Effect of hydrogen peroxide on antioxidant enzymes and metallothionein level in the digestive gland of Mytilus galloprovincialis

Cavaletto, Maria; Ghezzi, A.; Burlando, Bruno; Evangelisti, V.; Ceratto, N.; Viarengo, Aldo

doi:10.1016/s1532-0456(02)00030-3

Cited by 64 publications

(41 citation statements)

References 21 publications

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“…Cadmium (Cd) in particular can replace the essential metals zinc (Zn) and copper (Cu) in the composition of MTs and induce MT expression. Owing to their extremely high thiols content (about 30% of amino acids in their composition are represented by cystein), MTs could also be scavengers of reactive oxygen species (Cavaletto et al, 2002;Dondero et al, 2005). For bioindication purposes, the protein level of MTs is usually measured.…”

Section: Introductionmentioning

confidence: 99%

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Falfushynska¹,

Gnatyshyna²,

Gyori³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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The aim of the present study was to ascertain whether the metallothioneins (MTs) of freshwater mussels can serve as a biomarker of environmental quality in the spontaneously polluted area. Anodonta anatina mussels from an agricultural site (A), the cooling pond of a nuclear power plant (N) and a forestry as a reference site (F) in Western Ukraine were studied during spring, summer and autumn. Concentrations of copper (Cu), zinc (Zn) and cadmium (Cd) in MTs and in the tissue, MTs protein (MT-SH) level, oxidative stress indices and apoptotic activities were determined in the digestive gland. The three sites were clear distinguished by biomarkers. In the A-group, the specimens, despite higher concentrations of Cu and Zn in the tissue, had low level of MT-SH level (in two seasons) making the metal-keeping function of MTs invalid. MTs-bound metals dropped to about 2.3% of Cu, 0.4% of Zn and 9.1% of Cd in the tissue in autumn in this polluted area. Lower level of glutathione redox-index reflected the oxidative stress in this group. The N-group displayed high levels of MT-bound Cu (corresponding to up to 57.7% of the total tissue metal) and caspase-3 activity (up to 7 times higher than in the reference Fgroup), indicating specific for cooling pond pollution by Cu and thermal effects. In group F, the set of markers showed the characteristic of the reference site. An extremely low redox-index of glutathione (RI GSH: 0.45) was observed in the A-group in spring, and high levels of protein carbonyls and TBA-reactive substances in autumn in group N.Hence, multi-marker approach needed to provetoxicityassesments in mussel sexposed byspontaneous and mixed pollution.

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Section: Introductionmentioning

confidence: 99%

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Falfushynska¹,

Gnatyshyna²,

Gyori³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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“…It was found that MT levels in mammalian tissues under physiological conditions could be rather high to harbour important implications for MTs operation in Zn and redox metabolism (Capdevila et al, 1997;Capasso et al, 2005;Kelly et al, 2006). Different substances besides metals, such as fungicides fenhexamid, mancozeb, and also hydrogen peroxide, induce the elevation of MT content in fish (Viarengo et al, 1999;Cavaletto et al, 2002;Paris-Palacios et al, 2003;Mosleh et al, 2005;Kang, 2006). Inter-relation of elevated MT level with other stress proteins (catalase, GST) and negative relation to LPO products was confirmed for C. carassius over three seasons in a mixed polluted area characterized by spontaneous agricultural activities (Falfushynska et al, 2010a).…”

Section: Non-enzymatic Antioxidants: Glutathione and Other Scavengersmentioning

confidence: 77%

Environmental Pollution and Oxidative Stress in Fish

Stoliar¹,

Lushchak²

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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“…Significant changes in these parameters were observed at optimal treatment concentrations of 40 ppm formalin and 10 ppm H 2 O 2 . H 2 O 2 exposure stimulates lipid peroxidation, as H 2 O 2 biocidal effects in living organisms are based on production of free radicals (Siddique et al 2012;Cavaletto et al 2002). In addition, formaldehyde causes lipid peroxidation (Gulec et al 2006;Saito et al 2005) directly and via a secondary mechanism involving the production of reactive oxygen species (Hancock et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

et al. 2017

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Soft tunic syndrome (STS) is a protozoal disease caused by Azumiobodo hoyamushi in the edible ascidian Halocynthia roretzi. Previous studies have proven that combined formalin-hydrogen peroxide (H 2 O 2 ) bath is effective in reducing STS progress and mortality. To secure target animal safety for field applications, toxicity of the treatment needs to be evaluated. Healthy ascidians were bathed for 1 week, 1 h a day at various bathing concentrations. Bathing with 5-and 10-fold optimum concentration caused 100% mortality of ascidians, whereas mortality by 0.5-to 2.0-fold solutions was not different from that of control. Of the oxidative damage parameters, MDA levels did not change after 0.5-and 1.0-fold bathing. However, free radical scavenging ability and reducing power were significantly decreased even with the lower-than-optimal 0.5-fold concentration. Glycogen content tended to increase with 1-fold bathing without statistical significance. All changes induced by the 2-fold bathing were completely or partially restored to control levels 48 h post-bathing. Free amino acid analysis revealed a concentration-dependent decline in aspartic acid and cysteine levels. In contrast, alanine and valine levels increased after the 2-fold bath treatment. These data indicate that the currently established effective disinfectant regimen against the parasitic pathogen is generally safe, and the biochemical changes observed are transient, lasting approximately 48 h at most. Low levels of formalin and H 2 O 2 were detectable 1 h post-bathing; however, the compounds were completely undetectable after 48 h of bathing. Formalin-H 2 O 2 bathing is effective against STS; however, reasonable care is required in the treatment to avoid unwanted toxicity. Drug residues do not present a concern for consumer safety.

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Effect of hydrogen peroxide on antioxidant enzymes and metallothionein level in the digestive gland of Mytilus galloprovincialis

Cited by 64 publications

References 21 publications

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Environmental Pollution and Oxidative Stress in Fish

Biochemical changes and drug residues in ascidian Halocynthia roretzi after formalin–hydrogen peroxide treatment regimen designed against soft tunic syndrome

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