Effect of Human Umbilical Cord Blood Progenitor Cells Versus Mononuclear Cells on Acute Renal Failure Rat Model

Mohamed, Magda I.; Attia, Fadia M.; Atwa, Khaled A.

doi:10.2174/157488811797904344

Cited by 5 publications

(7 citation statements)

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“…In the research of nephrology, hUCBMNCs can reduce SCr and BUN in the rats with diabetic nephropathy [10]. hUCBMNCs also can improve the renal function of nephrotoxic kidney [35]. In the studies above, there was no standard for the dosage of hUCBMNCs.…”

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confidence: 99%

Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Feng

Zhao

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Acute kidney injury (AKI) is one of the common complications of sepsis. Heretofore, there is no effective treatment for septic AKI. Recent studies have revealed that besides treating hematological malignancies, human umbilical cord blood mononuclear cells (hUCBMNCs) show good therapeutic effects on other diseases. But whether hUCBMNCs can protect against septic AKI and its underlying mechanism are unknown. Methods. The rat model of lipopolysaccharide- (LPS-) induced AKI was developed, and the injection of hUCBMNCs was executed to prevent and treat AKI. ML385, a specific nuclear factor E2-related factor 2 (Nrf2) inhibitor, was used to silence Nrf2. The cell experiments were conducted to elaborate the protective mechanism of Nrf2 pathway. Results. An effective model of LPS-induced AKI was established. Compared to the rats only with LPS injection, the levels of inflammation, reactive oxygen species (ROS), and apoptosis in renal tissues after hUCBMNC injection were markedly attenuated. Pathological examination also indicated significant remission of renal tissue injury in the LPS+MNCs group, compared to rats in the LPS group. Transmission electron microscopy (TEM) showed that the damage of the mitochondria in the LPS+MNCs group was lighter than that in the LPS group. Noteworthily, the renal Nrf2/HO-1 pathway was activated and autophagy was enhanced after hUCBMNC injection. ML385 could partly reverse the renoprotective effect of hUCBMNCs, which could demonstrate that Nrf2 participated in the protection of hUCBMNCs. Cell experiments showed that increasing the expression level of Nrf2 could alleviate LPS-induced cell injury by increasing the autophagy level and decreasing the injury of the mitochondria in HK-2 cells. Conclusion. All results suggest that hUCBMNCs can protect against LPS-induced AKI via the Nrf2 pathway. Activating Nrf2 can upregulate autophagy to protect LPS-induced cell injury.

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confidence: 99%

Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Feng

Zhao

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…HUCB has emerged as a source of cells with therapeutic potential in many medical fields ( 34 ). Previous studies have shown that HUCB MNCs are useful for treating hematopoietic and genetic disorders ( 23 ), diabetes ( 30 ), AKI caused by glycerol ( 26 ) and diabetic nephropathy ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

“…After injection with GM, the use of HUCB MNCs showed a reduction in chromosomal abnormalities and gene biomarkers (Kim-1, NGAL) in the treated group. KIM-1 is usually absent but activated when the proximal tubular apical membrane is damaged due to tubular necrosis with a consequent decrease in the number of functioning nephrons ( 26 ). Hence, we suggested that HUCB-MNCs could restore the normal expression of Kim-1 level by minimizing the observed renal damage.…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that mesenchymal stem cells (MSCs) may help treat AKI ( 25 ). Furthermore, stem cell transplantation has been proposed to treat glycerol-induced renal toxicity, which is associated with critical histopathological changes in renal tissue and enhanced kidney function tests ( 26 ). The kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) biomarkers were used to classify renal injury earlier and more specifically due to the low sensitivity and specificity of serum creatinine (SCr) and blood urea nitrogen (BUN) ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

“…The kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) biomarkers were used to classify renal injury earlier and more specifically due to the low sensitivity and specificity of serum creatinine (SCr) and blood urea nitrogen (BUN) ( 27 ). Kidney injury molecule-1 (Kim-1 in rodents, KIM-1 in humans) ( 28 ) is a type 1 transmembrane protein, usually absent but activated when the proximal tubular apical membrane is damaged ( 26 , 27 ). NGAL is a protein that was first identified in human neutrophils and has since been found in immune cells, various tissues, and organs such as the trachea, lung, intestine, liver, colon, and kidney ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

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Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats

et al. 2021

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Background: Acute kidney injury (AKI), also known as acute renal failure (ARF), has received considerable critical attention in recent years. Gentamicin (GM) is an antibiotic whose prolonged use results in AKI with a high mortality rate.Methods: Fifty adult female albino rats weighing 150–200 g were used. The animals were divided into five groups: the first group was the normal healthy control one, the second group received only 1 × 106 HUCB mononuclear cells (MNCs)/rat by intravenous (iv) injection, the third diseased group was given GM 100 mg/kg for 10 consecutive days by intraperitoneal injections, the fourth preventive group received 1 × 106 HUCB MNCs/rat by iv injection 24 h before gentamicin treatment, and the fifth treated group received 1 × 106 HUCB MNCs/rat by iv injection 24 h after gentamicin treatment. After 1 week of treatment, blood samples were collected, and kidneys were removed for histopathological examination.Results: Rats treated with HUCB MNCs in the treated group had a significant decrease in renal damage, low levels of biomarkers for nephrotoxicities such as serum creatinine and blood urea nitrogen, and low chromosomal aberrations compared to the diseased third group. The gene expression of KIM-1 and NGAL was decreased in response to HUCB treatment.Conclusions: HUCB MNCs have a curative effect against AKI and gentamicin-induced genotoxicity owing to their regenerative property.

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Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats

Li¹,

Feng²,

Zhu³

et al. 2020

Biomedicine & Pharmacotherapy

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Effect of Human Umbilical Cord Blood Progenitor Cells Versus Mononuclear Cells on Acute Renal Failure Rat Model

Cited by 5 publications

References 0 publications

Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats

Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats

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