Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties

Parratt, Kirsten; Smerchansky, Madeline; Stiggers, Qwantayvious; Roy, Krishnendu

doi:10.1039/c7tb00896a

Cited by 12 publications

(7 citation statements)

References 38 publications

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“…13 Parratt et al investigated the effect of hydrogel composition on the human bone marrow stromal cell (hBMSC) differentiation into zone-specific cell phenotypes for the generation of human articular cartilage tissue. 27 This study showed that a composite multilayered material scaffold improved hBMSC differentiation into cartilage-like tissue by increasing sulfated glycosaminoglycans (sGAG) secretion and decreased collagen level. The sGAG is an important component in the cartilage ECM promoting mechanical strength of the scaffold.…”

Section: Recent Advances In Application Of Smart Biomaterialsmentioning

confidence: 96%

“…Biodegradable hydrogel systems typically make use of stimuli-specific cleavable cross-linkers, , which can be cleaved through hydrolysis, proteolysis, or disentanglement in response to stimuli . Parratt et al investigated the effect of hydrogel composition on the human bone marrow stromal cell (hBMSC) differentiation into zone-specific cell phenotypes for the generation of human articular cartilage tissue . This study showed that a composite multilayered material scaffold improved hBMSC differentiation into cartilage-like tissue by increasing sulfated glycosaminoglycans (sGAG) secretion and decreased collagen level.…”

Section: Recent Advances In Application Of Smart Biomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Smart Biomaterials: Recent Advances and Future Directions

Kowalski

Bhattacharya

Afewerki

et al. 2018

ACS Biomater. Sci. Eng.

148

107

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Smart biomaterials have the ability to respond to changes in physiological parameters and exogenous stimuli and continue to impact many aspects of modern medicine. Smart materials can promote promising therapies and improve treatment of debilitating diseases. Here, we describe recent advances in the current state-of-the-art design and application of smart biomaterials in tissue engineering, drug delivery systems, medical devices, and immune engineering.

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Section: Recent Advances In Application Of Smart Biomaterialsmentioning

confidence: 96%

Section: Recent Advances In Application Of Smart Biomaterialsmentioning

confidence: 99%

Smart Biomaterials: Recent Advances and Future Directions

Kowalski

Bhattacharya

Afewerki

et al. 2018

ACS Biomater. Sci. Eng.

148

107

View full text Add to dashboard Cite

show abstract

“…The middle-like layer is composed of 10% PEGDA + 10% CSMA in which Col X and sGAG are the highest. [191] HA-based hybrid scaffolds are made with the goal of inducing zonal-specific cellular morphology and ECM composition. Two layers of HA hydrogels enveloping specifically or randomly orientated PLA nanofibers are designed for SZ and MZ, whereas the gel with multiple vertical channels is developed for the DZ.…”

Section: Ecmmentioning

confidence: 99%

Recent Advances in “Functional Engineering of Articular Cartilage Zones by Polymeric Biomaterials Mediated with Physical, Mechanical, and Biological/Chemical Cues”

Dehghan-Baniani

Mehrjou

Chu

et al. 2023

Adv Healthcare Materials

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Articular cartilage (AC) plays an unquestionable role in joint movements but unfortunately the healing capacity is restricted due to its avascular and acellular nature. While cartilage tissue engineering has been lifesaving, it is very challenging to remodel the complex cartilage composition and architecture with gradient physio-mechanical properties vital to proper tissue functions. To address these issues, a better understanding of the intrinsic AC properties and how cells respond to stimuli from the external microenvironment must be better understood. This is essential in order to take one step closer to producing functional cartilaginous constructs for clinical use. Recently, biopolymers have aroused much attention due to their versatility, processability, and flexibility because the properties can be tailored to match the requirements of AC. This review highlights polymeric scaffolds developed in the past decade for reconstruction of zonal AC layers including the superficial zone, middle zone, and deep zone by means of exogenous stimuli such as physical, mechanical, and biological/chemical signals. The mimicked properties are reviewed in terms of the biochemical composition and organization, cell fate (morphology, orientation, and differentiation), as well as mechanical properties and finally, the challenges and potential ways to tackle them are discussed.

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“…Many native tissues contain gradients, such as cartilage and the osteochondral gradient between cartilage and bone. 134,135 As a result, biomaterial strategies have been developed to create gradients similar to tissues, including layer-bylayer [136][137][138][139] and 3D printing 140 strategies. Although there are three distinct layers in cartilage (superficial, middle, and deep zones), the challenge is to build many layers on top of one another to seamlessly transition from one zone to the next.…”

Section: Reviewmentioning

confidence: 99%