Effect of hyperthermia on cell viability, amino acid transfer, and
                    milk protein synthesis in bovine mammary epithelial cells

Zhou, Jia; Yue, Shungming; Yue, Benchu; Wang, Zhisheng; Wang, Lizhi; Peng, Quanhui; Hu, Rui; Xue, Bai

doi:10.5187/jast.2021.e128

Cited by 3 publications

(3 citation statements)

References 63 publications

(83 reference statements)

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“…Our results show that HS similarly impacts both differentiated (D) and undifferentiated (UD) cells, disrupting protein folding and RNA metabolism and downregulating solute carrier family 7, member 5 ( SLC7A5 ), an essential amino acid transporter for milk synthesis 44,45 . Downregulation of SLC7A5 was also observed in undifferentiated MAC-T cells 15 and in dairy cows 10 under HS. In addition, we found that HS repressed transfer RNA cysteine ( TRNAC - ACA ) and BarH like homeobox 1 ( BARHL1 ), which are related to milk yield and fat percentage, respectively 46,47 .…”

Section: Discussionmentioning

confidence: 78%

“…Previous studies using MAC-T cells under HS conditions have primarily focused on their undifferentiated state. For example, expression of HSP genes and apoptosis-related genes was significantly upregulated in undifferentiated MAC-T after HS treatment 14 , whereas baseline expression of milk protein genes was downregulated 15 . Recent work employing RNA-seq and methylated RNA immunoprecipitation sequencing (MeRIP) profiled transcriptomics and N6-methyladenosine (m6A) modification of HS undifferentiated MAC-T cells 16 , revealing upregulated expression of heat shock factor 1 ( HSF1 ), heat shock protein 70 ( HSP70 ), and apoptosis-related genes, and altered m6A modifications within genes in TGFβ, Wnt, and Hippo signaling pathways.…”

Section: Introductionmentioning

confidence: 98%

“…Recent work employing RNA-seq and methylated RNA immunoprecipitation sequencing (MeRIP) profiled transcriptomics and N6-methyladenosine (m6A) modification of HS undifferentiated MAC-T cells 16 , revealing upregulated expression of heat shock factor 1 ( HSF1 ), heat shock protein 70 ( HSP70 ), and apoptosis-related genes, and altered m6A modifications within genes in TGFβ, Wnt, and Hippo signaling pathways. Currently, the impacts of HS on lactogenic MAC-T cells and milk synthesis pathways remains poorly understood, and few potent regulators or pathways have been identified 14,15 .…”

Section: Introductionmentioning

confidence: 99%

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Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells

Yu,

Harman,

Danev

et al. 2024

Preprint

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Heat stress (HS) in cattle significantly challenges the dairy industry by reducing milk production. However, the molecular mechanism behind mammary gland responses to HS and recovery remains poorly understood. This study aimed to determine the transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells after HS and post-HS recovery. Six culture conditions were analyzed: MAC-T cells cultured in basal medium, lactogenic medium to induce differentiation, differentiated cells at standard temperature (37C;) or HS (42C;) for 1 hour. HS cells were collected after incubation at 37C; for either 2 or 6 hours to examine the extent of recovery. A total of 1,668 differentially expressed genes (DEGs) were identified. Differentiated cells expressed genes associated with milk lipid synthesis, indicating lactogenic potential. HS suppressed genes involved in cellular differentiation and activated heat shock protein genes. Several transcription factors were identified as potential regulators of HS response. During recovery, chaperon-mediated protein folding remained elevated, apoptosis regulation genes were induced at 2 hours, and cellular homeostasis regulation genes were enriched at 6 hours. Overall, these findings provide a foundation for the molecular mechanisms involved in HS in cattle.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells

Yu,

Harman,

Danev

et al. 2024

Preprint

View full text Add to dashboard Cite

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Effects of L-leucine and sodium acetate on milk protein synthesis under heat stress conditions in bovine mammary epithelial cells in vitro

Lim,

Lee,

Nejad

et al. 2024

Journal of Thermal Biology

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Study on the current research trends and future agenda in animal products: an Asian perspective

Lee,

Mariano

et al. 2023

J Anim Sci Technol

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This study aimed to analyze the leading research materials and research trends related to livestock food in Asia in recent years and propose future research agendas to ultimately contribute to the development of related livestock species. On analyzing more than 200 relevant articles, a high frequency of studies on livestock species and products with large breeding scales and vast markets was observed. Asia possesses the largest pig population and most extensive pork market, followed by that of beef, chicken, and milk; moreover, blood and egg markets have also been studied. Regarding research keywords, “meat quality” and “probiotics” were the most common, followed by “antioxidants”, which have been extensively studied in the past, and “cultured meat”, which has recently gained traction. The future research agenda for meat products is expected to be dominated by alternative livestock products, such as cultured and plant-derived meats; improved meat product functionality and safety; the environmental impacts of livestock farming; and animal welfare research. The future research agenda for dairy products is anticipated to include animal welfare, dairy production, probiotic-based development of high-quality functional dairy products, the development of alternative dairy products, and the advancement of lactose-free or personalized dairy products. However, determining the extent to which the various research articles’ findings have been applied in real-world industry proved challenging, and research related to animal food laws and policies and consumer surveys was lacking. In addition, studies on alternatives for sustainable livestock development could not be identified. Therefore, future research may augment industrial application, and multidisciplinary research related to animal food laws and policies as well as eco-friendly livestock production should be strengthened.

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Effect of hyperthermia on cell viability, amino acid transfer, and milk protein synthesis in bovine mammary epithelial cells

Abstract: Effect of hyperthermia on cell viability, amino acid transfer, and milk protein synthesis in bovine mammary epithelial cells

Cited by 3 publications

References 63 publications

Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells

Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells

Effects of L-leucine and sodium acetate on milk protein synthesis under heat stress conditions in bovine mammary epithelial cells in vitro

Study on the current research trends and future agenda in animal products: an Asian perspective

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