Effect of hyaluronan-enriched transfer medium on implantation and pregnancy rates after day 3 and day 5 embryo transfers: a prospective randomized study

Urman, Bülent; Yakın, Kayhan; Ata, Barış; Isiklar, Aycan; Balaban, Başak

doi:10.1016/j.fertnstert.2007.07.1294

Cited by 73 publications

(60 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hyaluronan can substitute for albumin in culture media but what is perhaps more significant for human IVF applications is that the addition of hyaluronan to a culture medium containing albumin significantly increased development but also improved the cryosurvival of embryos [22,23]. In addition, recent randomized controlled trials in clinical IVF confirmed that elevating the levels of hyaluronan increased pregnancy and implantation rates, which was particularly evident in selected patient groups [24][25][26]. Second is the different form of glutamine within G III series (alanyl-glutamine) compared with ISM culture media (L-glutamine).…”

Section: Discussionmentioning

confidence: 99%

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

Sifer

Handelsman

Grange

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To compare the effects of 2 different media on embryo morphology and development at days 2/3. Method Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n=286) or ICSI (n=390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates. Conclusions Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.

show abstract

Section: Discussionmentioning

confidence: 99%

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

Sifer

Handelsman

Grange

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…However, these methods are liable to fail among patients with multiple embryo transfer failures, on the basis that impaired interaction between the embryo and endometrium might be an underlying problem. Urman and coworkers demonstrated that the use of HA-enriched transfer medium (HETM) increased implantation and clinical pregnancy rates, both in general and among women with previous implantation failures [5].…”

Section: Introductionmentioning

confidence: 99%

“…The implantation rate was shown to be increased in women receiving either cleavage-stage embryos or blastocysts [5]. However, the effectiveness of HETM is controversial.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Nakagawa¹,

Takahashi²,

Nishi³

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To ascertain whether the use of hyaluronanenriched transfer medium (HETM) improves pregnancy and implantation rates among embryo transfer patients with a history of multiple implantation failures. Methods Patients (n0314) under the age of 40 and with a history of multiple unsuccessful embryo transfers were enrolled. There were three groups of patients: those undergoing fresh embryo transfer (fresh ET [n 0111]), those undergoing vitrified-warmed ET in the natural cycle (WET-N [n0101]) and those undergoing WET in a hormone replacement cycle ). On the day of ET, patients were randomized to HETM (0.5 mg/ml hyaluronan) or control medium containing no hyaluronan. Only patients with good quality embryos on day 3 were included. Results For all three patients groups (fresh ET, WET-N and WET-H) pregnancy rates (37.5 %, 31.4 % and 41.2 %, respectively) were significantly higher when using HETM compared with control medium (10.9 %, 10.0 % and 15.7 %, respectively; p<0.05), and implantation rates when using HETM were also significantly higher compared with control medium (p<0.05). Miscarriage rates were similar in both groups. Conclusion HETM significantly increased pregnancy and implantation rates among embryo transfer patients with a history of multiple unsuccessful implantations-regardless of method used to prepare the endometrium.

show abstract

“…In the human uterus, peak expression of HAS and CD44 is in the mid-secretory stage (Afify et al 2006). There is a plethora of data suggesting the beneficial roles for HA in human embryo implantation (Urman et al 2008, Hambiliki et al 2010, Nakagawa et al 2012. It is thought that implantation failure could be reduced by providing a 'sticky' matrix for the embryos to attach and for this reason HA (which is also called 'magic glue' (Girish & Kemparaju 2007), or EmbryoGlue (Hazlett et al 2008)) is often used as a supplement in human embryo transfer medium.…”

Section: Embryo Implantation-contrasting Datamentioning

confidence: 99%

Regulation and roles of the hyaluronan system in mammalian reproduction

et al. 2017

View full text Add to dashboard Cite

Hyaluronan (HA) is a non-sulphated glycosaminoglycan polymer naturally occurring in many tissues and fluids of mammals, including the reproductive system. Its biosynthesis by HA synthase (HAS1-3) and catabolism by hyaluronidases (HYALs) are affected by ovarian steroid hormones. Depending upon its molecular size, HA functions both as a structural component of tissues in the form of high-molecular-weight HA or as a signalling molecule in the form of small HA molecules or HA fragments with effects mediated through interaction with its specific cell-membrane receptors. HA is produced by oocytes and embryos and in various segments of the reproductive system. This review provides information about the expression and function of members of the HA system, including HAS, HYALs and HA receptors. We examine their role in various processes from folliculogenesis through oocyte maturation, fertilisation and early embryo development, to pregnancy and cervical dilation, as well as its application in assisted reproduction technologies. Particular emphasis has been placed upon the role of the HA system in pre-implantation embryo development and embryo implantation, for which we propose a hypothetical sequential model. Reproduction (2017) 153 R43-R58

show abstract

Effect of hyaluronan-enriched transfer medium on implantation and pregnancy rates after day 3 and day 5 embryo transfers: a prospective randomized study

Cited by 73 publications

References 37 publications

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures

Regulation and roles of the hyaluronan system in mammalian reproduction

Contact Info

Product

Resources

About