Effect of Hydroxyapatite Coating Surface Morphology on Adsorption Behavior of Differently Charged Proteins

He, Zhihuan; Sun, Shili; Deng, Chunlin

doi:10.1007/s42235-020-0028-1

Cited by 22 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, to date, the effect of functional surfaces on enzyme immobilization has rarely been reported because of the lack of microscopic research from experiments. Recently, some experimental methods have been employed to study the protein–surface interaction, i.e., circular dichroism (CD) spectroscopy, single-molecule force spectroscopy (SMFS), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), , Fourier transform infrared spectroscopy (FTIR), , and sum frequency generation (SFG) vibrational spectroscopy . However, the adsorption behavior (i.e., orientation and conformation) of proteins on surfaces at the atomistic level is very hard to obtain from experiments.…”

Section: Introductionmentioning

confidence: 99%

Electrostatic Effect of Functional Surfaces on the Activity of Adsorbed Enzymes: Simulations and Experiments

Zheng¹,

Yang

Zheng³

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The efficient immobilization of haloalkane dehalogenase (DhaA) on carriers with retaining of its catalytic activity is essential for its application in environmental remediation. In this work, adsorption orientation and conformation of DhaA on different functional surfaces were investigated by computer simulations; meanwhile, the mechanism of varying the catalytic activity was also probed. The corresponding experiments were then carried out to verify the simulation results. (The simulations of DhaA on SAMs provided parallel insights into DhaA adsorption in carriers. Then, the theory-guided experiments were carried out to screen the best surface functional groups for DhaA immobilization.) The electrostatic interaction was considered as the main impact factor for the regulation of enzyme orientation, conformation, and enzyme bioactivity during DhaA adsorption. The synergy of overall conformation, enzyme substrate tunnel structural parameters, and distance between catalytic active sites and surfaces codetermined the catalytic activity of DhaA. Specifically, it was found that the positively charged surface with suitable surface charge density was helpful for the adsorption of DhaA and retaining its conformation and catalytic activity and was favorable for higher enzymatic catalysis efficiency in haloalkane decomposition and environmental remediation. The neutral, negatively charged surfaces and positively charged surfaces with high surface charge density always caused relatively larger DhaA conformation change and decreased catalytic activity. This study develops a strategy using a combination of simulation and experiment, which can be essential for guiding the rational design of the functionalization of carriers for enzyme adsorption, and provides a practical tool to rationally screen functional groups for the optimization of adsorbed enzyme functions on carriers. More importantly, the strategy is general and can be applied to control behaviors of different enzymes on functional carrier materials.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrostatic Effect of Functional Surfaces on the Activity of Adsorbed Enzymes: Simulations and Experiments

Zheng¹,

Yang

Zheng³

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…This could also be influenced by electrostatic interactions between the positively charging QAS and the negatively charging hydroxyapatite. 70…”

Section: Resultsmentioning

confidence: 99%

Janus functional electrospun polyurethane fibrous membranes for periodontal tissue regeneration

Lin,

He,

et al. 2023

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…This may be contributed by the electrostatic interaction between positively charged quaternary ammonium salts and negatively charged hydroxyapatite. 63 At the same time, a rough surface is more conducive to mineral deposition. It should be noted that the PU-BE sample was not analyzed because it became sticky and could not maintain the membrane structure after 7 days of immersion in simulated body fluids (Fig.…”

Section: Resultsmentioning

confidence: 99%

A bioinspired Janus polyurethane membrane for potential periodontal tissue regeneration

Wang

et al. 2022

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

Effect of Hydroxyapatite Coating Surface Morphology on Adsorption Behavior of Differently Charged Proteins

Cited by 22 publications

References 25 publications

Electrostatic Effect of Functional Surfaces on the Activity of Adsorbed Enzymes: Simulations and Experiments

Electrostatic Effect of Functional Surfaces on the Activity of Adsorbed Enzymes: Simulations and Experiments

Janus functional electrospun polyurethane fibrous membranes for periodontal tissue regeneration

A bioinspired Janus polyurethane membrane for potential periodontal tissue regeneration

Contact Info

Product

Resources

About