Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours

Welsch, Clifford W.; Dombroske, S E; McManus, Michael J.; Calaf, Gloria M.

doi:10.1038/bjc.1979.278

Cited by 11 publications

(8 citation statements)

References 13 publications

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“…However, at higherconcentrations, above physiological, this preparation ofhGH was more effective than was the oPRL preparation. This observation is similar to reports of others (23,24). Clearly, however, both hGH and oPRL are lactogenic and have similar biological potency within the physiological range.…”

supporting

confidence: 92%

Membrane modification differentially affects the binding of the lactogenic hormones human growth hormone and ovine prolactin.

Bhattacharya¹,

Vonderhaar²

1981

Proc. Natl. Acad. Sci. U.S.A.

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Human growth hormone (hGH) and ovine prolactin (oPRL) are both lactogenic as defined by their ability to induce milk-protein synthesis in vitro in the presence of insulin and hydrocortisone. At physiological concentrations, both hGH and oPRL have similar dose-response curves in a mouse mammary gland organ culture system. Binding of "25I-labeled hGH ('2I-hGH) to lactogenic receptors is competed by both hGH and oPRL, and the competition curves are nearly superimposable. Moreover, solubilized membrane proteins bound with either '25I-hGH or '2MI-labeled oPRL show the same sedimentation pattern on sucrose gradients. However, methylation of membrane phospholipids in the presence of the methyl donor S-adenosyl-Lmethionine only increases the binding of "5I-hGH. Bindin of either '25I-oPRL or '25I-labeled bovine growth hormone (15I-bGH) was unaffected. Addition of concanavalin A (Con A) to the membranes decreased binding of "I5-oPRL to the lactogenic site by 80%, whereas 125I-hGH binding was decreased by only 40%, with the binding of '"I-bGH unaffected. However, both hGHand oPRL-bound proteins bind to Con A-Sepharose columns to the same extent. These results suggest that although hGH and oPRL bind to the same lactogenic site with similar affinities and elicit similar biological responses, modification ofmembranes either by phospholipid methylation or by Con A differentially affects the binding of these two hormones.

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supporting

confidence: 92%

Membrane modification differentially affects the binding of the lactogenic hormones human growth hormone and ovine prolactin.

Bhattacharya¹,

Vonderhaar²

1981

Proc. Natl. Acad. Sci. U.S.A.

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“…Several authors [25,26] have reported that both ovine and bovine prolactin stimulated DNA synthesis in the epithelium of such tissues. We had earlier found an increase in the 3H-thymidine incorporation into DNA in benign lesions in the presence of these hormones [16], but this increase was not significant. On the other hand, human prolactin had a significant effect on 2-day cultures of benign breast lesions.…”

Section: Discussionmentioning

confidence: 72%

“…We had previously studied the two most commonly analyzed experimental models for human breast cancer, i.e., the spontaneous mouse mammary carcinoma and the DMBA-induced rat mammary carcinoma [1,7], as well as benign [2,6,16,17] and malignant [1,4] lesions of human breast epithelium, and had found that insulin and hydrocortisone have the capacity to consistently stimulate DNA synthesis in tissue culture. Others (18) have reported a similar response with benign human breast tumors grown in athymic nude mice, suggesting the importance of such hormones in these systems.…”

Section: Discussionmentioning

confidence: 99%

Influence of hormones on DNA synthesis of breast tumors in culture

Calaf

Garrido

Moyano

et al. 1986

Breast Cancer Res Tr

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Tissue culture techniques have been developed for studying the influence of hormones on human breast tissues. The present study demonstrated that estrogen induced a highly significant increase in 3H-thymidine incorporation into DNA and labelling index of ductal epithelium of fibrocystic disease; there was no effect of progesterone, either alone or in combination with ovine prolactin, on benign lesions. Estrogen stimulated certain malignant tumors derived from postmenopausal women. These studies also showed that there was an inhibitory effect of 3H-thymidine incorporation into DNA by the effect of progesterone on malignant lesions. When menopausal status was considered, it was found that DNA synthesis was significantly higher in the presence of insulin and hydrocortisone in malignant tumors derived from premenopausal women than from postmenopausal women, or than in benign lesions. Thus, the present findings may provide evidence that specific activity may be an important measurement for breast tumor DNA synthesis in response to ovarian hormones or other substances.

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“…The prolactin receptor is expressed in almost all cell types and in 70% of breast cancer samples (Vonderhaar 1989). Early studies demonstrated that physiological concentrations of prolactin could induce proliferation of primary human breast tumors in organ culture and tumor-derived primary cells in culture (Welsch et al 1979;Malarkey et al 1983). In addition, prolactin induces proliferation of breast cancer-derived cell lines such as MCF-7 and T47D under serum-free conditions or in the presence of charcoal-stripped serum (Biswas and Vonderhaar 1987;Vonderhaar 1999).…”

Section: Prolactin and Breast Cancermentioning

confidence: 99%

Autocrine prolactin: an emerging market for homegrown (prolactin) despite the imports

Muthuswamy

2012

Genes Dev.

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Prolactin (PRL) is a peptide hormone that is produced by the pituitary gland and is known to regulate lactogenic differentiation. There is a significant body of evidence that points to autocrine production of prolactin and activation of an autocrine/paracrine signaling pathway to regulate cell proliferation and migration and inhibition of cell death. This perspective highlights the recent study in the October 1, 2012, issue of Genes & Development by Chen and colleagues (pp. 2154–2168) that describes a mechanism for autocrine prolactin production and places the finding in the context of a role for prolactin in breast development and cancer.

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Effect of human, bovine and ovine prolactin on DNA synthesis by organ cultures of benign human breast tumours

Cited by 11 publications

References 13 publications

Membrane modification differentially affects the binding of the lactogenic hormones human growth hormone and ovine prolactin.

Membrane modification differentially affects the binding of the lactogenic hormones human growth hormone and ovine prolactin.

Influence of hormones on DNA synthesis of breast tumors in culture

Autocrine prolactin: an emerging market for homegrown (prolactin) despite the imports

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