Effect of hydro- and osmopriming of chickpea (Cicer arietinum L.) seeds on enzymes of sucrose and nitrogen metabolism in nodules

Abstract

Three year data on the effect of waterand mannitol (4%) priming of chickpea seeds (12 h at 25°C) showed higher number and biomass of nodules in the plants from primed seeds than from non-primed seeds. The biomass of nodules increased to 75 DAS but decreased by 90 DAS. Activities of sucrose metabolism enzymes (sucrose synthase (SS) and alkaline invertase) and of nitrogen metabolism (glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH)) in nodules of primed and non-primed crops during development are reported. SS and alkaline invertase activities increased to 70 DAS and then decreased. In primed plants, the higher SS activity in nodules at 60 and 70 DAS might be responsible for providing more energy and carbon skeleton for nitrogen fixation and for ammonium assimilation in primed plants. At 85 DAS, though the SS activity decreased in comparison with the earlier growth stages, it was still higher in nodules of the primed crops than the non-primed crop. Activity of alkaline invertase was maximum at 70 DAS in the nodules of primed and non-primed crops. Priming increased nodule GS activity at 70 and 85 DAS. GOGAT activity was unaffected by priming but GDH activity was greater in nodules from primed crops at 50 DAS. Elevated SS and GS nodule activities in primed chickpeas might be responsible in increasing nodule biomass and metabolic activity thereby increasing seed fill.

Low sodium chloride priming increases seedling vigor and stress tolerance to Ralstonia solanacearum in tomato

“…However, in the case of seed priming, the e ect continues longer (Bruce et al 2007). It has been reported that elevated sucrose synthase and glutamate synthase activities were observed in the seed-primed nodule of the chickpea, and these elevated activities might be responsible for increasing the nodule biomass and metabolic activity, thereby increasing seed ll (Kaur et al 2006). us, the acquired tolerance to abiotic and biotic stress observed in the present study suggests that the e ect of the seed-priming treatment continues longer and is more e ective than the plant-priming treatment.…”

Optimal conditions for hydropriming lucerne seeds

“…Seed priming strategy has been widely used to resolve the storage problem by improving the seed vigour, and is successful in many crops, ornamental plants, and forages (Heydecker et al 1973;Rush 1991;Finch-Savage et al 2004;Kaur et al 2006). in priming, seeds are exposed to restricted water availability under controlled conditions which allows some of the physiological processes of germination to occur and then, before germination is completed, the seeds are usually redried for short-term storage before sowing (Wright et al 2003).…”

Alfalfa and Its Symbiosis Responses to Osmotic Stress

“…The irst technique consists in exposing the seeds to a limited quantity of water in a continuous or successive manner at a suitable temperature. This is an inexpensive technique often used for ield-grown cereals and legumes to accelerate germination [81]. The second technique consists in treating the seeds with microbiological agents such as rhizobia, Azospirillum, Pseudomonas, Bacillus, Trichoderma, Gliocladium and other species and aims to improve the vigour and viability of the seeds.…”

“…It has been shown that it can improve nodulation, N 2 capacity in legumes and nutrient acquisition, especially in less fertile soils [81]. Studies have reported that plants from treated seeds have shown high capacity to form nodules and accumulate large amounts of N, K + and P, especially under stress conditions [93,94].…”