Effect of hyperthermia on experimental choroidal melanoma.

Abstract

SUMMARY Thirty-five rabbit eyes were implanted subchoroidally with Greene's hamster melanoma. When the tumours reached a base diameter of 5 mm, they were treated with ultrasonically induced hyperthermia with a range of temperatures and exposure durations (43-67°C and 75 s to 60 min). Of the 23 treated eyes examined two months after treatment eight showed complete regression of the tumour. Seven showed initial tumour regression, but there was subsequent regrowth of tumour round the margins of the original mass. In eight eyes the tumour continued to grow, though in some cases the rate of growth appeared to be slower than in the controls. In contrast, in all untreated animals the tumour grew to fill the vitreous cavity. These preliminary findings indicate that ultrasonically induced hyperthermia can be an effective local treatment of this intraocular tumour model.The tumoricidal effect of hyperthermia either alone or in conjunction with radiotherapy is well known.' Heat treatment of tumours has ranged from the use of hot waterbaths2 to regional perfusion with warmed blood.3 Modern methods of inducing selective heating of tissues has involved radiofrequency electromagnetic waves,4 microwaves,56 and ultrasound techniques.78 This paper describes local, ultrasonically induced hyperthermia (UIH) in the treatment of experimental choroidal melanoma. We describe the model and define temperature and exposure duration relationships required to kill Greene's hamster melanoma growing in the choroid of rabbits. Material and methodsThirty-five eyes of 26 rabbits (New Zealand White and Dutchbelt strains weighing between 2 and 3 kg) were studied.An experimental model of choroidal melanoma was prepared by a modification of Krohn and colleagues' technique.9 The tumour stock used was a strain of amelanotic Greene's melanoma"' maintained by serial transplantation into the anterior Correspondence to

High intensity focused ultrasound—potential for cancer treatment

“…The first group of sites includes the following: bladder [17,37]; breast; kidney [17]; sub-costal liver [17,37]; prostate, with access either transrectally [38] or suprapubically [17,39]; and eye [40]. Surgical removal, temporary or otherwise, of overlying bone has been suggested as a clinically acceptable preliminary to gaining access for HIFU to otherwise inaccessible liver pathology and also to focal brain pathology.…”

Termoterapia transpupilar como opção terapêutica para hemangioma circunscrito de coróide: relato de caso

“…Em contraste à fotocoagulação a laser, na hipertermia os efeitos produzidos pela coagulação estão ausentes, otimizando a penetração do calor quando realizada apropriadamente (7)(8) . Isto se deve ao fato que as camadas superficiais da retina não são afetadas na TTT, permitindo que a energia luminosa chegue até as camadas mais profundas.…”

Das subchorioidal implantierte Greene-Melanom: Ein tierexperimentelles Modell für das maligne Aderhautmelanom

“…In 3 Fallen setzte das Tumorwachstum nicht oder verzogert em, weshalb diese Augen ausgeschlossen werden mul3ten. Die von anderen Autoren beschriebene gute Angehrate des Greene-Melanoms in der Aderhaut des Kaninchenauges konnte somit auch durch die vorliegende Studie bestatigt werden (6,(11)(12)(13)(14).…”

“…Obwohl das Greene-Melanom em ursprunglich spontan im syrischen Hamster vorkommender Tumor der Haut ist, sind seine morpho!ogischen Charakteristika in vieler Hinsicht dem epitheloidzelligen mensch!ichen Aderhautmelanom vergleichbar (8)(9)(10) Analog dem Vorgehen anderer Arbeitsgruppen (6,11,12,14) wurden in unserer Versuchsreihe Tumorgewebestucke implantiert. Albert und Mitarbeiter (2) In einer tierexperimentellen Studie gelang es erstmals 1980, menschliche, maligne Melanomzellen in die Augenvorderkammer von Mäusen mit kongenitalem Immundefizit (thymus-deficient nude mouse, nu/nu-Kolonie) zu implantieren (1, 3).…”