Effect of human seminal fluid on the growth of endometrial cells of women with endometriosis

Khan, Khaleque Newaz; Kitajima, Michio; Hiraki, Koichi; Fujishita, Akira; Sekine, Ichiro; Ishimaru, Tadayuki; Masuzaki, Hiroaki

doi:10.1016/j.ejogrb.2009.12.022

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…Both in vitro and in vivo studies have shown that female genital tract cervicovaginal cells exposed to seminal plasma have increased expression of GM-CSF, IL-6, IL-8, MCP-1, MIP-3α, IL-1α, and IL-1β (4, 6, 10, 21). This study was consistent with these previous findings, confirming the hypothesis that semen up-regulates pro-inflammatory gene expression in cells of the female genital tract.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells

et al. 2018

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Genital inflammatory cytokine responses increase HIV risk. Since male partner semen is a complex mixture of immune-modulatory prostaglandins and cytokines, we hypothesized that exposure to semen may influence genital inflammation in women. Here, we investigated cytokine response kinetics of cervical cells following stimulation with seminal plasma from HIV-negative and HIV-positive men characterized as having low or high concentrations of inflammatory cytokines. Irrespective of the HIV status or semen cytokine profile, in vitro stimulation of cervical cells with seminal plasma resulted in significantly elevated concentrations of secreted IL-6, IL-8, TNF-β, MCP-1, GM-CSF, and VEGF within 8 h of stimulation, which tended to decline by 24 h, although this was only significant for TNF-β. Consistent with this, cervical cells responded to seminal plasma with increases in IL-8 and IL-1β mRNA expression of 10-fold. These findings suggest that the impact of semen on local female genital cytokines is likely transient. Although these findings suggest that the impact of semen on local female genital cytokines may not be sustained long-term, this heightened genital inflammation may have implications for HIV risk in women.

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Section: Discussionmentioning

confidence: 99%

Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells

et al. 2018

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“…It may be that the fertility-promoting effects of seminal fluid are balanced against a health and evolutionary cost. Seminal plasma promotes the growth of endometrial gland and stromal cells derived from women with endometriosis in vitro (38), and endometrial fragments from infertile women respond similarly with more aggressive lesion development in a mouse model of endometriosis (31), raising the question of whether semen-derived factors influence aberrant tissue persistence in endometriosis. An issue of current interest to the HIV field is whether the immune-regulatory effects of seminal plasma, particularly the high content of TGFB and prostaglandins, may advantage transmission of and suppress immune defense against HIV and other STIs, which potentially have evolved to take advantage of the toleranceinducing functions of seminal plasma (39,40).…”

Section: Effects Of Seminal Fluid On the Immune Response In Womenmentioning

confidence: 99%

Seminal fluid and fertility in women

Robertson

Sharkey

2016

Fertility and Sterility

180

126

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“…16 In vitro experiments have demonstrated increased proliferation of human endometrial epithelial cells after the addition of seminal plasma to culture media. 17,18 This finding suggests the possibility that in women, seminal plasma exposure might enhance epithelial cell proliferation in retrograde menstrual endometrium, contributing to the development or progression of endometriosis.…”

mentioning

confidence: 89%

Seminal Plasma Promotes Lesion Development in a Xenograft Model of Endometriosis

McGuane

Watson

Zhang

et al. 2015

The American Journal of Pathology

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The factors that predispose one-tenth of reproductive-aged women to endometriosis are poorly understood. We determined that genetic deficiency in transforming growth factor β1 impairs endometriosis-like lesion growth in mice. Given that seminal plasma is an abundant source of transforming growth factor β, we evaluated the effect of exposure to seminal plasma on the growth of endometrial lesions. Human endometrial explants were exposed to seminal plasma or to control medium before transfer to Prkdc(scid)-mutant (severe combined immunodeficient) mice. Xenografts exposed to seminal plasma showed an eightfold increase in volume and a 4.3-fold increase in weight after 14 days. These increases were associated with increased proliferation of endometrial epithelial cells and enhanced survival and proliferation of human stromal cells compared with those in control lesions, in which human stromal cell persistence was negligible. Although the distribution of macrophages was altered, their number and activation status did not change in response to seminal plasma. Seminal plasma stimulated the production of a variety of cytokines in endometrial tissue, including growth-regulated oncogene, granulocyte macrophage colony-stimulating factor, and IL-1β. These data suggest that seminal plasma enhances the formation of endometriosis-like lesion via a direct effect on endometrial cell survival and proliferation, rather than via macrophage-mediated mechanisms. These findings raise the possibility that endometrial exposure to seminal plasma could contribute to endometriotic disease progression in women.

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Effect of human seminal fluid on the growth of endometrial cells of women with endometriosis

Cited by 11 publications

References 30 publications

Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells

Inflammatory Cytokine Profiles of Semen Influence Cytokine Responses of Cervicovaginal Epithelial Cells

Seminal fluid and fertility in women

Seminal Plasma Promotes Lesion Development in a Xenograft Model of Endometriosis

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