Effect of Human Erythrocyte Stromata on Complement Activation

Corrocher, Roberto; Tedesco, Francesco; Rabusin, P.; Sandre, G. De

doi:10.1111/j.1365-2141.1975.tb01817.x

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…It appears that the net effect of the C activators in the fluid of the LISR is to consume C and thus is inhibitory to the CF activity. This may explain the partial reduction of C4 AH activity by the excess of exogenous IgG, and may be an alternate reason for the finding (Corrocher et al, 1975) that lysis of PNH-like cells in the LISR is partially inhibitable by the RBC stroma.…”

Section: Discussionmentioning

confidence: 97%

The Low Ionic Strength Reaction of Human Blood: Relationship between the Binding of Serum Immunoglobulin and Complement to Red Blood Cells and Surface Charge of the Cells

et al. 1979

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Using the sucrose haemolysis reaction of Hartmann & Jenkins (1966) as a basic model, the low ionic strength reaction (LISR) of human blood was studied to determine: (1) serum Ig uptake by RBC with saline elution and 125I-IgG uptake, and (2) complement fixation (CF) to RBC with lysis of PNH cells and C3H/C4 antiglobulin haemagglutination (AH) of normal cells. The saline eluates were found to contain IgG and IgM with traces of IgA; their pH optima for the uptake by RBC were 6.0 +/- 0.5, 5.5 +/- 0.5 and c 5.0 respectively. The ratio of bound IgG to IgM was linearly related to the uptake pH. Both C4 AH and lysis were found to be optimum at pH 6.0--7.5, whereas the maximum C3 AH was at pH 6.0 +/- 0.5. The LISR performed at a constant pH (6.1 +/- 0.1) showed that an increasing concentration of neuraminidase (VCN) used in pretreatment of RBC was associated with a decrease in both IgG uptake and CF activity. A maximum VCN effect reduced the Ig uptake to c 20% of normal and abolished almost all the CF activity. An impaired LISR to various degrees was also observed with RBC pretreated with ficin, papain, bromelin, trypsin or protamine, and RBC from two individuals of En(a-) type. Preincubation of serum at LIS with and without RBC resulted in respectively a 'complete' and partial consumption of C in the fluid phase. The latter was not enhanced or inhibited by the addition of VCN-treated RBC for preincubation. A hypothesis is proposed suggesting that in the LSR the Ig uptake by RBC is an electrostatic interaction of the oppositely charged RBC and Ig and the CF to RBC results from C activation by the cell-bound IgG and IgM. In addition, a pH-dependent inactivation of the cell-bound C3 in the LISR is demonstrated.

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Section: Discussionmentioning

confidence: 97%

The Low Ionic Strength Reaction of Human Blood: Relationship between the Binding of Serum Immunoglobulin and Complement to Red Blood Cells and Surface Charge of the Cells

et al. 1979

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“…The Hedgehog (HH) signaling pathway is involved in embryonic development, repair of the normal tissues and EMT by controlling cell fate specification and pattern formation [81]. In mammals, there are three HH ligands proteins; Sonic hedgehog (SHH), Indian hedgehog (IHH), and Desert hedgehog (DHH).…”

Section: Signaling Pathways That Regulate Cancer Stem Cellsmentioning

confidence: 99%

Cancer Stem Cell and Gastrointestinal Cancer: Current Status, Targeted Therapy and Future Implications

Ahmad¹,

Dhawan²

2015

Biochem Pharmacol (Los Angel)

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The cancer stem cells (CSCs) are biologically distinct subset of rare cancer cells with inherent ability of self-renewal, de-differentiation, and capacity to initiate and maintain malignant tumor growth. Studies have further reported that CSCs prime cancer recurrence and therapy resistance. Therefore, targeting CSCs to inhibit cancer progression has become an attractive anti-cancer therapeutical strategy. Recent technical advances have provided a greater appreciation of the multistep nature of the oncogenesis and also clarified that CSC concept is not universally applicable. Irrespective, the role of the CSCs in gastrointestinal (GI) cancers, responsible for the most cancer-associated death, has been widely accepted and appreciated. However, despite the tremendous progress made in the last decade in developing markers to identify CSCs, and assays to assess tumorigenic function of CSCs, it remains an area of active investigation. In current article, we review findings related to the role and identification of CSCs in GI-cancers and discuss the crucial pathways involved in regulating CSCs populations’ development and drug resistance, and use of the tumoroid culture to test novel CSCs-targeted cancer therapies.

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“…Indeed, they share the main laboratory features of PNH cells: low membrane AChE [5] and lysis when exposed to complement whatever the me chanism of its activation [2,8,9,12,14], The low AChE activity might suggest that also the uptake of choline by these cells should be diminished, at least in the pres ence of acetylcholine, since the hydrolysis of the molecule is essential for the uptake of choline moiety (neither whole acetyl choline nor acetate can pass the RBC membrane [7]). We have already demon strated that this is not the case: the uptake of choline by the PNH RBC incubated with acetylcholine is higher than normal, as it is when the cells are incubated with choline [1].…”

Section: Discussionmentioning

confidence: 99%

Effects on Red Blood Cell Choline Transport Induced by Different Sulfhydryl Compounds

Falezza¹,

Olivieri²,

Corrocher³

et al. 1984

Acta Haematol

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The uptake of choline was studied in different types of paroxysmal nocturnal hemoglobinuria (PNH)-like RBC obtained from normal human erythrocytes by the action of different sulfhydryl compounds. The results were compared with the uptake of choline in true PNH cells and in normal reticulocytes. The PNH-like cells which better mimicked true PNH were those prepared using acetylcysteine. Indeed these erythrocytes displayed a capacity to incorporate choline against a concentration gradient exactly as PNH cells exposed to either acetylcholine or choline. The uptake of choline by the other PNH-like cells differed from that of PNH erythrocytes in several ways. The results suggest that every sulfhydryl compound induces a different damage on RBC membrane.

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Effect of Human Erythrocyte Stromata on Complement Activation

Cited by 16 publications

References 16 publications

The Low Ionic Strength Reaction of Human Blood: Relationship between the Binding of Serum Immunoglobulin and Complement to Red Blood Cells and Surface Charge of the Cells

The Low Ionic Strength Reaction of Human Blood: Relationship between the Binding of Serum Immunoglobulin and Complement to Red Blood Cells and Surface Charge of the Cells

Cancer Stem Cell and Gastrointestinal Cancer: Current Status, Targeted Therapy and Future Implications

Effects on Red Blood Cell Choline Transport Induced by Different Sulfhydryl Compounds

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