Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

Njie‐Mbye, Ya Fatou; Bongmba, Odelia Y.N.; Onyema, C. C.; Chitnis, A.S.; Kulkarni, Madhura; Opere, Catherine A.; LeDay, Angela M.; Ohia, Sunny E.

doi:10.1007/s11064-009-0085-7

Cited by 20 publications

(19 citation statements)

References 27 publications

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“…15 There is evidence that H 2 S donors can increase cyclic AMP production in isolated bovine and porcine retinae and retinal pigment epithelial cells, an effect that was dependent on endogenous biosynthesis of H 2 S and on the functional integrity of K ATP channels. 16,17 Taken together, these observations affirm the fact that H 2 S can have both physiological and pharmacological actions on ocular tissues.…”

Section: Introductionsupporting

confidence: 67%

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Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Salvi

Bankhele

Jamil

et al. 2016

Journal of Ocular Pharmacology and Therapeutics

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Purpose: In this study, we investigated the effect of a slow-releasing hydrogen sulfide (H 2 S) donor, GYY 4137, on intraocular pressure (IOP) in normotensive rabbits. Furthermore, we compared the IOP-lowering action of GYY 4137 with those elicited by other H 2 S-producing compounds, l-cysteine and ACS67 (a hybrid compound of latanoprost with an H 2 S-releasing moiety). Methods: IOP was measured in New Zealand normotensive male albino rabbits using a pneumatonometer (model 30 classic; Reichert Ophthalmic Instruments, Depew, NY). At 0 h, 50 mL of test compounds were applied topically to 1 eye of each animal, while the contralateral eye received the same quantity of vehicle (saline). IOP was measured hourly until baseline IOP readings were attained and animal eyes monitored for potential side effects (i.e., tearing, hyperemia). Results: GYY 4137 (0.1%-2%) produced a dose-dependent decrease in IOP reaching a maximum of 27.8% -3.14% (n = 5) after 6 h. Interestingly, a significant contralateral effect was observed in vehicle-treated controls eyes at all doses tested. l-cysteine (5%) and ACS67 (0.005%) also elicited a significant (P < 0.01) decrease in IOP that achieved a maximum of 28.84% -1.53% (n = 5) and 23.27% -0.51% (n = 5), respectively, after 3 h. All 3 H 2 S-producing compounds also caused a significant contralateral effect in vehicle-treated control eyes. Conclusion: We conclude that GYY 4137 and other H 2 S-producing donors can reduce IOP in normotensive rabbits. However, the profile of IOP-lowering action of GYY 4137 was different from the other H 2 S donors affirming its ability to act as a slow-releasing gas donor.

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Section: Introductionsupporting

confidence: 67%

“…For instance, H 2 S can alter neurotransmitter release from tissues of the anterior uvea and retina and can exert a neuroprotective action in the retina. 10,[15][16][17]19,27,28 There is evidence that H 2 S can relax both isolated mammalian irides and posterior ciliary arteries.…”

Section: Hydrogen Sulfide and Intraocular Pressure 373mentioning

confidence: 99%

Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Salvi

Bankhele

Jamil

et al. 2016

Journal of Ocular Pharmacology and Therapeutics

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“…Moreover the functional trans-sulfuration pathway in the retina proposes a protective action of this gas in this tissue. Studies from our laboratory have shown that H 2 S (using NaHS and Na 2 S as donors) can exert pharmacological effects in mammalian ocular tissues [23][24][25][26][27]. Although the enzymes involved in the biosynthesis of H 2 S are present in the eye, it is unclear whether they can generate this gas in situ.…”

Section: Discussionmentioning

confidence: 99%

“…In the eye, deficiency of CBS is often associated with many eye disorders including retinal degeneration, retinal detachment, optical atrophy and glaucoma [21,22]. Evidence from our laboratory have shown that H 2 S (using sodium hydrosulfide, NaHS and sodium sulfide, Na 2 S as donors) can relax ocular smooth muscle, regulate signal transduction processes and alter sympathetic and glutamergic neurotransmission in the anterior uvea and retina, an effect that is dependent on intramural biosynthesis of this gas [23][24][25][26][27]. Although enzymes involved in the biosynthesis of H 2 S are present in the eye and appear to be involved in mediating its pharmacological effects, to the best of our knowledge there are no reports of the basal levels of H 2 S in ocular tissues.…”

Section: Introductionmentioning

confidence: 99%

Endogenous Production of Hydrogen Sulfide in Isolated Bovine Eye

et al. 2011

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Hydrogen sulfide (H(2)S) is a novel gasotransmitter with physiological and pathological functions in vascular homeostasis, cardiovascular system and central nervous system. In the present study, we determined the endogenous levels of H(2)S in various tissues of the bovine eye. We also examined the basal levels of H(2)S in response to donors (sodium hydrosulfide, NaHS and sodium sulfide, Na(2)S), substrate (L: -cysteine), inhibitors (propargylglycine, PAG and aminooxyacetic acid, AOA) and activator (S-adenosyl-L: -methionine, SAM) of this gas in the bovine retina. H(2)S was measured using a well established spectrophotometric method. The highest concentration of endogenous H(2)S was detected in cornea (19 ± 2.85 nmoles/mg protein, n = 6) and retina (17 ± 2.1 nmoles/mg protein, n = 6). Interestingly, H(2)S was not present in vitreous humor. The inhibitors of CSE and CBS; PAG (1 mM) and AOA (1 mM), significantly attenuated the production of H(2)S in the bovine retina by 56.8 and 42%, respectively. On the other hand the activator of CBS; SAM (100 μM), H(2)S donors; NaHS (1 μM) and Na(2)S (100 μM), significantly increased endogenous levels of H(2)S in bovine retina. L: -cysteine (10-300 μM) produced a significant (P < 0.05) concentration-dependent increase in H(2)S levels reaching a maximal at 300 μM. We conclude that H(2)S is endogenously produced in various tissues of the isolated bovine eye. Moreover, endogenous levels of H(2)S are enhanced in the presence of substrate (L: -cysteine), an activator of CBS (SAM) and H(2)S donors but are blocked by inhibitors of enzymes that synthesize this gas in neural retina.

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“…In the posterior segment of the eye, it regulates cyclic adenosine monophosphate (cAMP) production and prejunctional excitatory neurotransmission in porcine and bovine retina (26). Moreover, ACS67, an H 2 S-releasing molecule, demonstrated a significant ocular hypotensive activity in glaucomatous rabbits and an elevation of reduced glutathione levels (27) which suggest a potential therapeutic application for H 2 S donors in ocular hypertension and neuropathies.…”

Section: Introductionmentioning

confidence: 99%

In Vitro-Controlled Release Delivery System for Hydrogen Sulfide Donor

2014

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Abstract. Hydrogen sulfide (H 2 S) is having many potential pharmacological and physiological actions which reported that therapeutically useful concentration is low (100-160 μM) and a higher concentration could be toxic. Most of its donors produce it on coming into contact with water. All of these problems could be solved by a controlled-release delivery system which does not utilize water in any of its development steps. Therefore, 12 sustained release formulations were prepared by dissolving sodium hydrogen sulfide (NaHS)-a model H 2 S donor-in polymer solutions, prepared by dissolving polymers (consisted of either polylactide (PLA) or polylactide co-glycolide (PLGA), containing free carboxylic acid or capped allyl ester end group) in a mixture of benzyl benzoate (BB) and benzyl alcohol (BA). The formulation was injected in simulated tear fluid (STF) from which samples were withdrawn at specified times and assayed for NaHS content. We found decrease in burst and overall release with increase in polymer concentration from 10 to 20% w/v. The formulations containing free end group showed significant (p<0.05) reduction of burst release (11% vs 21%). However, the overall release or the average amount released per hour was found to be significantly (p<0.05) increased for formulations containing polymers with free end group than those with capped end group. A sustained level of H 2 S was found to be maintained for 72 h which should be further increased to a month to make it a viable H 2 S donor delivery system in addition to investigating toxicity profile specifically for the purpose of subconjunctival ocular delivery.KEY WORDS: controlled release; hydrogen sulfide; hydrogen sulfide donor; in situ gel forming; phase sensitive; smart polymer.

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Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

Cited by 20 publications

References 27 publications

Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Endogenous Production of Hydrogen Sulfide in Isolated Bovine Eye

In Vitro-Controlled Release Delivery System for Hydrogen Sulfide Donor

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