Effect of Hydrogen Peroxide Pretreatment on Physiological and Biochemical Variables during Germination of Alfalfa Seeds

Abstract

Background: Hydrogen peroxide is reactive oxygen species that plays role in plant response to biotic and abiotic stress. The pretreatment with hydrogen peroxide can confer an adaptive capacity for the plants in unpredictable environments. The alfalfa (Medicago sativa L.) is the legume more utilized in animal feeding in the world. Moreover, the alfalfa sprouts are known for the phytochemicals that promote health with antioxidant properties. Methods: This work aimed to determine the effect of hydrogen peroxide in the pretreatment process of alfalfa seeds on variables as total germination, speed of germination, activity and antioxidant enzymes. The alfalfa seeds were soaked for 12 h in the next treatments 0, 98, 294, 490, 784, 980 mM of hydrogen peroxide. Result: The results showed that total germination was higher with the hydrogen peroxide than with water except 980 mM. The results of the present research indicated that hydrogen peroxide had physiological and biochemical effects on the germination processes of alfalfa.

Efficacy of Priming Agents on Seed Germination and Plant Growth under Salt Stress in Vigna radiata

“…Chemical priming using salts like Sodium Chloride (NaCl), Potassium Chloride (KCl), Potassium Nitrate (KNO 3 ), Hydrogen Peroxide (H 2 O 2 ) etc. are known to be effective due to their involvement in the alleviation of abiotic stresses such as water deficit or salt stress by providing nutrients for growth or modulation of cytoplasmic activities under stress (Miladinov et al, 2019;Muñoz-Salinas, 2021). Organic priming agents such as extracts of Moringa leaves are known to contain metabolites like polyphenols, flavonoids and isothiocyanates, which afford defense against pathogens and UV rays (Ambreen et al 2021).…”