Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells

Nie, Xiaowei; Sheng, Wenjie; Hou, Daorong; Liu, Qiang; Wang, Ronggen; Tan, Yong

doi:10.1016/j.cca.2018.05.004

Cited by 26 publications

(16 citation statements)

References 41 publications

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“…These results indicate that ICA can promote the biosynthesis of E 2 by stimulating aromatase expression in a concentration-dependent manner. This conclusion is supported by previous studies in human ovarian granulosa cells (KGN cells), human osteoblastic cells (UMR-106 cells), rat ovarian granulosa cells, and TGF-β1-treated rat Leyding cells that showed that ICA promotes E 2 biosynthesis by enhancing aromatase mRNA and protein expression in a concentration-dependent manner (Yang et al, 2013(Yang et al, , 2016Nie et al, 2018). In this study, to investigate the potential mechanism by which ICA regulates aromatase expression, we focused on proteins in the cAMP/PKA/CREB pathway because CYP19 expression in ovaries is mediated by the classical cAMP/PKA/CREB pathway (Gore-Langton and Dorrington, 1981;Guiguen et al, 2010).…”

Section: Discussionsupporting

confidence: 73%

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

Zheng

Liu

et al. 2020

Front. Mar. Sci.

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In Eriocheir sinensis aquaculture, crabs feeding on an artificial diet without steroid hormones available in ice-fresh fish or snails show delayed ovarian maturation and late listing. Icariin (ICA), a safe plant-derived monomer compound, can effectively promote estradiol (E 2) synthesis and ovarian development in mammals, but its effect in crustaceans has not been reported. We investigated the effects of ICA on estrogen synthesis, vitellogenesis, and oocyte development in Chinese mitten crabs fed with one of four diets containing 0, 50, 100, and 200 mg/kg ICA, respectively, for 8 weeks. Results showed that vitellogenin (VTG) contents, gonadosomatic indexes (GSIs), and oocyte volumes in ICA50 and ICA 100 crabs were significantly higher than those in the control. The highest upregulated expression of VTG in hepatopancreases and ovaries was detected in ICA100 crabs. Hemolymph E 2 contents were also significantly increased in ICA100 crabs. Enzyme-linked immunosorbent assay (ELISA) and western blot analysis indicated that the increase in E 2 concentrations was attributable to an elevation in aromatase protein levels in ovaries through the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP response element binding (CREB) protein pathway. These findings suggest that ICA could serve as a natural additive to resolve the problem of delayed ovarian maturation of crabs raised in ponds.

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Section: Discussionsupporting

confidence: 73%

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

Zheng

Liu

et al. 2020

Front. Mar. Sci.

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“…It has been found to have both androgen-like and estrogen-like effects ( Zhang and Yang, 2006 , Wang et al., 2009 ). Some of the ingredients of TFE, such as hyperin and icariin, were confirmed to promote the secretion of E 2 and progesterone through the upregulation of CYP17 and CYP19 in the ovary granulosa cells of rats in vitro ( Nie et al., 2019 ). The results obtained in this study showed that TFE had the ability to improve the secretion of endogenous E 2 , which in turn promoted the development of the fallopian tube via ER receptors.…”

Section: Discussionmentioning

confidence: 99%

Improving effects of Epimedium flavonoids on the selected reproductive features in layer hens after forced molting

Huo

Guo

et al. 2020

Poultry Science

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In the present study, for the purpose of investigating the effects of the total flavonoids of Epimedium ( TFE ) in regard to preventing the development of atrophied oviducts and follicles induced by forced molting, 300-day-old Hy-Line Brown layer hens were divided into 3 study groups as follows: the control ( CON ) group was the normal group, without forced molting and TFE treatments; the TFE1 group was treated by adding a 1‰ TFE treatment after forced molting; and the TFE0 group was not treated by TFE after forced molting. During this study's experimental process, the egg production rates were recorded each day. In addition, the hens were randomly chosen to be weighed every 4 D and also randomly selected to be sacrificed every 7 D. Then, sample tissues of albumen-secreting part and uterus from the fallopian tube of the layer hens were collected for PCR and hematoxylin-eosin staining tests. The results showed that the body weights, number of follicles, and weights and sizes of the fallopian tube for the TFE1 and TFE0 groups were significantly reduced when compared with those of the control group on the 15th D of the experiment. Furthermore, at the end of study, it was found that the egg production rates, weights of the fallopian tube, and ovarian follicles of TFE1 had recovered to normal levels. At the same time, the serum estrogen and the expressions of the progesterone receptor and estrogen receptor mRNA in fallopian tube were higher than those observed for the TFE0 group. The results of this study provided valuable evidence that TFE could improve the development of atrophied oviducts and increase the egg laying rates, thereby making it a potential multicomponent natural drug for egg production in the future.

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“…HR is a flavonoid compound [ 36 ], also known as quercetin-3-O-beta-D-galactoside [ 37 ], and it can improve the endocrine function of the ovary [ 38 ]. A previous study has reported that HR promotes the proliferation and secretion of estrogen and progesterone in rat ovarian granulosa cells, thereby improving ovarian endocrine function [ 39 ]. However, the underlying molecular mechanisms need to be comprehensively elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Estrogen is mainly produced in granulosa cells during follicular development and produces negative feedback on luteinizing hormone [ 44 ]. Progesterone is synthesized and secreted by follicles and corpus luteum components of mammalian ovary, which can affect the function of granulosa cells during follicular development before ovulation [ 39 ]. TSC1 is an important upstream negative regulator of mTORC1.…”

Section: Discussionmentioning

confidence: 99%

Hyperin Alleviates Triptolide-Induced Ovarian Granulosa Cell Injury by Regulating AKT/TSC1/mTORC1 Signaling

You

Cao

Cheng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Premature ovarian insufficiency (POI) is characterized by the loss of ovarian function before 40 years of age and affects approximately 1% of women worldwide. Caragana sinica is a traditional Miao (a Chinese ethnic minority) medicine that improves ovarian function and follicular development. In the present study, we aimed to investigate the effect of active ingredients of C. sinica on POI and determine underlying mechanisms. Herein, the chemical composition of the C. sinica compound was analyzed using ultra-high-performance liquid chromatography, which identified hyperin (HR) as one of the main ingredients in C. sinica. Then, interaction targets of HR and POI were predicted and analyzed using network pharmacology and bioinformatics. The effect of HR on triptolide (TP)-induced granulosa cell injury was evaluated, and the underlying mechanism was explored based on bioinformatic results. A total of 100 interaction targets for POI and HR were obtained. The protein-protein interaction network of identified interaction targets emphasized the topological importance of AKT1. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that HR might regulate POI by modulating the mechanistic target of rapamycin (mTOR) signaling pathway. In addition, the KEGG graph of the mTOR signaling pathway revealed that AKT phosphorylation inhibits the TSC1/2, while TSC1/2 activation inhibits the expression of mTORC1. The fundamental experiment revealed that HR increased proliferation, progesterone receptor levels, and estradiol levels decreased by TP in KGN cells. Additionally, HR alleviated TP-induced apoptosis and G1/G1 phase arrest in KGN cells. Western blotting demonstrated that HR increased the phosphorylation of AKT and mTORC1 and decreased TSC1 expression in TP-induced KGN cells. Collectively, our findings revealed that HR alleviates TP-induced granulosa cell injury by regulating AKT/TSC1/mTORC1 signaling, providing insight into the treatment of POI.

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Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells

Cited by 26 publications

References 41 publications

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

Improving effects of Epimedium flavonoids on the selected reproductive features in layer hens after forced molting

Hyperin Alleviates Triptolide-Induced Ovarian Granulosa Cell Injury by Regulating AKT/TSC1/mTORC1 Signaling

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