nephropathy (DN) is one of the most common and destructive microvascular complications of diabetes mellitus, the goal of this study, therefore, was to investigate the renal protective effect and latent mechanisms of Hirudo lyophilized powder on diabetic rats. In this study, all rats were randomly assigned into the control group and diabetic group. The rats of diabetic group were injected with low-dose STZ (35 mg/kg) intraperitoneal plus high-fat diet to induce diabetes. Then, the successful diabetic model rats were weighed and randomly assigned into four groups: (1) diabetic model group (DM group); (2) Hirudo lyophilized powder 0.3 g/kg treatment group (SL group); (3) Hirudo lyophilized powder 0.6 g/kg treatment group (SM group); (4) Hirudo lyophilized powder 1.2 g/kg treatment group (SH group). Their fasting blood glucoses (FBG) were measured every 4 weeks. After treatment with Hirudo lyophilized powder at a corresponding dose once a day for 16 weeks, their metabolic and biochemical as well as oxidative stress parameters were tested, and the kidney weight (KW)/body weight (BW) was calculated. The renal tissues were used for histological, mRNA, and protein expression analysis. The results showed that Hirudo lyophilized powder could protect against the structural damages and functional changes of diabetic renal tissue by inhibiting oxidative stress, inflammation, and fibrosis. Furthermore, it was found in the further research that inhibiting the NOX4 expression and JAK2/STAT1/STAT3 pathway activation might be the underlying mechanisms. Collectively, Hirudo lyophilized powder might be a promising therapeutic agent for the treatment of DN.

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Section: Introductionsupporting

confidence: 57%

Section: Animal Modeling and Groupingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation

Yang

Guo

et al. 2021

Evidence-Based Complementary and Alternative Medicine

Self Cite

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“…For instance, An et al observed that hyperoside, a flavone glycoside with anti‐inflammatory, anti‐cancer and anti‐oxidan properties, prevents proteinuria in diabetic nephropathy . Furthermore, Bai et al found that Huayu Tongluo herbs inhibits the production of proteinuria in diabetic rats …”

Section: Discussionmentioning

confidence: 99%

Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β‐catenin activity

Huang

Xue

et al. 2019

J Cellular Molecular Medi

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Salidroside is a major phenylethanoid glycoside in Rhodiola rosea L., a traditional Chinese medicine, with multiple biological activities. It has been shown that salidroside possesses protective effects for alleviating diabetic renal dysfunction, contrast‐induced‐nephropathy and other kidney diseases. However, the involved molecular mechanism was still not understood well. Herein, we examined the protective effects of salidroside in mice with Adriamycin (ADR)‐induced nephropathy and the underlying molecular mechanism. The results showed that salidroside treatment ameliorates proteinuria; improves expressions of nephrin and podocin; and reduces kidney fibrosis and glomerulosclerosis induced by ADR. Mechanistically, ADR induces a robust accumulation of β‐catenin in the nucleus and stimulates its downstream target gene expression. The application of salidroside largely abolishes the nuclear translocation of β‐catenin and thus inhibits its activity. Furthermore, the activation of β‐catenin almost completely counteracts the protective roles of salidroside in ADR‐injured podocytes. Taken together, our data indicate that salidroside ameliorates proteinuria, renal fibrosis and podocyte injury in ADR nephropathy, which may rely on inhibition of β‐catenin signalling pathway.

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“…The Notch pathway is downstream of the Wnt/β-catenin signal and thus determines the destiny of HF. In addition, in cell culture, where HFSCs differentiate into hair and the Lef1 gene is upregulated, the Wnt transcriptional target genes activate the target gene Jagged1 and directly activate Wnt/β-catenin and Notch signaling pathways ( Bai et al, 2017 ). In contrast, in stratified IFE, β-catenin is detected in proliferating basal cells, and Notch1 is primarily expressed in the differentiated basal layer.…”

Section: The Wnt/β-catenin Signaling Pathway and The Notch Signaling mentioning

confidence: 99%

Regulatory Mechanisms of the Wnt/β-Catenin Pathway in Diabetic Cutaneous Ulcers

et al. 2018

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Skin ulcers are a serious complication of diabetes. Diabetic patients suffer from vascular lesions and complications such as peripheral neuritis, peripheral vascular lesions, and collagen abnormalities, which result in skin wounds that are refractory and often develop into chronic ulcers. The healing of skin ulcers requires an inflammatory reaction, wound proliferation, remodeling regulation, and control of stem cells. Studies investigating diabetic cutaneous ulcers have focused on cellular and molecular levels. Diabetes can cause nerve and blood vessel damage, and persistent high blood sugar levels can cause systemic multisite nerve damage based on peripheral neuropathy. The long-term hyperglycemia state enables the polyol glucose metabolism pathway to be activated, increasing the accumulation of toxic substances in the vascular injured nerve tissue cells. Sustained hyperglycemia leads to dysfunction of epithelial cells, leading to a decrease in pro-angiogenic signaling and nitric oxide production. In addition, due to impaired leukocyte function in hyperglycemia, immune function is impaired and the immune response at relevant sites is insufficient, making diabetic foot more difficult to heal. The Wnt/β-catenin pathway is a highly conserved signal transduction pathway involved in a variety of biological processes, such as cell proliferation, apoptosis, and differentiation. It is considered an important pathway involved in the healing of skin wounds. This article summarizes the mechanism of action of the Wnt/β-catenin pathway involved in the inflammatory responses to diabetic ulcers, wound proliferation, wound remodeling, and stem cells. The interactions between the Wnt signal pathway and other metabolic pathways are also discussed.

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Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/β‐Catenin Signaling Pathway in Diabetic Rats

Cited by 7 publications

References 30 publications

Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation

Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation

Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β‐catenin activity

Regulatory Mechanisms of the Wnt/β-Catenin Pathway in Diabetic Cutaneous Ulcers

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