Effect of hydroxyapatite as a component of biostable composites on population and proliferation of mesenchymal stem cells

Abstract

Three groups of biostable composite materials were studied. The initial binder polymers (polymethylmethacrylate, polyamide-12, superhigh-molecular-weight polyethylene) and hydroxyapatite-containing composites on the basis of these polymers were tested. Biostable polymers, including those containing hydroxyapatite, were nontoxic for fibroblasts and mesenchymal stem cells: the adhesion parameters for these cells were maximum for polyamide-12 and superhigh-molecular-weight polyethylene and did not depend on the presence of hydroxyapatite. Cell adhesion to "pure" polymethylmethacrylate was significantly lower than to other composites, but increased after integration of hydroxyapatite. The efficiency of proliferation of fibroblast and mesenchymal stem cell on the surface of polyamide-12 and superhigh-molecular-weight polyethylene was maximum and did not depend on the presence of hydroxyapatite. The efficiency of cell proliferation on the surface of "pure" polymethylmethacrylate was low, but increased significantly if it was combined with hydroxyapatite, particularly in areas of mineral particles accumulation. It seems that the presence of high amounts of hydroxyapatite in polymethylmethacrylate samples promotes cell adhesion and proliferation.

Improved osteogenesis of human adipose-derived stromal cells on hydroxyapatite-mineralized graphene film

“…To initiate the mineralization of HAp, which induce stimulation for osteogenic differentiation of cells, the UVO functionalized oxygen-atoms on GR serves as anchor sites for calcium cations (Ca 2+ ) in SBF to electrostatically interact and form nuclei which then attracts phosphate anions (HPO 4 2− ) to eventually aggregate into HAp crystals over time [29], and GR_UVO5min providing synergistic effects from both increased surface wettability (figure 2) and bioactive stimulation of needle-like HAp crystals was selected for cell cultivation (figure 3). When the HAp/GR was applied to the hASCs, hASCs proliferation was increased in HAp/GR (figures 4(A)-(C)), which may be due to the effect of HAp-stabilized on GR [30]. Cell viability and adherence were not different in all groups (figures 4(D)-(F)), based on these results, HAp/GR was shown to be safe and suitable for hASCs.…”

Ceramic and Osteoceramic Implants: Upcoming Trends

“…По литературным данным [3,4,15,17,31,32,37,39], современный имплантат должен быть пористым, достаточно прочным для скелетного использования, биосовместимым и способствовать остеокондукции. Идеальный имплантат должен быть композитным, дополненным какимлибо агентом (рекомбинантными костными морфогенетическими белками, факторами роста или стволовыми клетками), придающим материалу индуктивные свойства [1,6,15,17,26,28,30,38,41].…”

“…Одной из причин потери трудоспособности являются заболевания и посттравматические состояния, сопряженные с необходимостью реконструкции и замещения участков костной ткани, протезирования фрагментов опорно-двигательного аппарата. С точки зрения биохимической совместимости с организмом, при протезировании наиболее предпочтительны материалы, относящиеся к классу керамик [2,6,10,11,16,17,26,27,31,33,34,36].…”

“…Наиболее часто используемые керамики в группе кальцийфосфатных керамик -гидроксиапатит (ГАП) и β-трикальцийфосфат (β-ТКФ), являющиеся неорганическим компонентом костей позвоночных [3,4,8,10,11,14,16,17,20,21,26,29,33,34,37,39,40]. Использование иттриево-циркониевой и алюминиевой керамик связано с их высокими механическими свойствами [2,7,9,11,19,36].…”

Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phase

“…They are located primarily in the bone marrow and are characterised by a capacity to differentiate into mesenchymal lineages such as bone, tendon, fat, and cartilage [8][9][10]. In general, during the bone healing process, MSCs migrate into the impaired bone area, wherein they proliferate, differentiate, and thus may support stabilisation of implanted materials [11][12][13]. Any failure in mobilisation, proliferation, and differentiation of these progenitor cells may lead to failure in new bone formation.…”