Effect of hydrodynamics on the formation and removal of microalgal biofilm in photobioreactors

Bělohlav, Vojtěch; Zakova, Terezia; Jirout, Tomáš; Krátký, Lukáš

doi:10.1016/j.biosystemseng.2020.10.014

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…The agreement between τ crit-exp = 0.3 Pa based on measurements and the τ crit-theo = 0.4 Pa based on theoretical calculation (Equation 3) confirms our hypothesis that the critical conditions to develop biofilms is controlled by local flow velocity. Our measured τ crit-flat = 0.3 Pa is also consistent with some previous studies using other microorganisms (e.g., Belohlav et al, 2020;Roosjen et al, 2005).…”

Section: Impacts Of Surface Roughness On the Critical Condition To De...supporting

confidence: 92%

“…For example, the critical shear stress for microalgae Chlorella vulgaris biofilms to develop on the flat surface of photobioreactor panel is 0.2 Pa (Belohlav et al, 2020) and the critical shear stress to prevent adhesion for the yeast strains on smooth glass is 0.36 Pa (Roosjen et al, 2005). Note that here we assume that early-stage biofilms are highly porous and do not affect the flow field.…”

Section: Impacts Of Surface Roughness On the Critical Condition To De...mentioning

confidence: 99%

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Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms

Wei

Yang

2023

Biotech & Bioengineering

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Biofilms can increase pathogenic contamination of drinking water, cause biofilm‐related diseases, alter the sediment erosion rate, and degrade contaminants in wastewater. Compared with mature biofilms, biofilms in the early‐stage have been shown to be more susceptible to antimicrobials and easier to remove. Mechanistic understanding of physical factors controlling early‐stage biofilm growth is critical to predict and control biofilm development, yet such understanding is currently incomplete. Here, we reveal the impacts of hydrodynamic conditions and microscale surface roughness on the development of early‐stage Pseudomonas putida biofilm through a combination of microfluidic experiments, numerical simulations, and fluid mechanics theories. We demonstrate that early‐stage biofilm growth is suppressed under high flow conditions and that the local velocity for early‐stage P. putida biofilms (growth time < 14 h) to develop is about 50 μm/s, which is similar to P. putida's swimming speed. We further illustrate that microscale surface roughness promotes the growth of early‐stage biofilms by increasing the area of the low‐flow region. Furthermore, we show that the critical average shear stress, above which early‐stage biofilms cease to form, is 0.9 Pa for rough surfaces, three times as large as the value for flat or smooth surfaces (0.3 Pa). The important control of flow conditions and microscale surface roughness on early‐stage biofilm development, characterized in this study, will facilitate future predictions and managements of early‐stage P. putida biofilm development on the surfaces of drinking water pipelines, bioreactors, and sediments in aquatic environments.

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Section: Impacts Of Surface Roughness On the Critical Condition To De...supporting

confidence: 92%

Section: Impacts Of Surface Roughness On the Critical Condition To De...mentioning

confidence: 99%

Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms

Wei

Yang

2023

Biotech & Bioengineering

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“…A pilot-scale flat-panel photobioreactor and laboratory tubular system Completely removal with the wall shear stress of 53 Pa. Belohlav et al, 2020 Surface waves…”

Section: Chlorella Vulgaris (Microalgae) Hydrodynamicsmentioning

confidence: 99%

Plasma‐activated liquids for mitigating biofilms on food and food contact surfaces

Zhao

Bhavya

Patange

et al. 2023

Comp Rev Food Sci Food Safe

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Plasma‐activated liquids (PALs) are emerging and promising alternatives to traditional decontamination technologies and have evolved as a new technology for applications in food, agriculture, and medicine. Contamination caused by foodborne pathogens and their biofilms has posed challenges and concerns to the food industry in terms of safety and quality. The nature of the food and the food processing environment are major factors that contribute to the growth of various microorganisms, followed by the biofilm characteristics that ensure their survival in severe environmental conditions and against traditional chemical disinfectants. PALs show an efficient impact against microorganisms and their biofilms, with various reactive species (short‐ and long‐lived ones), physiochemical properties, and plasma processing factors playing a crucial role in mitigating biofilms. Moreover, there is potential to improve and optimize disinfection strategies using a combination of PALs with other technologies for the inactivation of biofilms. The overarching aim of this study is to build a better understanding of the parameters that govern the liquid chemistry generated in a liquid exposed to plasma and how these translate into biological effects on biofilms. This review provides a current understanding of PALs‐mediated mechanisms of action on biofilms; however, the precise inactivation mechanism is still not clear and is an important part of the research. Implementation of PALs in the food industry could help overcome the disinfection hurdles and can enhance biofilm inactivation efficacy. Future perspectives in this field to expand existing state of the art to seek breakthroughs for scale‐up and implementation of PALs technology in the food industry are also discussed.

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“…De fato, alguns microrganismos afetam negativamente o crescimento das microalgas, isso porque podem favorecer a formação de biofilmes incrustantes e corrosivos (ZERIOUH et al, 2017). Em cultivo de microalgas, o biofilme é formado nas paredes dos tubos ou das placas de fotobiorreatores, de modo que impedem a passagem da luz (BELOHLAV et al, 2020). Ademais, dependendo da espécie de microrganismo presente, pode haver a liberação de compostos que reduzem o crescimento das microalgas (WANG et al, 2013).…”

Section: Fatores Biológicos: Contaminação E Formação De Biofilmeunclassified

Produção de biomassa microalgal em escala: desafios e oportunidades

Santos¹,

Corrêa²,

Selesu³

et al. 2021

Microalgas: Potenciais Aplicações E Desafios

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Effect of hydrodynamics on the formation and removal of microalgal biofilm in photobioreactors

Cited by 21 publications

References 13 publications

Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms

Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms

Plasma‐activated liquids for mitigating biofilms on food and food contact surfaces

Produção de biomassa microalgal em escala: desafios e oportunidades

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