Effect of Hydrogen Peroxide and High Glucose on the Glucose Metabolism of Lymphoma-derived U937 Cells

Abstract

: Our study aimed to clarify speci c oxidative stress and glucose metabolic disorders in hemodialysis patients, by examining hydrogen peroxide H 2 O 2 -and high glucose-induced oxidative stress, glucose transport and the failure of glycolysis. As an in vitro blood cell model of end-stage renal disease ESRD in patients with diabetes, human monocytic U937 cells of malignant lymphoma origin were exposed to high glucose 28.9 mM for 6 days, with 5 mM H 2 O 2 added on the last day. The generation of intracellular reactive oxygen species ROS , glucose levels, lactate levels, AMP-activated protein kinase AMPK activity and Glut4 levels were examined. Exposure of U937 cells to H 2 O 2 resulted in a signi cant increase in intracellular ROS generation and glucose levels. Under high glucose conditions, treatment with H 2 O 2 signi cantly promoted these actions. In H 2 O 2 -induced U937 cells, AMPK activity and Glut4 levels were signi cantly increased, but lactate and pyruvate levels were signi cantly decreased. Thus, exposure of U937 cells to H 2 O 2 and a high glucose load promoted an increase in intracellular ROS, and exposure to H 2 O 2 induced increased glucose transport and high intracellular glucose due to reduced glycolytic metabolism. This suggests that reduced glycolytic metabolism might be induced in states of high oxidative stress in hemodialysis patients with diabetes.