Effect of hyperoxia on the intestinal IgA secretory component in neonatal rats and on intestinal epithelial cells in vitro

Dy, Liu; Jj, Li

doi:10.1590/s0100-879x2010007500106

Cited by 15 publications

(13 citation statements)

References 29 publications

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“…However, few studies have reported changes in pulmonary immunity induced by hyperoxia. Our previous studies had demonstrated that moderate oxygen induced an increase and hyperoxia induced a decrease of intestinal SC in vivo and in vitro [13] and that ileal SIgA was remarkably increased in neonatal rats under conditions of hyperoxia [14]. In the present work, neonatal rats were under the hyperoxia condition for 1st, 3rd, 5th, 7th, and 10th days, respectively.…”

Section: Introductionmentioning

confidence: 62%

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Effect of Hyperoxia on Pulmonary SIgA and Its Components, IgA and SC

et al. 2013

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PurposeOxygen therapy (hyperoxia) is essential for the treatment of some neonatal critical care conditions. The lung is the primary target for the changes induced by hyperoxia. Secretory immunoglobulin A (SIgA), IgA and secretory component (SC) reflect the local immunity in the respiratory tract induced by hyperoxia.MethodsThe enzyme-linked immunosorbent assay, immunohistochemistry staining, Western blot and Real-time PCR were used to detect the levels of cytokines, IgA and SIgA in bronchoalveolar lavage as well as IgA and SC/pIgR in pulmonary tissue.ResultsThe levels of IgA and SIgA in BAL fluid were gradually increased following neonatal rat development. Compared with air-inhaling group, in the hyperoxia group IgA, SIgA and other cytokines except IL-1 in BAL fluid were significantly elevated on the 3rd, 5th and 7th days, but on the 10th day TNF-α, SIgA and IgA rapidly decreased. In the hyperoxia group, both the protein expression of SC/pIgR and the mRNA expression of SC/pIgR were remarkably increased on the 3rd, 5th and 7th days, but were significantly decreased on the 10th day, respectively. Conclusion: The large amount of SIgA, IgA and SC in the early period of hyperoxia might protect the lungs of the neonatal rats against acute pulmonary injury, however, in the late period of hyperoxia, the abruptly drop of SIgA and its component might lead to pulmonary immunity abnormality. In hyperoxia, the increased expression of cytokines might contribute to the expression of IgA and SC.

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Section: Introductionmentioning

confidence: 62%

“…Animal model was built as described previously [13]. Briefly, within 12 hours of birth, all pups were randomly divided into air-inhaling group (group that was exposed to room air, FiO 2 = 0.21, N = 68) and hyperoxia group (group that was exposed to 90 ~ 95 % O2, FiO 2 = 0.9 ~ 0.95, N = 203).…”

Section: Methodsmentioning

confidence: 99%

Effect of Hyperoxia on Pulmonary SIgA and Its Components, IgA and SC

et al. 2013

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“…Although hyperoxia is an indispensable treatment in clinical critical care for various neonatal diseases, the neonatal small intestine is highly sensitive to oxygen (30). The structure of intestinal villi in neonatal rats was destroyed in the hyperoxic environment, which may have affected the barrier function of their intestines, making them susceptible to bacterial insult (33). Culturing of intestinal epithelial cells in vitro showed that hyperoxia could inhibit cell growth, and destroy intestinal epithelial cells, which would promote the invasion of intestines by bacteria (33).…”

Section: Discussionmentioning

confidence: 99%

Involvement of nuclear factor erythroid 2‑related factor�2 in�neonatal intestinal interleukin‑17D expression in hyperoxia

et al. 2020

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Interleukin 17d (IL-17d) plays an important role in host defense against inflammation and infection. In the present study, the role of nuclear factor erythroid 2-related factor 2 (Nrf2) in regulating the production of IL-17D was investigated under hyperoxia. For this purpose, neonatal rats were randomized into two groups; the model group was exposed to hyperoxia (80-85% O 2), while the control group was maintained under normoxic conditions (21% O 2). Small intestine tissue was collected on postnatal days 3, 7, 10 and 14. IL-17D expression was detected by immunofluorescence, immunohistochemistry and western blotting. The levels of Nrf2 and kelch-like ECH-associated protein 1 (keap1) were detected by immunohistochemistry and western blotting. Results showed that IL-17D expression in intestine epithelial cells increased steadily, reaching a peak on day 7, and decreased gradually on days 10 and 14 under hyperoxia. Nrf2 expression was consistent with IL-17D, and it was positively correlated with IL-17D. However, on postnatal days 10 and 14, the number of CD4 + T cells and CD19 + B cells expressing IL-17D was increased, and positive cells of the model group were significantly more than that of the control group. Keap1 levels were lower at the early stage. In conclusion, the expression levels of intestinal IL-17D and Nrf2 were altered simultaneously following neonatal rat development in hyperoxia, indicating that Nrf2 may be involved in regulating the expression of IL-17d in intestinal epithelial cells. Moreover, IL-17D in intestinal epithelial cells may play a unique immunological role during hyperoxia.

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“…HIF-1α may also provide information on hypoxia induction and oxidative stress ( 12 ). Long-term treatment with hyperoxia may exert serious toxic effects on intestinal epithelial cells in vitro and in vivo ( 2 , 13 – 16 ). Therefore, the aim of the prsent study was to investigate the cause of intestinal injury during hyperoxia, and determine whether ROS generation is a main factor in intestinal injury under hyperoxic conditions.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia

et al. 2017

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Long-term therapeutic hyperoxia may exert serious toxic effects on intestinal epithelial cells in vitro and in vivo. The aim of the present study was to investigate the cause of this intestinal injury under conditions of hyperoxia. Caco-2 cells were treated with different concentrations of hydrogen peroxide (H2O2) and 85% hyperoxia for 24 h. Higher rates of injury of Caco-2 cells were observed in the hyperoxia and H2O2 groups compared with the control group. The reactive oxygen species (ROS) level of the hyperoxia group was significantly higher compared with that of the 400 μM H2O2 group. The protein and gene levels of RelA, RelB, hypoxia-inducible factor-1α, tumor necrosis factor-α and apoptosis signal-regulating kinase 1 were significantly higher in the hyperoxia and H2O2 groups compared with those in the control group. In conclusion, during hyperoxia, intestinal epithelial cells were destroyed and the levels of ROS were increased. Therefore, ROS may play an important role in intestinal injury in a hyperoxic environment.

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Effect of hyperoxia on the intestinal IgA secretory component in neonatal rats and on intestinal epithelial cells in vitro

Cited by 15 publications

References 29 publications

Effect of Hyperoxia on Pulmonary SIgA and Its Components, IgA and SC

Effect of Hyperoxia on Pulmonary SIgA and Its Components, IgA and SC

Involvement of nuclear factor erythroid 2‑related factor�2 in�neonatal intestinal interleukin‑17D expression in hyperoxia

Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia

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