Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity

Li, Shengnan; Guan, Xiuchen; Yu, Wenting; Zhao, Zeqing; Sun, Yaxi; Bai, Yuxing

doi:10.1111/odi.14671

Cited by 4 publications

(2 citation statements)

References 78 publications

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“…A recent study investigated the development of an in-situ tissue engineering scaffold (iTE-scaffold) using the coaxial electrospinning technique to sequentially deliver bFGF and BMP-2 [65][66][67][68]. The iTE-scaffold was found to effectively enhance the angiogenesis of periodontal ligament stem cells (PDLSCs) and induce macrophage polarization into the prohealing M2 phenotype, thereby modulating inflammation [7].…”

Section: Novel Findings In Scaffolds Incorporating Growth Factors For...mentioning

confidence: 99%

“…Regenerative medicine encompasses the substitution or rejuvenation of human cells, tissues, or organs. Langer and Vacanti propose that tissue engineering comprises a triad, which includes stem/progenitor cells, scaffolds supporting cell growth, and essential growth factors [65][66][67]. Termed the paracrine effect, stem cells demonstrate an exceptional ability to release cytokines locally, thus promoting regenerative processes and influencing the balance between tissue regeneration and degeneration.…”

Section: Cell-based Tissue Engineeringmentioning

confidence: 99%

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Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Angjelova,

Jovanova,

Polizzi

et al. 2024

Medicina

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The regeneration of periodontal bone defects continues to be an essential therapeutic concern in dental biomaterials. Numerous biomaterials have been utilized in this sector so far. However, the immune response and vascularity in defect regions may be disregarded when evaluating the effectiveness of biomaterials for bone repair. Among several regenerative treatments, the most recent technique of in situ tissue engineering stands out for its ability to replicate endogenous restorative processes by combining scaffold with particular growth factors. Regenerative medicine solutions that combine biomaterials/scaffolds, cells, and bioactive substances have attracted significant interest, particularly for bone repair and regeneration. Dental stem cells (DSCs) share the same progenitor and immunomodulatory properties as other types of MSCs, and because they are easily isolable, they are regarded as desirable therapeutic agents in regenerative dentistry. Recent research has demonstrated that DSCs sown on newly designed synthetic bio-material scaffolds preserve their proliferative capacity while exhibiting increased differentiation and immuno-suppressive capabilities. As researchers discovered how short peptide sequences modify the adhesion and proliferative capacities of scaffolds by activating or inhibiting conventional osteogenic pathways, the scaffolds became more effective at priming MSCs. In this review, the many components of tissue engineering applied to bone engineering will be examined, and the impact of biomaterials on periodontal regeneration and bone cellular biology/molecular genetics will be addressed and updated.

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Section: Novel Findings In Scaffolds Incorporating Growth Factors For...mentioning

confidence: 99%

Section: Cell-based Tissue Engineeringmentioning

confidence: 99%

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Angjelova,

Jovanova,

Polizzi

et al. 2024

Medicina

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Extracellular Vesicles from Compression‐Loaded Cementoblasts Promote the Tissue Repair Function of Macrophages

Yang,

Liu,

Guo

et al. 2024

Advanced Science

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Treatment strategies for hard tissue defects aim to establish a mineralized microenvironment that facilitates tissue remodeling. As a mineralized tissue, cementum shares a similar structure with bone and exhibits an excellent capacity to resist resorption under compression. Macrophages are crucial for mineralized remodeling; however, their functional alterations in the microenvironment of cementum remain poorly understood. Therefore, this study explores the mechanisms by which cementum resists resorption under compression and the regulatory roles of cementoblasts in macrophage functions. As a result, extracellular vesicles from compression‐loaded cementoblasts (Comp‐EVs) promote macrophage M2 polarization and enhance the clearance of apoptotic cells (efferocytosis) by 2‐ to 3‐fold. Local injection of Comp‐EVs relieves cementum destruction in mouse root resorption model by activating the tissue repair function of macrophages. Moreover, Comp‐EV‐loaded hydrogels achieve significant bone healing in calvarial bone defect. Unexpectedly, under compression, EV secretion in cementoblasts is reduced by half. RNA‐Seq analysis and verification reveal that Rab35 expression decreases by 60% under compression, thereby hampering the release of EVs. Rab35 overexpression is proposed as a modification of cementoblasts to boost the yield of Comp‐EVs. Collectively, Comp‐EVs activate the repair function of macrophages, which will be a potential therapeutic strategy for hard tissue repair and regeneration.

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Critical roles of extracellular vesicles in periodontal disease and regeneration

Jing,

Wang,

Zhang

et al. 2024

Stem Cells Translational Medicine

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Extracellular vesicles (EVs) are evolutionarily conserved communication mediators that play key roles in the development of periodontal disease as well as in regeneration processes. This concise review first outlines the pathogenic mechanisms through which EVs derived from bacteria lead to the progression of periodontitis, with a focus on the enrichment of virulence factors, the amplification of immune responses, and the induction of bone destruction as key aspects influenced by bacterial EVs. This review aims to elucidate the positive effects of EVs derived from mesenchymal stem cells (MSC-EVs) on periodontal tissue regeneration. In particular, the anti-inflammatory properties of MSC-EVs and their impact on the intricate interplay between MSCs and various immune cells, including macrophages, dendritic cells, and T cells, are described. Moreover, recent advancements regarding the repair-promoting functions of MSC-EVs are detailed, highlighting the mechanisms underlying their ability to promote osteogenesis, cementogenesis, angiogenesis, and the homing of stem cells, thus contributing significantly to periodontal tissue regeneration. Furthermore, this review provides insights into the therapeutic efficacy of MSC-EVs in treating periodontitis within a clinical context. By summarizing the current knowledge, this review aims to provide a comprehensive understanding of how MSC-EVs can be harnessed for the treatment of periodontal diseases. Finally, a discussion is presented on the challenges that lie ahead and the potential practical implications for translating EV-based therapies into clinical practices for the treatment of periodontitis.

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Effect of human periodontal ligament stem cell‐derived exosomes on cementoblast activity

Cited by 4 publications

References 78 publications

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Extracellular Vesicles from Compression‐Loaded Cementoblasts Promote the Tissue Repair Function of Macrophages

Critical roles of extracellular vesicles in periodontal disease and regeneration

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