Effect of human endogenous retrovirus-K env gene knockout on proliferation of ovarian cancer cells

Ko, Eun‐Ji; Kim, Eun Taeg; Kim, Heungyeol; Lee, Chul Min; Koh, Suk Bong; Eo, Wan Kyu; Kim, Hongbae; Oh, Young Lim; Ock, Mee Sun; Kim, Ki Hyung; Cha, Hee‐Jae

doi:10.1007/s13258-022-01280-7

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…Previously, we reported that HERV-K env-related NUPR1 may activate the NUPR1 pathway in DLD-1 colorectal cancer cells through CRISPR-Cas9mediated KO of HERV-K env [18]. Furthermore, we knocked out HERV-K env to determine its function in ovarian cancer cells [19]. In this study, we performed HERV-K119 env KO in THP-1 and U937 human monocytic leukemia cells to analyze the role of HERV-K119 env in the immune response.…”

Section: Discussionmentioning

confidence: 99%

“…The role of HERVs in cancer has been reported in previous studies. We found that HERV-K Env protein expression was significantly increased in 11 types of cancers, and knockout (KO) of the HERV-K env gene using CRISPR-Cas9 can potentially reduce tumorigenic characteristics, including proliferation, invasion, and migration, in DLD-1 colorectal cancer cells and ovarian cancer cell lines [17][18][19]. Another study demonstrated that the downregulation of HERV-K env RNA in pancreatic cancer cells using shRNA resulted in reduced cell proliferation and tumor growth via the RAS-ERK-RSK pathway [20].…”

Section: Introductionmentioning

confidence: 99%

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The Role of Human Endogenous Retrovirus (HERV)-K119 env in THP-1 Monocytic Cell Differentiation

Ko,

Kim,

Kim

et al. 2023

IJMS

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Human endogenous retrovirus (HERV)-K was reportedly inserted into the human genome millions of years ago and is closely related to various diseases, including cancer and immune regulation. In our previous studies, CRISPR-Cas9-enabled knockout (KO) of the HERV-K env gene was found to potentially reduce cell proliferation, cell migration, and invasion in colorectal and ovarian cancer cell lines. The immune response involves the migration and invasion of cells and is similar to cancer; however, in certain ways, it is completely unlike cancer. Therefore, we induced HERV-K119 env gene KO in THP-1, a monocytic cell that can be differentiated into a macrophage, to investigate the role of HERV-K119 env in immune regulation. Cell migration and invasion were noted to be significantly increased in HERV-K119 env KO THP-1 cells than in MOCK, and these results were contrary to those of cancer cells. To identify the underlying mechanism of HERV-K119 env KO in THP-1 cells, transcriptome analysis and cytokine array analysis were conducted. Semaphorin7A (SEMA7A), which induces the production of cytokines in macrophages and monocytic cells and plays an important role in immune effector cell activation during an inflammatory immune response, was significantly increased in HERV-K119 env KO THP-1 cells. We also found that HERV-K119 env KO THP-1 cells expressed various macrophage-specific surface markers, suggesting that KO of HERV-K119 env triggers the differentiation of THP-1 cells from monocytic cells into macrophages. In addition, analysis of the expression of M1 and M2 macrophage markers showed that M1 macrophage marker cluster of differentiation 32 (CD32) was significantly increased in HERV-K119 env KO cells. These results suggest that HERV-K119 env is implicated in the differentiation of monocytic cells into M1 macrophages and plays important roles in the immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Role of Human Endogenous Retrovirus (HERV)-K119 env in THP-1 Monocytic Cell Differentiation

Ko,

Kim,

Kim

et al. 2023

IJMS

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“…The first scouting attempts on the real capabilities of electric powertrains in industrial vehicles were performed in the construction field as discussed in [108,109]. First excavators [110][111][112], then wheel loaders and bulldozers were electrified with different hybridization levels [113][114][115]. Nowadays, several manufacturers are already at a mature development stage and propose to the market electrified version of their top line products.…”

Section: Constructionmentioning

confidence: 99%

New Perspectives on Electric Vehicles

2022

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Institute of Electrical and Electronics Engineers (IEEE) and cofounder-member of the IEEE Power Electronics Romanian Chapter. He is a guest editor at Energies and an academic book editor for IntechOpen. He is also a member of the editorial boards of the Journal of Electrical Engineering, Electronics, Control and Computer Science and Sustainability. Dr. Gaiceanu has been General Chairman of the IEEE International Symposium on Electrical and Electronics Engineering in the last six editions. Contents Preface XIII Section 1 Electric Vehicles Infrastructures Chapter 1 Introductory Chapter: Towards 2050 NZE Pathway -Electric Transportation by Marian Găiceanu XIV VThe linear speed of the vehicle is controlled by an electronic differential controller that provides the reference speed for each drive wheel, which depends on the driver's reference speed and the steering angle.The last chapter, "Powerful Multilevel Simulation Tool for HiL Analysis of Urban Electric Vehicle's Propulsion Systems", presents the main assemblies of the traction system of an urban electric vehicle, its modelling being based on Macroscopic Energy Representation (EMR). The use of EMR facilitates the transition to hardware-in-theloop (HiL) implementation, replacing the simulated ensemble with the real one by using the communication between two professional software tools through National Instruments VeriStand software for real-time test applications. In this way, the analysis of advanced operation simulations of the propulsion unit of urban electric vehicles can be performed in real-time.The book is a useful resource for students, researchers, and professionals in electrical engineering.I wish to thank all the contributing authors as well as the staff at IntechOpen, particularly Author Service Manager Ms. Marina Dusevic, for all their support.

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“…Associations between the expression of speci c ERV families and cancer pathophysiology have been reported in various studies. For instance, the aberrant expression of human endogenous retrovirus family K (HERVK) sequences initiates cancer cell proliferation in solid tumors (19,20). It has also been reported that ERV expression initiates antineoplastic adaptive immune responses in cancers.…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of Human Endogenous Retrovirus K9-derived elements is associated with better clinical outcome of acute myelocytic leukemia

Yanagiya,

Nakagawa,

Onizuka

et al. 2024

Preprint

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Background: Acute myelocytic leukemia (AML) is a common hematological malignancy in adults. Although several risk stratifications based on cytogenetic and molecular abnormalities are available to decide the indication of allogeneic hematopoietic cell transplantation (allo-HCT), planning treatment strategies for AML without them remains challenging. Using transcriptome datasets, we investigated the association of event-free survival (EFS) of intensively treated AML cases and the aberrant expression status of endogenous retrovirus (ERV)-derived open reading frames (ORFs), which have been reported to be associated with the pathophysiology of various malignancies and have the potential to become neoantigens in specific cancers. Results: The expression values of human ERV family K9 (HERVK9) ORFs were found to be associated with EFS, independent of conventional risk stratifications. Furthermore, it was revealed that AML cells with higher expression of HERVK9 activated antigen processing and presentation, accompanied by excess expression of genes associated with responses to adaptive immune reaction and apoptosis, indicating that aberrant expression of HERVK9 may initiate an antineoplastic immune response against themselves via excess antigen presentation. Conclusions: In summary, quantitation of HERVK9 expression has the potential to provide prognostic prediction, which is crucial for determining the indications of upfront allo-HCT.

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Effect of human endogenous retrovirus-K env gene knockout on proliferation of ovarian cancer cells

Cited by 9 publications

References 15 publications

The Role of Human Endogenous Retrovirus (HERV)-K119 env in THP-1 Monocytic Cell Differentiation

The Role of Human Endogenous Retrovirus (HERV)-K119 env in THP-1 Monocytic Cell Differentiation

New Perspectives on Electric Vehicles

Aberrant expression of Human Endogenous Retrovirus K9-derived elements is associated with better clinical outcome of acute myelocytic leukemia

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